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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Mouse IGF-2 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse IGF-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Igf2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IGF-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0381)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: A25-E91
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with IGF-1.
EK0381 is reactive to Igf2 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0381 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IGF-II/IGF2
Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.
|EK0381-CAP||Anti-Mouse Igf2 Pre-coated 96-well strip microplate||1|
|EK0381-ST||Mouse Igf2 Standard||2 vials, 10 ng/tube|
|EK0381-DA||Mouse Igf2 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse IGF-2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IGF-II/IGF2, Dilution ratio of 1:1, concentration in serum is 400-1000pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IGF-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of IGF-II/IGF2 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Igf2 (Source: Uniprot.Org, NCBI)
Insulin-like growth factor II
C11orf43; chromosome 11 open reading frame 43; FLJ22066; FLJ44734; GRDF; IGF2; IGF-2; IGFII; IGF-II; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor II; insulin-like growth factor type 2; MSA; PEG2; PP9974; somatomedin-A Igf2|AL033362, Igf, Igf-, Igf-2, Igf-II, M, M6, M6pr, Mpr, Peg, Peg2|insulin-like growth factor 2|insulin-like growth factor II|multiplication-stimulating polypeptide*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Igf2, check out the Igf2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Igf2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0381 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
IMP2 Increases Mouse Skeletal Muscle Mass and Voluntary Activity by Enhancing Autocrine Insulin-Like Growth Factor 2 Production and Optimizing Muscle Metabolism
Decline in IGF1 in the bone marrow microenvironment initiates hematopoietic stem cell aging
RNA m6A reader IMP2/IGF2BP2 promotes pancreatic β-cell proliferation and insulin secretion by enhancing PDX1 expression
Elevated insulin-like growth factor 2 expression may contribute to the hypermuscular phenotype of myostatin null mice
Regué L,Zhao L,Ji F,Wang H,Avruch J,Dai N. RNA m6A reader IMP2/IGF2BP2 promotes pancreatic β-cell proliferation and insulin secretion by enhancing PDX1 expression. Mol Metab. 2021 Mar 8:101209.doi:10.1016/j.molmet.2021.101209.Epub ahead of print.PMID:33705986.
EK0381 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Elevated insulin-like growth factor 2 expression may contribute to the hypermuscular phenotype of myostatin null mice.
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