Anti-MED1/Trap220 Antibody

Boster Bio Anti-MED1/Trap220 Antibody catalog # A00550. Tested in IHC, WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A00550
Size: 100μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: IHC, WB

Product Name

Anti-MED1/Trap220 Antibody

See all TRAP220/MED1 products

SKU/Catalog Number

A00550

Size

100μl

Form

Liquid

Description

Boster Bio Anti-MED1/Trap220 Antibody catalog # A00550. Tested in IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MED1/Trap220 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00550)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

Synthesized peptide derived from internal of human MED1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00550 is reactive to MED1 in Human, Mouse

Applications

A00550 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:3000
IHC, 1:50-1:100

Validation Images & Assay Conditions

Gene/Protein Information For MED1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q15648

Gene ID

5469

Gene Name

MED1

Full Name

Mediator of RNA polymerase II transcription subunit 1

Weight

168.478kDa

Superfamily

Mediator complex subunit 1 family

Alternative Names

Activator-recruited cofactor 205 kDa component; ARC205; CRSP1TRIP2; CRSP200p53 regulatory protein RB18A; DRIP230; DRIP230PPAR-binding protein; MBD4; MED1; mediator complex subunit 1DRIP205; mediator of RNA polymerase II transcription subunit 1; PBPPPARGBP; Peroxisome proliferator-activated receptor-binding protein; PPAR binding protein; PPARBP; PPARBPMGC71488; PPARG binding protein; PPARGBP; RB18ATR-interacting protein 2; Thyroid hormone receptor-associated protein complex 220 kDa component; thyroid hormone receptor-associated protein complex component TRAP220; thyroid receptor interacting pro MED1 CRSP1, CRSP200, DRIP205, DRIP230, PBP, PPARBP, PPARGBP, RB18A, TRAP220, TRIP2 mediator complex subunit 1 mediator of RNA polymerase II transcription subunit 1|ARC205|PPAR-binding protein|PPARG binding protein|TR-interacting protein 2|TRIP-2|activator-recruited cofactor 205 kDa component|p53 regulatory protein RB18A|peroxisome proliferator-activated receptor-binding protein|thyroid hormone receptor-associated protein complex 220 kDa component|thyroid hormone receptor-associated protein complex component TRAP220|thyroid receptor interacting protein 2|vitamin D receptor-interacting protein 230 kD|vitamin D receptor-interacting protein complex component DRIP205

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MED1, check out the MED1 Infographic

MED1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MED1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Anti-MED1/Trap220 Antibody

Question

Is this A00550 anti-MED1/Trap220 antibody reactive to the isotypes of MED1?

Verified Customer

Verified customer

Asked: 2020-04-13

Answer

The immunogen of A00550 anti-MED1/Trap220 antibody is Synthesized peptide derived from internal of human MED1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-13

Question

I see that the anti-MED1/Trap220 antibody A00550 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-01-13

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-01-13

Question

We are currently using anti-MED1/Trap220 antibody A00550 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

The anti-MED1/Trap220 antibody (A00550) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-26

Question

Will A00550 anti-MED1/Trap220 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-16

Answer

You can see on the product datasheet, A00550 anti-MED1/Trap220 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-16

Question

Does anti-MED1/Trap220 antibody A00550 work on canine WB with liver?

Verified Customer

Verified customer

Asked: 2017-12-20

Answer

Our lab technicians have not tested anti-MED1/Trap220 antibody A00550 on canine. You can run a BLAST between canine and the immunogen sequence of anti-MED1/Trap220 antibody A00550 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine liver in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-12-20

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
A00550
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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