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Product Info Summary
| SKU: | M00194-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MHC class 1 HLA-A Rabbit Monoclonal Antibody
SKU/Catalog Number
M00194-1
BM4084 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-MHC class 1 HLA-A Rabbit Monoclonal Antibody catalog # M00194-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MHC class 1 HLA-A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00194-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BBI-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human MHC class 1
Reactive Species
M00194-1 is reactive to HLA-A in Human
Observed Molecular Weight
41 kDa
Calculated molecular weight
40.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00194-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: human A549 whole cell,
human THP-1 whole cell,
human Jurkat whole cell,
human Raji whole cell,
human Daudi whole cell,
human A431 whole cell,
human HEL whole cell,
human RT4 whole cell
IHC: human pancreas cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of HLA-A using anti-HLA-A antibody (M00194-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human Raji whole cell lysates,
Lane 5: human Daudi whole cell lysates,
Lane 6: human A431 whole cell lysates,
Lane 7: human HEL whole cell lysates,
Lane 8: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA-A antigen affinity purified monoclonal antibody (M00194-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA-A at approximately 41 kDa. The expected band size for HLA-A is at 41 kDa.
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IHC analysis of HLA-A using anti-HLA-A antibody (M00194-1).
HLA-A was detected in a paraffin-embedded section of human pancreas cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-HLA-A Antibody (M00194-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-MHC class 1 HLA-A Rabbit Monoclonal Antibody (M00194-1)
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4 Customer Q&As for Anti-MHC class 1 HLA-A Rabbit Monoclonal Antibody
Question
My boss were content with the WB result of your anti-MHC class 1 Rabbit Monoclonal antibody. However we have seen positive staining in cervix carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is HLA-A supposed to be expressed?
J. Krishna
Verified customer
Asked: 2019-10-14
Answer
From what I have seen in literature, cervix carcinoma does express HLA-A. Generally HLA-A expresses in cell membrane. Regarding which tissues have HLA-A expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7836067, 8026990
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 23186163
Liver, Pubmed ID: 19159218, 24275569
Boster Scientific Support
Answered: 2019-10-14
Question
We have seen staining in human brain. Are there any suggestions? Is anti-MHC class 1 Rabbit Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2019-09-20
Answer
Based on literature brain does express HLA-A. Based on Uniprot.org, HLA-A is expressed in blood, brain, liver, cervix carcinoma, among other tissues. Regarding which tissues have HLA-A expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7836067, 8026990
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 23186163
Liver, Pubmed ID: 19159218, 24275569
Boster Scientific Support
Answered: 2019-09-20
Question
Our lab used your anti-MHC class 1 Rabbit Monoclonal antibody for IF on blood in the past. I am using human, and We want to use the antibody for WB next. We want examining blood as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
I looked at the website and datasheets of our anti-MHC class 1 Rabbit Monoclonal antibody and it seems that M00194-1 has been validated on human in both IF and WB. Thus M00194-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-25
Question
We are currently using anti-MHC class 1 Rabbit Monoclonal antibody M00194-1 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-09
Answer
The anti-MHC class 1 Rabbit Monoclonal antibody (M00194-1) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-09
