Mouse TNFRSF4/OX40 ELISA Kit PicoKine™

Mouse TNFRSF4/OX40 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfrsf4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0998
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Product Name

Mouse TNFRSF4/OX40 ELISA Kit PicoKine™

See all OX40/TNFRSF4 products

SKU/Catalog Number

EK0998

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse TNFRSF4/OX40 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfrsf4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse TNFRSF4/OX40 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0998)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: V20-P211

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0998 is reactive to Tnfrsf4 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Application Guarantee

EK0998 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0998-CAP Anti-Mouse Tnfrsf4 Pre-coated 96-well strip microplate 1
EK0998-ST Mouse Tnfrsf4 Standard 2 vials, 10 ng/tube
EK0998-DA Mouse Tnfrsf4 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0180.0520.1110.2170.3430.7051.2691.898

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect OX40/TNFRSF4, Dilution ratio of 1:1, concentration in serum is NO-20pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse TNFRSF4/OX40 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)10023389497255891
Standard deviation5.814.9139.335.7216.4243.65
CV (%)5.8%6.4%4.4%5.9%6.4%4.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of OX40/TNFRSF4 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1100928686915.746.3%
Sample 223321523125523314.236.1%
Sample 389498898798396339.884.1%
*number of samples for each test n=16.

Gene/Protein Information For Tnfrsf4 (Source: Uniprot.Org, NCBI)

Uniprot ID

P47741

Gene ID

22163

Gene Name

Tnfrsf4

Full Name

Tumor necrosis factor receptor superfamily member 4

Weight

30.154kDa

Alternative Names

ACT-135; ACT35 antigen; ACT35ATC35 antigen; CD134 antigen; CD134; Ly-70; OX40 cell surface antigen; OX40 homologue; OX40; OX40L receptor; OX40lymphoid activation antigene ACT35; TAX transcriptionally-activated glycoprotein 1 receptor; tax-transcriptionally activated glycoprotein 1 receptor; TNFRSF4; tumor necrosis factor receptor superfamily member 4; tumor necrosis factor receptor superfamily, member 4; Txgp1; TXGP1L; TXGP1LOX40 antigen Tnfrsf4|ACT3, ACT35, CD134, Ly-70, Ox4, Ox40, TXGP1L, Txgp, Txgp1|tumor necrosis factor receptor superfamily, member 4|tumor necrosis factor receptor superfamily member 4|OX40 antigen|OX40L receptor|tax-transcriptionally activated glycoprotein 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tnfrsf4, check out the Tnfrsf4 Infographic

Tnfrsf4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tnfrsf4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK0998

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Mouse TNFRSF4/OX40 ELISA Kit PicoKine™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Mouse TNFRSF4/OX40 ELISA Kit PicoKine™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

3 Customer Q&As for Mouse TNFRSF4/OX40 ELISA Kit PicoKine™

Question

Q: if the enzyme conjugated OX40 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the OX40 antigen?

L. Phillips

Verified customer

Asked: 2020-08-08

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-08-08

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring OX40 using the EK0998 Mouse OX40 Picokine® ELISA Kit?

M. Martinez

Verified customer

Asked: 2020-03-17

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2020-03-17

Question

Q: how to analyze ELISA data? I have obtained OX40 level in cell lysates.

R. Gandhi

Verified customer

Asked: 2019-06-06

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-06-06

$369

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
EK0998
$369.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.