Click image to see more details
Product Info Summary
| SKU: | M13360-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Mouse |
| Host: | Mouse |
| Application: | IP, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Ms Lime1 Purified Monoclonal Antibody
SKU/Catalog Number
M13360-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Ms Lime1 Purified Monoclonal Antibody (Catalog# M13360-1). Tested in IP, WB application(s). This antibody reacts with Mouse.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Ms Lime1 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M13360-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
mLIME-05
Isotype
Mouse IgG2a
Immunogen
Recombinant protein corresponding to amino acids 86-202 of mouse LIME. Mouse monoclonal antibody mLIME-05 reacts with the cytoplasmic domain of LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed mainly by T cells.
Cross-reactivity
As in case with other anti-CD170 antibodies, this antibody cross-reacts with Siglec-14, whose first two Ig domains are almost identical to those of CD170.
Reactive Species
M13360-1 is reactive to Lime1 in Mouse
Observed Molecular Weight
42 kDa
Calculated molecular weight
29.6 kDa
Background of Lime1
LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M13360-1 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunoprecipitation: Positive control: murine splenic T cells.
Western blotting: Reducing conditions, recommended dilution: 1-5 μg/ml.
Validation Images & Assay Conditions
Click image to see more details
Western blotting analysis of murine thymocyte lysate (1% SDS); non-reduced sample, immunostained by mAb mLIME-05 and goat anti-mouse IgG (H+L)-HRP conjugate.
Specific Publications For Anti-Ms Lime1 Purified Monoclonal Antibody (M13360-1)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Ms Lime1 Purified Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Ms Lime1 Purified Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
