Anti-FK506-binding protein 15 FKBP15 Antibody

Product Name

Anti-FK506-binding protein 15 FKBP15 Antibody

View all FKBP15 Antibodies

SKU/Catalog Number

A10447

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-FK506-binding protein 15 FKBP15 Antibody (Catalog # A10447). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

FKBP15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-FK506-binding protein 15 FKBP15 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10447)

Host

Rabbit

Contents

FKBP15 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

FKBP15 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human FKBP15. The immunogen is located within amino acids 30 - 80 of FKBP15.

Reactive Species

A10447 is reactive to FKBP15 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

133.6 kDa

Background of FKBP15

FKBP15, also known as FKBP133, is a member of the FK506-binding protein family, a group of proteins initially identified as immunophilins, targets for the immunosupressant drugs FK506 and Rapamycin. FKBP15 is expressed in the developing nervous system and contains a domain similar to Wiskott-Aldrich syndrome protein homology region 1 (WH1) in addition to the FK506-binding protein motif. FKBP15 is distributed along the axonal shafts and partially co-localizes with F-actin in the growth cones of dorsal root ganglion neurons; overexpression of FKBP15 resulted in the number of filopodia in transfected neurons, suggesting that FKBP15 modulates growth cone behavior. FKBP15 has also been shown to associate with both microtubules and the actin filament systems and disruption of its expression by RNAi resulted in delayed transport of early endosomes in HeLa cells indicating that FKBP15 is also involved in the transport of early endosomes. At least three isoforms of FKBP15 are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A10447 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

FKBP15 antibody can be used for detection of FKBP15 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information FKBP15

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