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Product Info Summary
| SKU: | DZ11633 |
|---|---|
| Size: | 200 μl/vial |
| Reactive Species: | Zebrafish |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Zebrafish wfikkn1 Antibody
SKU/Catalog Number
DZ11633
Size
200 μl/vial
Form
Liquid
Description
Boster Bio Anti-wfikkn1 Antibody catalog # DZ11633. This antibody reacts with Zebrafish.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Zebrafish wfikkn1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # DZ11633)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Reactive Species
DZ11633 is reactive to wfikkn1 in Zebrafish
Observed Molecular Weight
62 kDa
Application & Images
Applications
DZ11633 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5 μg/ml
Positive Control
WB: zebrafish, zebrafish head tissue, zebrafish body tissue.
Validation Images & Assay Conditions
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Western blot analysis of Wfikkn1 using anti-Wfikkn1 antibody (DZ11633).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Zebrafish,
Lane 2: Zebrafish head tissue lysates,
Lane 3: Zebrafish body tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Wfikkn1 antigen affinity purified polyclonal antibody (DZ11633) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Wfikkn1 at approximately 62 kDa. The expected band size for Wfikkn1 is at 62 kDa.
Specific Publications For Anti-Zebrafish wfikkn1 Antibody (DZ11633)
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