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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-LBP Antibody Picoband™
Boster Bio Anti-LBP Antibody Picoband™ catalog # A00809-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-LBP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00809-1)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E.coli-derived human LBP recombinant protein (Position: L177-E446). Human LBP shares 68.5% and 69.6% amino acid (aa) sequence identity with mouse and rat LBP, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A00809-1 is reactive to LBP in Human, Mouse, Rat
A00809-1 is guaranteed for WB Boster Guarantee
Background of LBP
Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. Gram-negative bacteria contain a glycolipid, lipopolysaccharide (LPS), on their outer cell wall. Together with bactericidal permeability-increasing protein (BPI), the encoded protein binds LPS and interacts with the CD14 receptor, probably playing a role in regulating LPS-dependent monocyte responses. Studies in mice suggest that the encoded protein is necessary for the rapid acute-phase response to LPS but not for the clearance of LPS from circulation. This protein is part of a family of structurally and functionally related proteins, including BPI, plasma cholesteryl ester transfer protein (CETP), and phospholipid transfer protein (PLTP).
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of LBP using anti-LBP antibody (A00809-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat thymus tissue lysates,
Lane 2: mouse thymus tissue lysates,
Lane 3: HELA whole cell lysates,
Lane 4: K562 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LBP antigen affinity purified polyclonal antibody (Catalog # A00809-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for LBP at approximately 65KD. The expected band size for LBP is at 53KD.
Gene/Protein Information For LBP (Source: Uniprot.Org, NCBI)
LBP; lipopolysaccharide binding protein; lipopolysaccharide-binding protein; LPS-binding protein; MGC22233 LBP BPIFD2 lipopolysaccharide binding protein lipopolysaccharide-binding protein|BPI fold containing family D, member 2|LPS-binding protein*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on LBP, check out the LBP Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LBP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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