Human MMP-3/Stromelysin-1 ELISA Kit PicoKine™

Human MMP-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP3 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. Cited in 13 publication(s).

Product Info Summary

SKU: EK0461
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin).

Product Name

Human MMP-3/Stromelysin-1 ELISA Kit PicoKine™

See all MMP-3 products

SKU/Catalog Number

EK0461

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human MMP-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP3 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human MMP-3/Stromelysin-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0461)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y18-C477

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is cross-reactivity with MMP-10 approximately 2%, and no detectable cross-reactivity with other MMPs.

Reactive Species

EK0461 is reactive to MMP3 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

EK0461 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0461-CAP Anti-Human MMP3 Pre-coated 96-well strip microplate 1
EK0461-ST Human MMP3 Standard 2 vials, 10 ng/tube
EK0461-DA Human MMP3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0410.1680.2580.4690.9431.4831.9822.319

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human MMP-3/Stromelysin-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2841539465631016564415
Standard deviation14.48100.03297.9819.22112.6304.63
CV (%)6.9%6.5%6.4%6.2%6.8%6.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of MMP-3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 128430030025528418.376.4%
Sample 21539157216751602159750.243.1%
Sample 346563964455245074419268.616%
*number of samples for each test n=16.

Gene/Protein Information For MMP3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P08254

Gene ID

4314

Gene Name

MMP3

Full Name

Stromelysin-1

Weight

53.977kDa

Superfamily

peptidase M10A family

Alternative Names

CHDS6; EC 3.4.24; EC 3.4.24.17; matrix metallopeptidase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3 (stromelysin 1, progelatinase); Matrix metalloproteinase-3; MGC126102; MGC126103; MMP3; MMP-3; proteoglycanase; SL-1; STMY; STMY1MGC126104; STR1; Stromelysin 1; stromelysin-1; transin-1 MMP3 CHDS6, MMP-3, SL-1, STMY, STMY1, STR1 matrix metallopeptidase 3 stromelysin-1|matrix metalloproteinase 3 (stromelysin 1, progelatinase)|proteoglycanase|transin-1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MMP3, check out the MMP3 Infographic

MMP3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MMP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0461 has been cited in 13 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Singh A,Banerjee T,Shukla SK.Factors Associated with High Rates of Recurrence of Amebic Liver Abscess (ALA) in North India.Am J Trop Med Hyg.2021 Jan 11.doi:10.4269/ajtmh.20-0074.Epub ahead of print.PMID:33432901.
Species: Human
EK0461 usage in article: APP:ELISA, SAMPLE:LIVER ASPIRATE, DILUTION:NA

Mishra D,Dhir V,Naidu GSRSNK,Khullar A,Kumar V,Sharma S,Sharma A,Jain S. Efficacy of a step-down regimen of oral prednisolone in axial spondyloarthritis: result of a double-blind randomized controlled trial (COBRA-AS Study). Rheumatol ogy(Oxford).2020 Nov
Species: Human
EK0461 usage in article: APP:ELISA, SAMPLE:HUMAN SERUM, DILUTION:NA

Carvacrol suppresses inflammatory responses in rheumatoid arthritis fibroblast-like synoviocytes

Comparison of Intra-articular Injection of Hyaluronic Acid and N-Acetyl Cysteine in the Treatment of Knee Osteoarthritis: A Pilot Study

Salivary MMP-1, MMP-2, MMP-3 and MMP-13 Levels in Patients with Oral Lichen Planus and Squamous Cell Carcinoma

P2X7 Mediates ATP-Driven Invasiveness in Prostate Cancer Cells

Vitiligo: A Possible Model of Degenerative Diseases

Detection and comparison of matrix metalloproteinase in primary and recurrent pterygium fibroblasts

Lin, H., Qiu, S., Xie, L., Liu, C., & Sun, S. (2017). Nimbolide suppresses non-small cell lung cancer cell invasion and migration via manipulation of DUSP4 expression and ERK1/2 signaling. Biomedicine & Pharmacotherapy, 92, 340-346. Advance online...

Zhao J, Fan X, Zhang Q, Sun F, Li X, Xiong C, Zhang C, Fan H. J Biomed Mater Res A. 2014 Feb;102(2):373-80. Doi: 10.1002/Jbm.A.34711. Epub 2013 May 14. Chitosan-Plasmid Dna Nanoparticles Encoding Small Hairpin Rna Targeting Mmp-3 And -13 To Inhibi...

Zhu F, Liu P, Li J, Zhang Y. Oncol Rep. 2014 May;31(5):2049-54. Doi: 10.3892/Or.2014.3060. Epub 2014 Mar 5. Eotaxin-1 Promotes Prostate Cancer Cell Invasion Via Activation Of The Ccr3-Erk Pathway And Upregulation Of Mmp-3 Expression.

??gren Ms, Schnabel R, Christensen Lh, Mirastschijski U. Eur J Cell Biol. 2015 Jan;94(1):12-21. Doi: 10.1016/J.Ejcb.2014.10.001. Epub 2014 Oct 23. Tumor Necrosis Factor-??-Accelerated Degradation Of Type I Collagen In Human Skin Is Associated With...

Capsoni F, Ongari Am, Lonati C, Accetta R, Gatti S, Catania A. Bmc Musculoskelet Disord. 2015 Jun 21;16:154. Doi: 10.1186/S12891-015-0615-1. ??-Melanocyte-Stimulating-Hormone (??-Msh) Modulates Human Chondrocyte Activation Induced By Proinflammato...

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4 Customer Q&As for Human MMP-3/Stromelysin-1 ELISA Kit PicoKine™

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

B. Parker

Verified customer

Asked: 2019-05-12

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-05-12

Question

Q: What is the optimal O.D. value for MMP-3 ELISA kit? I used your MMP-3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain MMP-3 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-04-12

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this MMP-3 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of MMP-3 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-04-12

Question

Q: how to thaw whole blood sample for MMP-3 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-01-16

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test MMP-3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-01-16

Question

Do these ELISA kits (Human MMP-2 & Human MMP-3) use the same sample diluent? What kind of chemicals are present in this diluent?

Verified Customer

Verified customer

Asked: 2016-07-07

Answer

Yes. The diluent contains NaCl, Tris, BSA and preservative.

Boster Scientific Support

Answered: 2016-07-07

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