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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin).|
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Human MMP-3/Stromelysin-1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human MMP-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP3 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MMP-3/Stromelysin-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0461)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: Y18-C477
156 pg/ml - 10,000 pg/ml
There is cross-reactivity with MMP-10 approximately 2%, and no detectable cross-reactivity with other MMPs.
EK0461 is reactive to MMP3 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
EK0461 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MMP-3
Matrix metalloproteinase-3 (MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase (MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54% identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures. MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition. The standard product used in this kit is recombinant human MMP-3, consisting of 460 amino acids with the molecular mass of 52KDa. The detected MMP-3 includes zymogen and active enzyme.
|EK0461-CAP||Anti-Human MMP3 Pre-coated 96-well strip microplate||1|
|EK0461-ST||Human MMP3 Standard||2 vials, 10 ng/tube|
|EK0461-DA||Human MMP3 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human MMP-3 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MMP-3/Stromelysin-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of MMP-3 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For MMP3 (Source: Uniprot.Org, NCBI)
peptidase M10A family
CHDS6; EC 3.4.24; EC 22.214.171.124; matrix metallopeptidase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3 (stromelysin 1, progelatinase); Matrix metalloproteinase-3; MGC126102; MGC126103; MMP3; MMP-3; proteoglycanase; SL-1; STMY; STMY1MGC126104; STR1; Stromelysin 1; stromelysin-1; transin-1 MMP3 CHDS6, MMP-3, SL-1, STMY, STMY1, STR1 matrix metallopeptidase 3 stromelysin-1|matrix metalloproteinase 3 (stromelysin 1, progelatinase)|proteoglycanase|transin-1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on MMP3, check out the MMP3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MMP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0461 has been cited in 14 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Neutrophil membrane-coated nanoparticles inhibit synovial inflammation and alleviate joint damage in inflammatory arthritis
Singh A,Banerjee T,Shukla SK.Factors Associated with High Rates of Recurrence of Amebic Liver Abscess (ALA) in North India.Am J Trop Med Hyg.2021 Jan 11.doi:10.4269/ajtmh.20-0074.Epub ahead of print.PMID:33432901.
EK0461 usage in article: APP:ELISA, SAMPLE:LIVER ASPIRATE, DILUTION:NA
Mishra D,Dhir V,Naidu GSRSNK,Khullar A,Kumar V,Sharma S,Sharma A,Jain S. Efficacy of a step-down regimen of oral prednisolone in axial spondyloarthritis: result of a double-blind randomized controlled trial (COBRA-AS Study). Rheumatol ogy(Oxford).2020 Nov
EK0461 usage in article: APP:ELISA, SAMPLE:HUMAN SERUM, DILUTION:NA
Carvacrol suppresses inflammatory responses in rheumatoid arthritis fibroblast-like synoviocytes
Comparison of Intra-articular Injection of Hyaluronic Acid and N-Acetyl Cysteine in the Treatment of Knee Osteoarthritis: A Pilot Study
Salivary MMP-1, MMP-2, MMP-3 and MMP-13 Levels in Patients with Oral Lichen Planus and Squamous Cell Carcinoma
P2X7 Mediates ATP-Driven Invasiveness in Prostate Cancer Cells
Vitiligo: A Possible Model of Degenerative Diseases
Detection and comparison of matrix metalloproteinase in primary and recurrent pterygium fibroblasts
Lin, H., Qiu, S., Xie, L., Liu, C., & Sun, S. (2017). Nimbolide suppresses non-small cell lung cancer cell invasion and migration via manipulation of DUSP4 expression and ERK1/2 signaling. Biomedicine & Pharmacotherapy, 92, 340-346. Advance online...
Zhao J, Fan X, Zhang Q, Sun F, Li X, Xiong C, Zhang C, Fan H. J Biomed Mater Res A. 2014 Feb;102(2):373-80. Doi: 10.1002/Jbm.A.34711. Epub 2013 May 14. Chitosan-Plasmid Dna Nanoparticles Encoding Small Hairpin Rna Targeting Mmp-3 And -13 To Inhibi...
Zhu F, Liu P, Li J, Zhang Y. Oncol Rep. 2014 May;31(5):2049-54. Doi: 10.3892/Or.2014.3060. Epub 2014 Mar 5. Eotaxin-1 Promotes Prostate Cancer Cell Invasion Via Activation Of The Ccr3-Erk Pathway And Upregulation Of Mmp-3 Expression.
??gren Ms, Schnabel R, Christensen Lh, Mirastschijski U. Eur J Cell Biol. 2015 Jan;94(1):12-21. Doi: 10.1016/J.Ejcb.2014.10.001. Epub 2014 Oct 23. Tumor Necrosis Factor-??-Accelerated Degradation Of Type I Collagen In Human Skin Is Associated With...
Capsoni F, Ongari Am, Lonati C, Accetta R, Gatti S, Catania A. Bmc Musculoskelet Disord. 2015 Jun 21;16:154. Doi: 10.1186/S12891-015-0615-1. ??-Melanocyte-Stimulating-Hormone (??-Msh) Modulates Human Chondrocyte Activation Induced By Proinflammato...
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