Human MMP-3/Stromelysin-1 ELISA Kit PicoKine®

MMP-3 ELISA kit for Human

Human MMP-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP3 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. Cited in 10 publication(s).

Product Info Summary

SKU: EK0461
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin).

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Product Name

Human MMP-3/Stromelysin-1 ELISA Kit PicoKine®

View all MMP-3 ELISA kits

SKU/Catalog Number

EK0461

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human MMP-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human MMP3 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human MMP-3/Stromelysin-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0461)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y18-C477

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross-reactivity

There is cross-reactivity with MMP-10 approximately 2%, and no detectable cross-reactivity with other MMPs.

Reactive Species

EK0461 is reactive to MMP3 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

EK0461 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of MMP-3

Matrix metalloproteinase-3 (MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase (MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54% identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures. MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition. The standard product used in this kit is recombinant human MMP-3, consisting of 460 amino acids with the molecular mass of 52KDa. The detected MMP-3 includes zymogen and active enzyme.

Kit Components

Catalog Number Description Quantity
EK0461-CAP Anti-Human MMP3 Pre-coated 96-well strip microplate 1
EK0461-ST Human MMP3 Standard 2 vials, 10 ng/tube
EK0461-DA Human MMP3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0410.0830.1680.2580.4690.9431.4831.982

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human MMP-3/Stromelysin-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2841539465631016564415
Standard deviation14.48100.03297.9819.22112.6304.63
CV (%)6.9%6.5%6.4%6.2%6.8%6.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of MMP-3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 128430030025528418.376.4%
Sample 21539157216751602159750.243.1%
Sample 346563964455245074419268.616%
*number of samples for each test n=16.

Gene/Protein Information For MMP3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P08254

Gene ID

4314

Gene Name

MMP3

Full Name

Stromelysin-1

Weight

53.977kDa

Superfamily

peptidase M10A family

Alternative Names

CHDS6; EC 3.4.24; EC 3.4.24.17; matrix metallopeptidase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3 (stromelysin 1, progelatinase); Matrix metalloproteinase-3; MGC126102; MGC126103; MMP3; MMP-3; proteoglycanase; SL-1; STMY; STMY1MGC126104; STR1; Stromelysin 1; stromelysin-1; transin-1 MMP3 CHDS6, MMP-3, SL-1, STMY, STMY1, STR1 matrix metallopeptidase 3 stromelysin-1|matrix metalloproteinase 3 (stromelysin 1, progelatinase)|proteoglycanase|transin-1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MMP3, check out the MMP3 Infographic

MMP3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MMP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0461 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

HOXB5 promotes retinoblastoma cell migration and invasion via ERK1/2 pathway-mediated MMPs production

Detection and comparison of matrix metalloproteinase in primary and recurrent pterygium fibroblasts

Effects of exercise therapy on knee joint function and synovial fluid cytokine levels in patients with knee osteoarthritis

A Combination of Celecoxib and Glucosamine Sulfate Has Anti-Inflammatory and Chondroprotective Effects: Results from an In Vitro Study on Human Osteoarthritic Chondrocytes

P2X7 Mediates ATP-Driven Invasiveness in Prostate Cancer Cells

Vitiligo: A Possible Model of Degenerative Diseases

Clinical value of matrix metalloproteinase-2 and -9 in ultrasound-guided radiofrequency ablation treatment for papillary thyroid carcinoma:

Efficacy of a step-down regimen of oral prednisolone in axial spondyloarthritis: result of a double-blind randomized controlled trial (COBRA-AS Study)

Heme oxygenase-1 attenuates IL-1β induced alteration of anabolic and catabolic activities in intervertebral disc degeneration

Anti-inflammatory and anti-arthritic effects of the ethanolic extract of Aralia continentalis Kitag. in IL-1β-stimulated human fibroblast-like synoviocytes and rodent models of polyarthritis and nociception

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4 Customer Q&As for Human MMP-3/Stromelysin-1 ELISA Kit PicoKine®

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

B. Parker

Verified customer

Asked: 2019-05-12

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-05-12

Question

Q: What is the optimal O.D. value for MMP-3 ELISA kit? I used your MMP-3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain MMP-3 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-04-12

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this MMP-3 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of MMP-3 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-04-12

Question

Q: how to thaw whole blood sample for MMP-3 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-01-16

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test MMP-3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-01-16

Question

Do these ELISA kits (Human MMP-2 & Human MMP-3) use the same sample diluent? What kind of chemicals are present in this diluent?

Verified Customer

Verified customer

Asked: 2016-07-07

Answer

Yes. The diluent contains NaCl, Tris, BSA and preservative.

Boster Scientific Support

Answered: 2016-07-07

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