Anti-NADE BEX3 Antibody

Product Name

Anti-NADE BEX3 Antibody

View all NGFRAP1 Antibodies

SKU/Catalog Number

A09675

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-NADE BEX3 Antibody (Catalog # A09675). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse.

Storage & Handling

NADE antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-NADE BEX3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09675)

Host

Rabbit

Contents

NADE Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

NADE antibody was raised against a peptide corresponding to 14 amino acids near the middle of human NADE. The immunogen is located within the last 50 amino acids of NADE.

Reactive Species

A09675 is reactive to NGFRAP1 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

13.0 kDa

Background of NGFRAP1

The p75 neurotrophin receptor (p75NTR) is a member of the tumor necrosis receptor superfamily and can mediate cell death and cell survival in response to nerve growth factor (NGF). The p75NTR-associated cell death executor (NADE) mediates apoptosis by interacting with the cell death domain of p75NTR following the binding of NGF by p75NTR. Recent studies have shown that NADE also interacts with second mitochondria-derived activator of caspase (Smac). Co-expression of NADE and Smac promotes TRAIL-induced apoptosis and inhibits XIAP-mediated Smac ubiquitization. It has been suggested that it is this interaction between NADE and Smac that allows apoptosis to proceed. Finally, although initially discovered as an mRNA expressed in ovarian granulosa cells, NADE has been suggested to play a role in the neuronal death seen in epileptic brain damage.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A09675 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

NADE antibody can be used for detection of NADE by Western blot at 1 μg/mL. Despite its predicted molecular weight, NADE migrates at ~23 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information NGFRAP1

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