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Product Info Summary
| SKU: | M05685-1 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Napsin A (Lung Adenocarcinoma Marker) NAPSA Monoclonal Antibody
SKU/Catalog Number
M05685-1
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Napsin A (Lung Adenocarcinoma Marker) NAPSA Monoclonal Antibody (Catalog # M05685-1). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Napsin A (Lung Adenocarcinoma Marker) NAPSA Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05685-1)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: NAPSA/1238 + NAPSA/1239
Isotype
IgG, kappa
Immunogen
Recombinant human Napsin-A protein fragment (aa189-299) (exact sequence is proprietary)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M05685-1 is reactive to NAPSA in Human
Applications
M05685-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
45.387kDa
Background of Napsin A
Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml for 60 minutes at RT)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Lung Adenocarcinoma stained with Anti-Napsin-A Monoclonal Antibody (NAPSA/1238 + NAPSA/1239).
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Western Blot of K562, HEK293 andA549 cell lysates using Anti-Napsin-A Monoclonal Antibody (NAPSA/1238 + NAPSA/1239)
Protein Target Info & Infographic
Gene/Protein Information For NAPSA (Source: Uniprot.org, NCBI)
Gene Name
NAPSA
Full Name
Napsin-A
Weight
45.387kDa
Superfamily
peptidase A1 family
Alternative Names
Asp 4; ASP4; Aspartyl protease 4; EC 3.4.23; EC 3.4.23.-; EC 3.4.23.15; EC 3.4.23.3; EC 3.4.23.5; KAP; Kdap; NAP1napsin-A; NAPA; NAPApronapsin A; NAPSA; napsin A aspartic peptidase; Napsin A; Napsin-1; SNAPA; TA01/TA02 NAPSA KAP, Kdap, NAP1, NAPA, SNAPA napsin A aspartic peptidase napsin-A|ASP4|TA01/TA02|asp 4|aspartyl protease 4|kidney-derived aspartic protease-like protein|napsin-1|pronapsin A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NAPSA, check out the NAPSA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NAPSA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Napsin A (Lung Adenocarcinoma Marker) NAPSA Monoclonal Antibody (M05685-1)
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