Anti-NCAM1 Antibody Picoband™

NCAM-1/CD56 antibody

Boster Bio Anti-NCAM1 Antibody Picoband™ catalog # A00184. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A00184
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-NCAM1 Antibody Picoband™

View all NCAM-1/CD56 Antibodies

SKU/Catalog Number

A00184

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-NCAM1 Antibody Picoband™ catalog # A00184. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NCAM1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00184)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human NCAM1 recombinant protein (Position: K152-Q690).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00184 is reactive to NCAM1 in Human, Mouse, Rat

Applications

A00184 is guaranteed for ELISA, WB Boster Guarantee

Background of NCAM-1/CD56

NCAM is a membrane-bound glycoprotein that plays a role in cell-cell and cell-matrix adhesion through both its homophilic and heterophilic binding activity. The neural cell adhesion molecule appears on early embryonic cells and is important in the formation of cell collectives and their boundaries at sites of morphogenesis. Later in development it is found on various differentiated tissues and is a major CAM mediating adhesion among neurons and between neurons and muscle. NCAM gene is mapped to 11q23. The neural cell adhesion molecule (NCAM) can influence a number of diverse intercellular events, including junctional communication, the association of axons with pathways and targets, and signals that alter levels of neurotransmitter enzymes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25μg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For NCAM1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P13591

Gene ID

4684

Gene Name

NCAM1

Full Name

Neural cell adhesion molecule 1

Weight

94.574kDa

Alternative Names

CD56 / NCAM-1; CD56 antigen; CD56; MSK39; NCAM1; N-CAM-1; NCAM-1; NCAMantigen recognized by monoclonal 5.1H11; neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM NCAM1 CD56, MSK39, NCAM neural cell adhesion molecule 1 neural cell adhesion molecule 1|antigen recognized by monoclonal antibody 5.1H11|neural cell adhesion molecule, NCAM

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on NCAM1, check out the NCAM1 Infographic

NCAM1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for NCAM1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00184 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Assessment of the innate and adaptive immune system in proliferative vitreoretinopathy

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5 Customer Q&As for Anti-NCAM1 Antibody Picoband™

Question

We are currently using anti-NCAM1 antibody A00184 for rat tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-02

Answer

The anti-NCAM1 antibody (A00184) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-02

Question

We want using your anti-NCAM1 antibody for ncam1 interactions studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

We appreciate your inquiry. This A00184 anti-NCAM1 antibody is tested on rat brain tissue, mouse brain, u937 cells. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-05-21

Question

My boss were satisfied with the WB result of your anti-NCAM1 antibody. However we have observed positive staining in neocortex isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is NCAM1 supposed to be expressed?

D. Bhatt

Verified customer

Asked: 2015-01-16

Answer

According to literature, neocortex does express NCAM1. Generally NCAM1 expresses in isoform 1: cell membrane. Regarding which tissues have NCAM1 expression, here are a few articles citing expression in various tissues:
Brain, Kidney, and Ovary, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Plasma, Pubmed ID: 16335952
Skeletal muscle, Pubmed ID: 2887295, 3253057

Boster Scientific Support

Answered: 2015-01-16

Question

We have been able to see staining in mouse liver. Are there any suggestions? Is anti-NCAM1 antibody supposed to stain liver positively?

G. Carter

Verified customer

Asked: 2014-10-20

Answer

From literature liver does express NCAM1. From Uniprot.org, NCAM1 is expressed in neocortex, skeletal muscle, testis, brain, brain, kidney ovary, plasma, liver, among other tissues. Regarding which tissues have NCAM1 expression, here are a few articles citing expression in various tissues:
Brain, Kidney, and Ovary, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Plasma, Pubmed ID: 16335952
Skeletal muscle, Pubmed ID: 2887295, 3253057

Boster Scientific Support

Answered: 2014-10-20

Question

We have tried in the past anti-NCAM1 antibody for WB on testis last year. I am using mouse, and I plan to use the antibody for ELISA next. I am interested in examining testis as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?

G. Singh

Verified customer

Asked: 2014-09-22

Answer

I have checked the website and datasheets of our anti-NCAM1 antibody and it appears that A00184 has been validated on mouse in both WB and ELISA. Thus A00184 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2014-09-22

Conjugation

how to order through PO

Size

Total: $315

Size:100μg

SKU:A00184

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A00184
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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