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- Table of Contents
SYP antibody (A05049) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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IF analysis of SYP using anti-SYP antibody (PB9426).
SYP was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SYP2 Antibody (PB9426) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
SYP was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-SYP2 Antibody (PB9426) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (BA1003) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Synaptophysin/SYP Picoband® Antibody
SYP antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
SYP antibody was positively detected by WB in:
rat brain tissue, rat hippocampus tissue, mouse brain tissue, mouse hippocampus tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SYP antibody was positively detected by IHC in:
human appendicitis tissue, human glioma tissue, mouse pancreas tissue, rat pancreas tissue, mouse brain tissue, rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
SYP antibody was positively detected by ICC/IF in:
SH-SY5Y cell
SYP antibody was positively detected by IF in:
mouse pancreas tissue, rat pancreas tissue, mouse brain tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25μg/ml
Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section
2-5 μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5μg/ml
Human
Immunofluorescence
5μg/ml
Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Rat
Tested
Tested
Tested
Mouse
Tested
Tested
Tested
Human
Tested
Recommend
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Synaptophysin/SYP Picoband® Antibody
View all SYP Antibodies
View all SYP Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Synaptophysin/SYP, which shares 91.3% amino acid (aa) sequence identity with both mouse and rat Synaptophysin/SYP.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
38 kDa
Calculated Molecular Weight
33.8 kDa
Background of SYP
Synaptophysin, also known as the major synaptic vesicle protein p38, is a protein that in humans is encoded by the SYP gene. It is mapped to Xp11.23. This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with an X-linked form of cognitive disability.
Cite This Product
Anti-Synaptophysin/SYP Picoband® Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05049)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Mouse
84%
Moderate Homology
Rat
84%
Moderate Homology
Cow
84%
Moderate Homology
Sheep
84%
Moderate Homology
Zebrafish
84%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SYP antibody
Immunohistochemistry (IHC) protocol for SYP antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for SYP antibody
Immunofluorescence (IF) protocol for SYP antibody
Publications
Application:
Species:
Exposure to intermittent hypoxia impairs learning and memory ability in rats
Published: 2018-03-31
Journal: Sleep and Biological Rhythms
Application: IHC
Impact Factor: 0.66
Species: Rat
Effects of spontaneous recurrent seizures on cognitive function via modulation of SNAREs expression
Published: 2017-10-23
Journal: INTERNATIONAL JOURNAL OF NEUROSCIENCE
Application: WB
Impact Factor: 1.75
Species: Rat
PMID: 29057696
The induction of cytochrome P450 2E1 by ethanol leads to the loss of synaptic proteins via PPARα down-regulation
Published: 2017-04-03
Journal: TOXICOLOGY
Application: ICC
Impact Factor: 3.58
Species: Human
PMID: 28385499
Downregulation of caveolin-1 contributes to the synaptic plasticity deficit in the hippocampus of aged rats
Published: 2013-10-10
Journal: Neural Regeneration Research
Application: WB
Impact Factor: 0.14
Species: Rat
PMID: 25206583
Pentamethylquercetin Protects Against Diabetes-Related Cognitive Deficits in Diabetic Goto-Kakizaki Rats
Published: 2013-03-04
Journal: JOURNAL OF ALZHEIMERS DISEASE
Application: WB,IHC
Impact Factor: 4.17
Species: Rat
PMID: 23271319
Generation of Retinal Ganglion–like Cells from Reprogrammed Mouse Fibroblasts
Published: 2010-11-01
Journal: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
Application: IF
Impact Factor: 3.43
Species: Mouse
PMID: 20484577
Catalpol increases hippocampal neuroplasticity and up-regulates PKC and BDNF in the aged rats
Published: 2006-10-31
Journal: BRAIN RESEARCH
Application: WB,IHC
Impact Factor: 2.30
Species: Rat
PMID: 17078935
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Product Promise
We promise Anti-Synaptophysin/SYP Picoband® Antibody performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A05049
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026