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- Table of Contents
SYP antibody (PB9409) performs well in WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 8 Publications
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Western blot analysis of Synaptophysin/SYP using anti-Synaptophysin/SYP antibody (PB9409).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat hippocampus tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse hippocampus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synaptophysin/SYP antigen affinity purified polyclonal antibody (PB9409) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Synaptophysin/SYP at approximately 38 kDa. The expected band size for Synaptophysin/SYP is at 34 kDa.
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat hippocampus tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse hippocampus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synaptophysin/SYP antigen affinity purified polyclonal antibody (PB9409) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Synaptophysin/SYP at approximately 38 kDa. The expected band size for Synaptophysin/SYP is at 34 kDa.
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Anti-Synaptophysin/SYP Antibody Picoband®
SYP antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | WB |
Tested Applications
SYP antibody was positively detected by WB in:
rat brain tissue, rat hippocampus tissue, mouse brain tissue, mouse hippocampus tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Mouse, Rat, Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
Rat
Tested
Mouse
Tested
Human
Predicted
Bovine
Predicted
Sheep
Predicted
Dog
Predicted
Monkey
Predicted
Product Information
Product Name
Anti-Synaptophysin/SYP Antibody Picoband®
View all SYP Antibodies
View all SYP Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Synaptophysin, identical to the related mouse and rat sequences.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
38 kDa
Calculated Molecular Weight
33.8 kDa
Background of SYP
SYP (Synaptophysin) is located on the short arm of X chromosome (Xp11.23-p11.22). The protein is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38kDa. It is present inneuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. It acts as a marker for neuroendocrine tumors, and its ubiquity at the synapse has led to the use of synaptophysin immunostaining for quantification of synapses. Also, the protein binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR).
Cite This Product
Anti-Synaptophysin/SYP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9409)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Rat
77%
Moderate Homology
Zebrafish
58%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SYP antibody
Publications
Application:
Species:
Morroniside Modulates Microglia Polarization via the CX3CL1/CX3CR1/PU.1 Axis in ApoE4 Transgenic Mice
Published: 2026-01-08
Journal: PHYTOTHERAPY RESEARCH
Application: WB
Impact Factor: 6.30
Species: Mouse
PMID: 41502346
Ketogenic diet attenuates cognitive dysfunctions induced by hypoglycemia via inhibiting endoplasmic reticulum stress-dependent pathways
Published: 2023-12-21
Journal: Food & Function
Application: WB
Impact Factor: 6.10
Species: Mouse
PMID: 38197246
Myricetin improves pathological changes in 3×Tg-AD mice by regulating the mitochondria-NLRP3 inflammasome-microglia channel by targeting P38 MAPK signaling pathway
Published: 2023-04-06
Journal: PHYTOMEDICINE
Application: WB
Impact Factor: 7.90
Species: Mouse
PMID: 37086707
Loganin alleviated cognitive impairment in 3×Tg-AD mice through promoting mitophagy mediated by optineurin
Published: 2023-04-04
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 5.40
Species: Mouse
PMID: 37019163
Baicalein ameliorates Alzheimer's disease via orchestration of CX3CR1/NF-κB pathway in a triple transgenic mouse model
Published: 2023-03-14
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 5.60
Species: Mouse
PMID: 37098656
3, 14, 19-Triacetyl Andrographolide alleviates the cognitive dysfunction of 3 × Tg-AD mice by inducing initiation and promoting degradation process of autophagy
Published: 2022-09-17
Journal: PHYTOTHERAPY RESEARCH
Application: WB
Impact Factor: 6.39
Species: Mouse
PMID: 36114804
Cornuside Is a Potential Agent against Alzheimer’s Disease via Orchestration of Reactive Astrocytes
Published: 2022-08-03
Journal: Nutrients
Application: WB
Impact Factor: 6.71
Species: Mouse
PMID: 35956355
ATP11B deficiency leads to impairment of hippocampal synaptic plasticity
Published: 2019-06-01
Journal: Journal of Molecular Cell Biology
Application: WB
Impact Factor: 4.67
Species: Mouse
PMID: 31152587
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Product Promise
We promise Anti-Synaptophysin/SYP Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9409
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026