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- Table of Contents
SYP antibody (M05049-3) performs well in WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Western blot analysis of SYP using anti-SYP antibody (M05049-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat brain whole cell lysates,
Lane 3: mouse brain whole cell lysates,
Lane 4: mouse brain whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SYP antigen affinity purified polyclonal antibody (Catalog # M05049-3) at 0.5 g/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SYP at approximately 38KD. The expected band size for SYP is at 34KD.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat brain whole cell lysates,
Lane 3: mouse brain whole cell lysates,
Lane 4: mouse brain whole cell lysates,
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SYP antigen affinity purified polyclonal antibody (Catalog # M05049-3) at 0.5 g/mL overnight at 4C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SYP at approximately 38KD. The expected band size for SYP is at 34KD.
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Anti-SYP/Synaptophysin Antibody Picoband® (monoclonal, 3G12)
SYP antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Mouse | WB |
Tested Applications
SYP antibody was positively detected by WB in:
rat brain tissue, rat brain whole cell, mouse brain whole cell, mouse brain whole cell,
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-SYP/Synaptophysin Antibody Picoband® (monoclonal, 3G12)
View all SYP Antibodies
View all SYP Antibodies
Form
Lyophilized
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
3G12
Isotype
Mouse IgG2b
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Synaptophysin, identical to the related mouse and rat sequences.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
38 kDa
Background of SYP
SYP (Synaptophysin) is located on the short arm of X chromosome (Xp11.23-p11.22). The protein is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38kDa. It is present inneuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. It acts as a marker for neuroendocrine tumors, and its ubiquity at the synapse has led to the use of synaptophysin immunostaining for quantification of synapses. Also, the protein binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR).
Cite This Product
Anti-SYP/Synaptophysin Antibody Picoband® (monoclonal, 3G12) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05049-3)
Protocols
Western Blot protocol for SYP antibody
Publications
Application:
Species:
Investigation of the mechanism of tanshinone IIA to improve cognitive function via synaptic plasticity in epileptic rats
Published: 2022-12-22
Journal: PHARMACEUTICAL BIOLOGY
Application: IF,WB
Impact Factor: 3.89
Species: Rat
PMID: 36548216
Effect of Tetramethylpyrazine on Neuroplasticity after Transient Focal Cerebral Ischemia Reperfusion in Rats
Published: 2021-01-18
Journal: Evidence-based Complementary and Alternative Medicine
Application: IHC
Impact Factor: 2.63
Species: Rat
PMID: 33531914
Abnormality of synaptic vesicular associated proteins in cerebral cortex and hippocampus after microwave exposure
Published: 2009-07-14
Journal: SYNAPSE
Application: WB
Impact Factor: 2.58
Species: Rat
PMID: 19603498
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Product Promise
We promise Anti-SYP/Synaptophysin Antibody Picoband® (monoclonal, 3G12) performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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M05049-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026