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- Table of Contents
NDUFS8 antibody (A08275-2) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Flow Cytometry analysis of Hela cells using anti-NDUFS8 antibody (A08275-2).
Overlay histogram showing Hela cells stained with A08275-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFS8 Antibody (A08275-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing Hela cells stained with A08275-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFS8 Antibody (A08275-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-NDUFS8 Antibody Picoband®
NDUFS8 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
NDUFS8 antibody was positively detected by WB in:
human Hela whole cell, human HepG2 whole cell, human A549 whole cell, human MCF-7 whole cell, human CACO-2 whole cell, human PC-3 whole cell, human 293T whole cell, rat heart tissue, rat liver tissue, rat skeletal muscle tissue, mouse heart tissue, mouse liver tissue, mouse skeletal muscle tissue, mouse C2C12 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
NDUFS8 antibody was positively detected by IHC in:
human liver cancer tissue, mouse heart tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
NDUFS8 antibody was positively detected by IF in:
human liver cancer tissue
NDUFS8 antibody was positively detected by FC in:
Hela cell, 293T cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.25 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Mouse
Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x10^6 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-NDUFS8 Antibody Picoband®
View all NDUFS8 Antibodies
View all NDUFS8 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human NDUFS8 recombinant protein (Position: M1-R210).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
23 kDa
Calculated Molecular Weight
23.7 kDa
Background of NDUFS8
NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial also known as NADH-ubiquinone oxidoreductase 23 kDa subunit, Complex I-23kD (CI-23kD), or TYKY subunit is an enzyme that in humans is encoded by the NDUFS8 gene. This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome.
Cite This Product
Anti-NDUFS8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08275-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
95%
High Homology
Cow
94%
High Homology
Sheep
94%
High Homology
Dog
93%
High Homology
Rat
90%
High Homology
Mouse
90%
High Homology
Zebrafish
80%
Moderate Homology
Drosophila
73%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for NDUFS8 antibody
Immunohistochemistry (IHC) protocol for NDUFS8 antibody
Immunofluorescence (IF) protocol for NDUFS8 antibody
Flow Cytometry (FC) protocol for NDUFS8 antibody
Publications
Application:
Species:
STAT1/SLC31A1 signaling promotes diabetic retinopathy progression by mediating cuproptosis-induced M1 polarization in microglial cells
Published: 2026-01-27
Journal: LIFE SCIENCES
Application: WB
Impact Factor: 5.10
Species: Mouse
PMID: 41611203
The STAT1-SLC31A1 axis: Potential regulation of cuproptosis in diabetic retinopathy
Published: 2024-08-15
Journal: GENE
Application: WB
Impact Factor: 2.60
Species: Mouse
PMID: 39153705
SOX18 meditates the resistance of Bmi1-expressing cells to cetuximab in HNSCC
Published: 2023-05-15
Journal: ORAL DISEASES
Application: WB
Impact Factor: 3.80
Species: Human
PMID: 37184032
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Product Promise
We promise Anti-NDUFS8 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A08275-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026