Anti-NEK2 Antibody

NEK2 antibody

Boster Bio Anti-NEK2 Antibody catalog # A01606-1. Tested in WB,IHC applications. This antibody reacts with Human,Mouse.

Product Info Summary

SKU: A01606-1
Size: 100ul
Reactive Species: Human, Mouse
Host: Rabbit
Application: IHC, WB

Product Name

Anti-NEK2 Antibody

View all NEK2 Antibodies

SKU/Catalog Number

A01606-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-NEK2 Antibody catalog # A01606-1. Tested in WB,IHC applications. This antibody reacts with Human,Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-NEK2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01606-1)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human MIP-3beta

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01606-1 is reactive to NEK2 in Human, Mouse

Applications

A01606-1 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

51.763kDa

Background of NEK2

Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Component of the PSAP complex which binds RNA in a sequence-independent manner and is proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. Involved in the establishment and maintenance of epithelia cell-cell adhesion. Potential tumor suppressor for renal cell carcinoma.

Ouyang P., J. Cell Biol. 135:1027-1042(1996).
Degen W.G.J., Biochim. Biophys. Acta 1444-384-394(1999).
Shi Y., Oncogene 19:289-297(2000).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000-1:5000
IHC: 1:50-1:200

Validation Images & Assay Conditions

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Gene/Protein Information For NEK2 (Source: Uniprot.org, NCBI)

Gene Name

NEK2

Full Name

Serine/threonine-protein kinase Nek2

Weight

51.763kDa

Superfamily

protein kinase superfamily

Alternative Names

EC 2.7.11; EC 2.7.11.1; HSPK 21; HsPK21; NEK2; NEK2AHsPK21; Never in mitosis A-related kinase 2; NIMA (never in mitosis gene a)-related kinase 2; NimA-like protein kinase 1; NimA-related protein kinase 2; NLK1; NLK1serine/threonine-protein kinase Nek2 NEK2 HsPK21A, NLK1, PPP1R111, RP67, NEK2 NIMA related kinase 2 serine/threonine-protein kinase Nek2|NIMA (never in mitosis gene a)-related kinase 2|nimA-like protein kinase 1|nimA-related protein kinase 2|protein phosphatase 1, regulatory subunit 111

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NEK2, check out the NEK2 Infographic

NEK2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NEK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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Order DetailsPrice
A01606-1-100ul

100ul

$405

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