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NEK2 Antibodies
AND NEK2 ELISA kits, NEK2 Proteins
Many biological assays use antibodies to detect NEK2, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with NEK2 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop NEK2 antibodies...
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NEK2 Overview
Facts about Serine/threonine-protein kinase Nek2.
Serine/threonine-protein kinase Nek2 (NEK2)
Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins like CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes.
Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2.
Gene Information
| Human | |
|---|---|
| Gene Name: | NEK2 |
| Uniprot: | P51955 |
| Entrez: | 4751 |
Family
| Belongs to: |
|---|
| protein kinase superfamily |
Alternative Names
EC 2.7.11; EC 2.7.11.1; HSPK 21; HsPK21; NEK2; NEK2AHsPK21; Never in mitosis A-related kinase 2; NIMA (never in mitosis gene a)-related kinase 2; NimA-like protein kinase 1; NimA-related protein kinase 2; NLK1; NLK1serine/threonine-protein kinase Nek2
Molecular Weight
Mass (kDA):
51.763 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 1q32.3 |
| Sequence: | 1; NC_000001.11 (211658256..211675630, complement) |
Tissue Specificity
Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types. Isoform 1 and isoform 4 are expressed in the testis. Up- regulated in various cancer cell lines, as well as primary breast tumors.
Subcellular Localizations
[Isoform 1]: Nucleus. Nucleus, nucleolus. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome, centromere, kinetochore. Chromosome, centromere. STK3/MST2 and SAV1 are required for its targeting to the centrosome. Colocalizes with SGO1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end.; [Isoform 2]: Cytoplasm. Predominantly
Diseases
- Malignant Neoplasms
- Neoplasms
- Malignant Neoplasm Of Breast
- Mammary Neoplasms
- Carcinoma
- Cell Transformation, Neoplastic
- Aneuploidy
- Ductal Carcinoma
- Instabilities, Chromosomal
- Invasive Ductal Breast Carcinoma
- Tissue Adhesions
- Neoplasm Metastasis
- Genomic Instability
Interacting Proteins
- ANAPC4
- CDC27
- KIF24
- NEK11
Pathways
- Mitosis
- Cell Cycle
- Localization
- Chromosome Segregation
- Centrosome Separation
- Interphase
- Spindle Checkpoint
- Spindle Assembly
- Cytokinesis
- Cell Division
- Meiosis
- Metaphase
- Cell Death
PTMs
- Phosphorylation
- Cleavage
Research Areas
- Cell Cycle and Replication
- Core ESC-Like Genes
- Mitotic Regulators
- Stem Cell Markers
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/NEK2
bosterbio.com/bosterbio-gene-info-cards/NEK2
References
PMID: 7522034 by Schultz S.J., et al. Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans.
PMID: 11742531 by Hames R.S., et al. Alternative splice variants of the human centrosome kinase Nek2 exhibit distinct patterns of expression in mitosis.