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RELA antibody (M00284) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 26 Publications

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

Designing a Western blot or IHC for RELA? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.

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Anti-NF-Kappa B p65 RELA Rabbit Monoclonal Antibody

Name: Anti-NF-Kappa B p65 RELA Rabbit Monoclonal Antibody
Brand: Boster Bio
SKU: M00284
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

RELA antibody

26 Publications|

1 Review

|Submit a Review

Reactive Species	Host	Application
Human, Mouse	Rabbit	Flow Cytometry, IP, IF, IHC, ICC, WB

Tested Applications

RELA antibody was positively detected by IHC in:

human transitional cell carcinoma of bladder tissue

Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ICC

Human

Recommend

Tested

Recommend

Mouse

Recommend

Product Information

Product Name

Anti-NF-Kappa B p65 RELA Rabbit Monoclonal Antibody
View all RELA Antibodies

Form

Liquid

Uniprot

Q04206

Gene ID

5970

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

HH-18

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human NF-κB p65

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Observed Molecular Weight

65 kDa

Calculated Molecular Weight

60.2 kDa

Cite This Product

Anti-NF-Kappa B p65 RELA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00284)

Protocols

Immunohistochemistry (IHC) protocol for RELA antibody

Review Protocol

Publications

Polygonati Rhizoma Attenuates Oxidative Stress-Induced Senescence in Periodontal Ligament Stem Cells

Published: 2026-06-10

Journal: Food Science & Nutrition

Application: WB

Impact Factor: 5.00

Species: Human

PMID: 42282444

SKU	M00284
Application	Western Blot
Sample	PC-12 (rat adrenal pheochromocytoma cells, highly differentiated)
Sample Processing Description	① Normal PC12 cells (rat adrenal pheochromocytoma cells, highly differentiated)② PC12 cells are treated with β-amyloid (Aβ) to establish an in vitro Alzheimer's disease (AD) model.③ The AD in vitro model is treated with the therapeutic drug.
Other Reagents	RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer
Primary Antibody	NF-Kappa B p65 RELA Rabbit Monoclonal Antibody
Primary Incubation	1:3000, overnight at 4 ℃
Secondary Antibody	HRP-conjugated goat anti-rabbit IgG
Secondary Incubation	1:10000, 1 hour in RT
Detection	Substrate: ECL substrate; Image system: ChemiDoc MP
Results Summary	① Normal PC-12 cells (rat adrenal pheochromocytoma cells (highly differentiated)) ② PC12 cells were induced with β-amyloid protein (Aβ) to establish an in vitro model of Alzheimer's disease (AD) ③ The AD in vitro model was treated with therapeutic drugs. Experimental results showed clear target bands, and the total expression level of P65 protein was upregulated after modeling, and downregulated after drug treatment.

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Anti-NF-Kappa B p65 RELA Rabbit Monoclonal Antibody

RELA antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Polygonati Rhizoma Attenuates Oxidative Stress-Induced Senescence in Periodontal Ligament Stem Cells

TNIP3 Attenuates Colitis by Inhibiting the TAB1/NF-κB/NLRP3 Signaling Pathway in Macrophages

Mulberry leaf flavonoid extract inhibits high glucose-induced ferroptosis by regulating the PI-3K/Akt/Nrf2/GPX4 pathway in HT22 mouse hippocampal neuronal cells

Cinnamaldehyde-surfactin nano-capsulation mitigates high-fat diet/ streptozotocin-induced diabetes and modulate gut microbial dysbiosis

Investigating the Mechanism of the Fuzheng Huayu Formula in Treating Cirrhosis through Network Pharmacology, Molecular Docking, and Experimental Verification

Combining network pharmacology and experimental verification to explore the inhibitory effects of Deoxyelephantopin (DET) Against Non-Small Cell Lung Cancer (NSCLC)

Follicle-stimulating hormone peptide-conjugated liposomes in the treatment of epithelial ovarian cancer through the induction of M2-to-M1 macrophage repolarization

Rod-Shaped Microgel Scaffolds with Interconnective Pores and Oxygen-generating Functions Promote Skin Wound Healing and Alleviate Hypertrophic Scar Formation

Overactivation of NF-kB pathway can induce apoptosis by down-regulating glycolysis in human degenerative nucleus pulposus cells

Efficacy of Rhamnus utilis Decne. aqueous extract in mice with acute alcoholic liver injury and metabolomic study

Active fractions from Jingfang Baidu Powder alleviate Klebsiella-induced Pneumonia by inhibiting TLR4/Myd88-ERK signaling pathway

Structural characteristics and immunomodulation activity of a polysaccharide from purslane (Portulaca oleracea)

Total glycosides of Rhodiola rosea L. attenuate LPS-induced acute lung injury by inhibiting TLR4/NF-κB pathway

Achaete-scute complex-like 2 regulated inflammatory mechanism through Toll-like receptor 4 activating in stomach adenocarcinoma

Transmembrane p24 trafficking protein 2 regulates inflammation through the TLR4/NF-κB signaling pathway in lung adenocarcinoma

CDCA7-regulated inflammatory mechanism through TLR4/NF-κB signaling pathway in stomach adenocarcinoma

Effects of Txk‑mediated activation of NF‑κB signaling pathway on neurological deficit and oxidative stress after ischemia‑reperfusion in rats

Rhodiola rosea L. Attenuates Cigarette Smoke and Lipopolysaccharide-Induced COPD in Rats via Inflammation Inhibition and Antioxidant and Antifibrosis Pathways

The Potential Role of IL1RAP on Tumor Microenvironment-Related Inflammatory Factors in Stomach Adenocarcinoma.

COLEC12 regulates apoptosis of osteosarcoma through Toll-like receptor 4–activated inflammation

Protective effects of fisetin against myocardial ischemia/reperfusion injury

Saturated hydrogen alleviates CCl4-induced acute kidney injury via JAK2/STAT3/p65 signaling.

H19 promote calcium oxalate nephrocalcinosis-induced renal tubular epithelial cell injury via a ceRNA pathway

Kallistatin, a novel anti-angiogenesis agent, inhibits angiogenesis via inhibition of the NF-κB signaling pathway

Therapeutic effect of artemisinin on lupus nephritis mice and its mechanisms

Effect of Tongmai Decoction on expression of NF-κB in ox-LDL injured endothelial cells

Review

Have you used Anti-NF-Kappa B p65 RELA Rabbit Monoclonal Antibody?

1 Review For Anti-NF-Kappa B p65 RELA Rabbit Monoclonal Antibody

Anti-NF-κB p65 Antibody (M00284) produced a clear and specific band at the expected molecular weight with minimal background, and showed upregulated NF-κB p65 expression in Aβ-induced AD model PC-12 cells, which was decreased after drug treatment by Weste

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