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Product Info Summary
| SKU: | M00284 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-NF-Kappa B p65 RELA Rabbit Monoclonal Antibody
SKU/Catalog Number
M00284
BM3940 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-NF-Kappa B p65 RELA Rabbit Monoclonal Antibody catalog # M00284. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NF-Kappa B p65 RELA Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00284)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HH-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human NF-κB p65
Reactive Species
M00284 is reactive to RELA in Human, Mouse
Observed Molecular Weight
65 kDa
Calculated molecular weight
60.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00284 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:50
Positive Control
IHC: human transitional cell carcinoma of bladder tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:4W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:4W dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:4W dilution for 1 hour at room temperature.
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Western blot analysis of NF-κB p65 expression in HeLa cell lysate.
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Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder, using NF-κB p65 Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis of HT-1080 cells, using NF-κB p65 Antibody .
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A Western blot assay of TMED2, TLR4, NF-κB-p-p65, IL-1β, and IL-18 expression in the Con, sh-TMED2, control-shTMED2, and Spa. + sh-TMED2 groups in vitro. B Quantification of TMED2, TLR4, NF-κB-p-p65, IL-1β, and IL-18 protein expression. C Western blot assay of TMED2, TLR4, NF-κB-p-p65, IL-1β, and IL-18 expression in vivo. D Quantification of TMED2, TLR4, NF-κB-p-p65, IL-1β, and IL-18 protein expression. Protein levels were normalized to β-actin. (sh-TMED2 vs. Con group, * P < 0.05; Spa. + sh-TMED2 vs. sh-TMED2 group, # P < 0.05, n = 6 per group)
Index in PubMed under a CC BY license. PMID: 35135563
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GSEA of TMED2 with A inflammation, B TLR4, and C NF-κB in LUAD
Index in PubMed under a CC BY license. PMID: 35135563
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A Correlation analysis between ASCL2 with CNTNAP3, CLIP1, C9orf84, ARIH2, and IL1R2 in STAD. B Expression of ASCL2 in CNTNAP3, CLIP1, C9orf84, ARIH2, and IL1R2 mutant and wild-type STAD patients
Index in PubMed under a CC BY license. PMID: 36008864
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A ASCL2, TLR4, NF-κB, IL-1β, and IL-18 expressed in different groups, and B quantificated in vitro. C ASCL2, TLR4, NF-κB, IL-1β, and IL-18 expressed in different groups and D quantificated in vivo (sh-ASCL2 vs. Con, n = 6, * P < 0.05; Spa. + sh-ASCL2 vs. sh-ASCL2, n = 6,. # P < 0.05)
Index in PubMed under a CC BY license. PMID: 36008864
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A Correlation of ASCL2 with immune-associated cells in STAD. B Correlation of ASCL2 with target protein of macrophage M2, T cell follicular helper, neutrophils, dendritic cells, monocytes, T cells CD8, and macrophage M1. C , D GSEA assay of ASCL2 with gastric cancer, inflammation, and TLR4 in STAD
Index in PubMed under a CC BY license. PMID: 36008864
Specific Publications For Anti-NF-Kappa B p65 RELA Rabbit Monoclonal Antibody (M00284)
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