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Product Info Summary
| SKU: | M03142-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-GAD65/GAD2 Antibody Picoband® (monoclonal, 4E12)
SKU/Catalog Number
M03142-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GAD65/GAD2 Antibody Picoband® (monoclonal, 4E12) catalog # M03142-1. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GAD65/GAD2 Antibody Picoband® (monoclonal, 4E12) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03142-1)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
4E12
Isotype
Mouse IgG1
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human GAD65, different from the related mouse and rat sequences by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M03142-1 is reactive to GAD2 in Mouse, Rat
Observed Molecular Weight
65 kDa
Calculated molecular weight
65.4 kDa
Background of GAD2
Glutamate decarboxylase 2, also known as GAD65, is an enzyme that in humans is encoded by the GAD2 gene. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03142-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Western blot analysis of GAD65/GAD2 using anti-GAD65/GAD2 antibody (M03142-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-GAD65/GAD2 antigen affinity purified monoclonal antibody (Catalog # M03142-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GAD65/GAD2 at approximately 65KD. The expected band size for GAD65/GAD2 is at 65KD.
Specific Publications For Anti-GAD65/GAD2 Antibody Picoband® (monoclonal, 4E12) (M03142-1)
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