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RELA antibody (PA1669) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 96 Publications

pa1669 rela primary antibodies ihc testing 2

IHC analysis of NF-kB p65/RELA using anti-NF-kB p65/RELA antibody (PA1669).
NF-kB p65/RELA was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-NF-kB p65/RELA Antibody (PA1669) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Anti-NF-kB p65/RELA Antibody Picoband®

RELA antibody

96 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	IF, IHC, ICC, WB

Tested Applications

RELA antibody was positively detected by WB in:

human HT1080 whole cell lysates, human Hela whole cell lysates, human Raji whole cell lysates, human HepG2 whole cell lysates, rat PC-12 whole cell lysates, rat C6 whole cell lysates, mouse NIH/3T3 whole cell lysates, mouse Ana-1 whole cell lysates

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

RELA antibody was positively detected by IHC in:

human breast cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

RELA antibody was positively detected by ICC/IF in:

U20S cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Rat

Tested

Mouse

Tested

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Monkey

Predicted

Product Information

Product Name

Anti-NF-kB p65/RELA Antibody Picoband®
View all RELA Antibodies

Form

Lyophilized

Uniprot

Q04206

Gene ID

5970

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human NF-kB p65, identical to the related rat and mouse sequences.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

65-70 kDa

Calculated Molecular Weight

60.2 kDa

Background of RELA

RELA (V-REL AVIAN RETICULOENDOTHELIOSIS VIRAL ONCOGENE HOMOLOG A), also called NFKB3 or NFKB, p65 SUBUNIT. NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins, which inactivate NFKB by trapping it in the cytoplasm. The p65 (RELA) heterodimer is the most abundant form of NFKB. And the RELA gene is located on 11q13.1. RELA is a nonhistone substrate of HDAC3 and that IKBA-dependent nuclear export of the HDAC3-deacetylated RELA replenishes the depleted cytoplasmic pool of latent NFKB-IKBA complexes for subsequent NFKB responses. RELA nucleocytoplasmic redistribution coincided with export of PPARG, and immunoprecipitation analysis indicated that PPARG-RELA association was dependent on the PPARG C-terminal ligand-binding domain. IKK-dependent phosphorylation of RELA on ser468 enhanced binding of GCN5 to RELA and RELA ubiquitination.

Cite This Product

Anti-NF-kB p65/RELA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1669)

Protocols

Western Blot protocol for RELA antibody

Review Protocol

Immunohistochemistry (IHC) protocol for RELA antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for RELA antibody

Review Protocol

Publications

SEM1 Downregulates HSPA8 to Suppress TLR4/MyD88/NF-κB Signaling and Alleviate Myocardial I/R Injury

Published: 2026-07-27

Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Application: WB

Impact Factor: 5.60

Species: Rat

PMID: 42589368

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Anti-NF-kB p65/RELA Antibody Picoband®

RELA antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

SEM1 Downregulates HSPA8 to Suppress TLR4/MyD88/NF-κB Signaling and Alleviate Myocardial I/R Injury

DPPA inhibits melanoma by targeting angiogenesis through activating autocrine IFN-γ-CXCL9/10/11-CXCR3 axis in vascular endothelial cells

Skin bacteriota ameliorates androgenetic alopecia via harmonizing skin immuno inflammatory balance

Gu-Jin-Xiao-Ji Decoction potentiates anti-PD-1 immunotherapy in lung cancer by inhibiting the PI3K/AKT/NF-κB/PD-L1 axis

Repurposing Lamivudine: A novel therapeutic strategy for ischemic stroke targeting the glycolytic enzyme Pgk1

Red nucleus IL-15 facilitates the development of neuropathic pain in male rats by inducing inflammatory factors: implying the involvement of NF-κB and p38 MAPK

Tanshinone IIA and hydroxy safflower yellow A reduce cerebral injury via TLR4/NF-κB pathway in rats

Sea buckthorn regulates PXR/CAR/NF-κB signaling and restores CYP2C metabolic function in BCG-induced hepatitis

Astragaloside IV suppression of chronic atrophic gastritis by upregulating PAR-1 in vitro and in vivo

Rod-Shaped Microgel Scaffolds with Interconnective Pores and Oxygen-generating Functions Promote Skin Wound Healing and Alleviate Hypertrophic Scar Formation

Semaglutide Alleviates Ovary Inflammation via the AMPK/SIRT1/NF‑κB Signaling Pathway in Polycystic Ovary Syndrome Mice

Overactivation of NF-kB pathway can induce apoptosis by down-regulating glycolysis in human degenerative nucleus pulposus cells

The Role of Grifola frondosa Polysaccharide in Preventing Skeletal Muscle Atrophy in Type 2 Diabetes Mellitus

Antimicrobial peptide-modified pH-sensitive micelles against pneumonia caused by methicillin-resistant Staphylococcus aureus

Efficacy of Rhamnus utilis Decne. aqueous extract in mice with acute alcoholic liver injury and metabolomic study

Active fractions from Jingfang Baidu Powder alleviate Klebsiella-induced Pneumonia by inhibiting TLR4/Myd88-ERK signaling pathway

Three Artemisia pollens trigger the onset of allergic rhinitis via TLR4/MyD88 signaling pathway

Baicalin-2-ethoxyethyl ester alleviates gentamicin-induced acute kidney injury via NF-κB signaling pathway

Lead exposure induces neuronal apoptosis via NFκB p65/RBBP4/Survivin signaling pathway

Sirtuin1 inhibits calcium oxalate crystal-induced kidney injury by regulating TLR4 signaling and macrophage-mediated inflammatory activation

Gut microbiota contribution to selenium deficiency-induced gut–liver inflammation

Deletion of Brucella transcriptional regulator GntR10 regulated the expression of quorum sensing system and type IV secretion system effectors, which affected the activation of NF-κB

Sirt4 deficiency promotes the development of atherosclerosis by activating the NF-κB/IκB/CXCL2/3 pathway

HMGB1 promotes Ox-LDL-induced endothelial cell damage by inhibiting PI3K/Akt signaling pathway

Short-Chain Fatty Acids Weaken Ox-LDL-Induced Cell Inflammatory Injury by Inhibiting the NLRP3/Caspase-1 Pathway and Affecting Cellular Metabolism in THP-1 Cells

Characterization of SHARPIN knockout Syrian hamsters developed using CRISPR/Cas9 system

Adipose tissue-derived mesenchymal stem cells attenuate lung inflammation and fibrosis in the bleomycin-induced pulmonary fibrosis rat model via caveolin-1/NF-kB signaling axis

Folic acid ameliorates N-methyl-N′-nitro-N-nitrosoguanidine-induced esophageal inflammation via modulation of the NF-κB pathway

Using extracellular vesicles derived from human umbilical cord mesenchymal stem cells for a topical coating promotes oral mucositis healing in rats

Surfactin-oleogel with therapeutic potential for inflammatory acne vulgaris induced by Propionibacterium acnes

Red nucleus IL-33 facilitates the early development of mononeuropathic pain in male rats by inducing TNF-α through activating ERK, p38 MAPK, and JAK2/STAT3

Glucagon-like peptide 1 treatment reverses vascular remodelling by downregulating matrix metalloproteinase 1 expression through inhibition of the ERK1/2/NF-κB signalling pathway

Combination of cyclophosphamide and shengbai decoction has synergistic effect against melanoma

NF-κB-mediated regulation of rat CYP2E1 by two independent signaling pathways

Tumor-associated macrophages secrete CC-chemokine ligand 2 and induce tamoxifen resistance by activating PI3K/Akt/mTOR in breast cancer

Paeonol antagonizes oncogenesis of osteosarcoma by inhibiting the function of TLR4/MAPK/NF-κB pathway

Rhynchophylline attenuates migraine in trigeminal nucleus caudalis in nitroglycerin-induced rat model by inhibiting MAPK/NF-кB signaling

Partial liquid ventilation–induced mild hypothermia improves the lung function and alleviates the inflammatory response during acute respiratory distress syndrome in canines

Possible Mechanism of the Anti-inflammatory Activity of Ruscogenin: Role of Intercellular Adhesion Molecule-1 and Nuclear Factor-κB

PC-3-Derived Exosomes Inhibit Osteoclast Differentiation by Downregulating miR-214 and Blocking NF-B Signaling Pathway

Total flavonoids, extracted from Polygonum knotweed L, exert beneficial hepatoprotection against liver injury

Probiotic Lactobacillus rhamnosus modulates the gut microbiome composition attenuates preneoplastic colorectal Aberrant crypt foci

Andrographolide prevents human nucleus pulposus cells against degeneration by inhibiting the NF-κB pathway

Red nucleus interleukin-1β evokes tactile allodynia through activation of JAK/STAT3 and JNK signaling pathways

Platycodin D protects acetaminophen-induced hepatotoxicity by inhibiting hepatocyte MAPK pathway and apoptosis in C57BL/6J mice

Protective effect of isoliquiritigenin against cerebral injury in septic mice via attenuation of NF-κB

Hepatoprotective effects exerted by Poria Cocos polysaccharides against acetaminophen-induced liver injury in mice

Shunaoxin dropping pill, a Chinese herb compound preparation, attenuates memory impairment in D-galactose-induced aging mice

Isolation, purification, structural characterization and immunostimulatory activity of water-soluble polysaccharides from Lepidium meyenii

The CXCL12–CXCR4 axis promotes migration, invasiveness, and EMT in human papillary thyroid carcinoma B-CPAP cells via NF-κB signaling

Prophylactic effects of triptolide on colon cancer development in azoxymethane/dextran sulfate sodiuminduced mouse model

Sophocarpine attenuates wear particle-induced implant loosening by inhibiting osteoclastogenesis and bone resorption via suppression of the NF-κB signalling pathway in a rat model

Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats

Unfractionated heparin protects the protein C system against lipopolysaccharide‑induced damage in vivo and in vitro

Individual and combined use of ginsenoside F2 and cyanidin-3-O-glucoside attenuates H2O2-induced apoptosis in HEK-293 cells via the NF-κB pathway

Epigallocatechin‑3‑gallate attenuates neointimal hyperplasia in a rat model of carotid artery injury by inhibition of high mobility group box 1 expression

MicroRNA-155 regulates arsenite-induced malignant transformation by targeting Nrf2-mediated oxidative damage in human bronchial epithelial cells

Design and discovery of novel thiazole derivatives as potential MMP inhibitors to protect against acute lung injury in sepsis rats via attenuation of inflammation and apoptotic oxidative stress

Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats Corrigendum in /10.3892/mmr.2018.8625

Silencing of Y-box binding protein-1 by RNA interference inhibits proliferation, invasion, and metastasis, and enhances sensitivity to cisplatin through NF-κB signaling pathway in human neuroblastoma SH-SY5Y cells

The vascular protective effects of Anoectochilus roxburghii polysaccharose under high glucose conditions

PD-L1 is upregulated by EBV-driven LMP1 through NF-κB pathway and correlates with poor prognosis in natural killer/T-cell lymphoma

Prognostic value of toll-like receptor 4 and nuclear factor-κBp65 in oral squamous cell carcinoma patients

Lactobacillus rhamnosus induced epithelial cell apoptosis, ameliorates inflammation and prevents colon cancer development in an animal model

Zisheng Shenqi decoction ameliorates monosodium urate crystal-induced gouty arthritis in rats through anti-inflammatory and anti-oxidative effects

Indirubin ameliorates dextran sulfate sodium-induced ulcerative colitis in mice through the inhibition of inflammation and the induction of Foxp3-expressing regulatory T cells

Gypenoside Protects Cardiomyocytes against Ischemia-Reperfusion Injury via the Inhibition of Mitogen-Activated Protein Kinase Mediated Nuclear Factor Kappa B Pathway In Vitro and In Vivo