Anti-NF-kB p65/RELA Antibody
Rabbit IgG polyclonal antibody for Transcription factor p65(RELA) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat. Cited in 49 publication(s).
Anti-NF-kB p65/RELA Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Applications:||IHC, ICC, WB|
|Product Name||Anti-NF-kB p65/RELA Antibody
See all RELA primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for NFkB p65/RELA detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. NFkB p65/RELA information: Molecular Weight: 60219 MW; Subcellular Localization: Nucleus. Cytoplasm. Colocalized with DDX1 in the nucleus upon TNF-alpha induction (By similarity). Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalizes with GFI1 in the nucleus after LPS stimulation.|
|Cite This Product||Anti-NF-kB p65/RELA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1669)|
|Similar Products From
|Anti-NF-kB p65/RELA Antibody may replace the following items: sc 81622.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Applications||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human NF-kB p65(143-158aa VPIEEQRGDYDLNAVR), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For RELA (Source: Uniprot.org, NCBI)
|NCBI Gene Id||5970|
|Species Of This Entry||Human|
|Protein Name||Transcription factor p65|
|Alternative Names||RELA|NF kB p65; NFkB p65; NF-kB p65; p65; rela p65; RelA; MGC131774; nf kb p65; NFkB p65; NF-kB p65; NFKB3; NFKB3v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor ofkappa light polypeptide gene enhancer in B-cells 3 (p65)); Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3transcription factor p65; p65 flow cytometry; p65 immunohistochemistry; p65; p65RelA; rela p65; RelA; v-rel reticuloendotheliosis viral oncogene homolog A (avian); v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappalight po|
|Post Tranlational Modifications||Phosphorylation; Acetylation; Cleavage; Ubiquitination; Deacetylation; Methylation; Dephosphorylation; Sumoylation; Reduction; Phosphorothioation; Oxidation; Glutathionylation; Demethylation; Nitration; Glycosylation; Nitrosylation; Formylation; Carboxylation; Amidation; Ribosylation|
|Subcellular Localization||Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B) (PubMed:1493333). Colocalized with DDX1 in the nucleus upon TNF-alpha induction (PubMed:19058135). Colocalizes with GFI1 in the nucleus after LPS stimulation (PubMed:20547752). Translocation to the nucleus is impaired in L.monocytogenes infection (PubMed:20855622).|
|Gene Location||11q13.1, on Chromasome 11, gene sequence: NC_000011.10 (65653596..65662972, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For RELA (Source: Uniprot.org, NCBI)
|Protein Function||NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (PubMed:15790681).|
|Research Areas||Apoptosis; Cancer; Cell Biology; Immune System Diseases; Immunology; Innate Immunity; Neurodegeneration; Neuroscience; Signal Transduction; Transcription Factors and Regulators|
|Related Diseases||Inflammation; Malignant Neoplasms; Neoplasms; Tissue Adhesions; Cell Transformation, Neoplastic; Carcinoma; Inflammatory Response; Leukemia; Neoplasm Metastasis; Mammary Neoplasms; Malignant Neoplasm Of Breast; Cell Invasion; Pneumonia; Lymphoma; Hiv Infections; Atherosclerosis; Immunologic Deficiency Syndromes; Malignant Paraganglionic Neoplasm; Diabetes Mellitus; Adenocarcinoma|
|Related Pathways||Cell Death; Localization; Pathogenesis; Inflammatory Response; Cell Proliferation; Secretion; Immune Response; Cell Cycle; Cell Growth; Virulence; Translation; Transport; Cytokine Production; Cell Adhesion; Cell Activation; Rna Interference; Angiogenesis; Oncogenesis; Induction Of Apoptosis; Dna Replication|
|Background||RELA(V-REL AVIAN RETICULOENDOTHELIOSIS VIRAL ONCOGENE HOMOLOG A), also called NFKB3 or NFKB, p65 SUBUNIT. NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins, which inactivate NFKB by trapping it in the cytoplasm. The p65(RELA) heterodimer is the most abundant form of NFKB. And the RELA gene is located on 11q13.1. RELA is a nonhistone substrate of HDAC3 and that IKBA-dependent nuclear export of the HDAC3-deacetylated RELA replenishes the depleted cytoplasmic pool of latent NFKB-IKBA complexes for subsequent NFKB responses. RELA nucleocytoplasmic redistribution coincided with export of PPARG, and immunoprecipitation analysis indicated that PPARG-RELA association was dependent on the PPARG C-terminal ligand-binding domain. IKK-dependent phosphorylation of RELA on ser468 enhanced binding of GCN5 to RELA and RELA ubiquitination.|
|Scientific References||PMID: 2006423 by Ruben S.M., et al. Isolation of a rel-related human cDNA that potentially encodes the 65-kD subunit of NF-kappa B.|
PMID: 8281153 by Deloukas P., et al. Genomic organization of the gene encoding the p65 subunit of NF-kappa B: multiple variants of the p65 protein may be generated by alternative splicing.
Our Boster Quality Guarantee for Anti-NF-kB p65/RELA Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Testis Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: A431 Cell Lysate
Lane 4: JURKAT Cell Lysate
IHC(P): Human Mammary Cancer Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 49
|Fan Z, Yao J, Li Y, Hu X, Shao H, Tian X. Int J Clin Exp Pathol. 2015 Apr 1;8(4):3451-9. Ecollection 2015. Anti-Inflammatory And Antioxidant Effects Of Curcumin On Acute Lung Injury In A Rodent Model Of Intestinal Ischemia Reperfusion By Inhibitin...||PubMed ID 26097529|
|Li B, Chu X, Gao M, Li W. Acta Biochim Biophys Sin (Shanghai). 2010 Jan;42(1):80-9. Apoptotic Mechanism Of Mcf-7 Breast Cells In Vivo And In Vitro Induced By Photodynamic Therapy With C-Phycocyanin.||PubMed ID 20043050|
|Kan S, Zhou H, Jin C, Yang H. Int J Clin Exp Med. 2015 Mar 15;8(3):3258-70. Ecollection 2015. Effects Of Pdtc On Nf-??b Expression And Apoptosis In Rats With Severe Acute Pancreatitis-Associated Lung Injury.||PubMed ID 26064215|
|Yuan W, Yang D, Sun X, Liu W, Wang L, Li X, Man X, Fu Q. Int J Clin Exp Pathol. 2014 Aug 15;7(9):5772-81. Ecollection 2014. Effects Of Hydroxysafflor Yellow A On Proliferation And Collagen Synthesis Of Rat Vascular Adventitial Fibroblasts Induced ...||PubMed ID 25337218|
|Liu Mw, Su Mx, Zhang W, Wang Yh, Qin Lf, Liu X, Tian Ml, Qian Cy. Mol Med Rep. 2015 May;11(5):3308-16. Doi: 10.3892/Mmr.2015.3146. Epub 2015 Jan 7. Effect Of Melilotus Suaveolens Extract On Pulmonary Microvascular Permeability By Downregulating Va...||PubMed ID 25571852|
|Li Y, Chen Y, Zhang J, Zhu Jf, Liu Zj, Liang Sy, Sun K, Liao Wy, Gong Jp. Am J Surg. 2010 Jan;199(1):35-42. Doi: 10.1016/J.Amjsurg.2008.11.039. Protective Effect Of Glutamine-Enriched Early Enteral Nutrition On Intestinal Mucosal Barrier Injury Af...||PubMed ID 20103064|
|Wang J, Du Jr, Wang Y, Kuang X, Wang Cy. Acta Pharmacol Sin. 2010 Jul;31(7):791-7. Doi: 10.1038/Aps.2010.71. Epub 2010 Jun 28. Z-Ligustilide Attenuates Lipopolysaccharide-Induced Proinflammatory Response Via Inhibiting Nf-Kappab Pathway In Primary...||PubMed ID 20581853|
|Zhang Qq, Ding Y, Lei Y, Qi Cl, He Xd, Lan T, Li Jc, Gong P, Yang X, Geng Jg, Wang Lj. Int J Biol Sci. 2014 Mar 21;10(4):404-14. Doi: 10.7150/Ijbs.7723. Ecollection 2014. Andrographolide Suppress Tumor Growth By Inhibiting Tlr4/Nf-??b Signaling Ac...||PubMed ID 24719558|
|Zhao Yj, Wang H, Liu X, Sun M, Kazuhiro H. Mol Med Rep. 2012 Oct;6(4):739-44. Doi: 10.3892/Mmr.2012.1007. Epub 2012 Jul 26. Protective Effects Of Glutamine In A Rat Model Of Endotoxemia.||PubMed ID 22842774|
|Leng Am, Liu T, Yang J, Cui Jf, Li Xh, Zhu Yn, Xiong T, Zhang G, Chen Y. Cell Biol Int. 2012 Oct 1;36(10):893-9. Doi: 10.1042/Cbi20110473. The Apoptotic Effect And Associated Signalling Of Hsp90 Inhibitor 17-Dmag In Hepatocellular Carcinoma Cells.||PubMed ID 22694478|
|Ren Zh, Tong Yh, Xu W, Ma J, Chen Y. Phytomedicine. 2010 Mar;17(3-4):212-8. Doi: 10.1016/J.Phymed.2009.08.010. Epub 2009 Oct 2. Tanshinone Ii A Attenuates Inflammatory Responses Of Rats With Myocardial Infarction By Reducing Mcp-1 Expression.||PubMed ID 19800776|
|Sun, G., Yang, W., Zhang, Y., & Zhao, M. (2017). Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats. Experimental Animals. Advance online publication. doi: 10.1538/expanim.16-0102||PubMed ID 28637971|
|Zhou HJ, Li H, Shi MQ, Mao XN, Liu DL, Chang YR, Gan YM, Kuang X, Du JR. Front Pharmacol. 2018 Jan 18;8:950. doi: 10.3389/fphar.2017.00950. eCollection 2017. Protective Effect of Klotho against Ischemic Brain Injury Is Associated with Inhibition o...||PubMed ID 29403373|
|Hu LH, Fan YJ, Li Q, Guan JM, Qu B, Pei FH, Liu BR. Mol Med Rep. 2017 Jun;15(6):4093-4099. doi: 10.3892/mmr.2017.6524. Epub 2017 Apr 27. Bortezomib protects against dextran sulfate sodium-induced ulcerative colitis in mice||PubMed ID 28487944|
|Effect of Aspirin on the Expression of Hepatocyte NF-?B and Serum TNF-? in Streptozotocin-Induced Type 2 Diabetic Rats||PubMed ID 21655062|
|Dual effects of ouabain on the regulation of proliferation and apoptosis in human umbilical vein endothelial cells: involvement of Na+-K+-ATPase ?-subunits and NF-?B||PubMed ID 24995076|
|Intra-Spinal Bone Marrow Mononuclear Cells Transplantation Inhibits the Expression of Nuclear Factor-?B in Acute Transection Spinal Cord Injury in Rats||PubMed ID 25535513|
|Adenovirus-mediated transfection with glucose transporter 3 suppresses PC12 cell apoptosis following ischemic injury||PubMed ID 25657665|
|Protective Effects of Hydrogen-Rich Saline on Rats with Smoke Inhalation Injury||PubMed ID 26090070|
|Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-?B and TGF-?1/Smad-2/-3 pathways||PubMed ID 26577463|
|Fenofibrate, a PPAR? agonist, protect proximal tubular cells from albumin-bound fatty acids induced apoptosis via the activation of NF-kB||PubMed ID 26617775|
|BMSCs Interactions with Adventitial Fibroblasts Display Smooth Muscle Cell Lineage Potential in Differentiation and Migration That Contributes to Neointimal Formation||PubMed ID 26880952|
|Glycyrrhizic Acid Attenuates Sepsis-Induced Acute Kidney Injury by Inhibiting NF-?B Signaling Pathway||PubMed ID 26904148|
|Overexpression of Livin promotes migration and invasion of colorectal cancer cells by induction of epithelial-mesenchymal transition via NF-?B activation||PubMed ID 27013894|
|Effects of resveratrol and genistein on nuclear factor??B, tumor necrosis factor?? and matrix metalloproteinase?9 in patients with chronic obstructive pulmonary disease||PubMed ID 27035424|
|Valproate ameliorates nitroglycerin-induced migraine in trigeminal nucleus caudalis in rats through inhibition of NF-?B||PubMed ID 27150105|
|Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP(+))-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling pathway||PubMed ID 27164829|
|Silencing of carboxypeptidase E inhibits cell proliferation, tumorigenicity, and metastasis of osteosarcoma cells||PubMed ID 27274275|
|Gypenoside Protects Cardiomyocytes against Ischemia-Reperfusion Injury via the Inhibition of Mitogen-Activated Protein Kinase Mediated Nuclear Factor Kappa B Pathway In Vitro and In Vivo||PubMed ID 27313532|
|Hydrogen Sulfide Mitigates Kidney Injury in High Fat Diet-Induced Obese Mice||PubMed ID 27413418|
|PD-L1 is upregulated by EBV-driven LMP1 through NF-?B pathway and correlates with poor prognosis in natural killer/T-cell lymphoma||PubMed ID 27737703|
|Oligomannurarate sulfate blocks tumor growth by inhibiting NF-?B activation||PubMed ID 20154712|
|Histone deacetylase inhibitor, butyrate, attenuates lipopolysaccharide-induced acute lung injury in mice||PubMed ID 20302656|
|Ozone preconditioning and exposure to ketamine attenuates hepatic inflammation in septic rats||PubMed ID 23185204|
|MiR199b Suppresses Expression of Hypoxia-Inducible Factor 1? (HIF-1?) in Prostate Cancer Cells||PubMed ID 23594994|
|Molecular responses of radiation-induced liver damage in rats||PubMed ID 25483171|
|microRNA-146a inhibits cancer metastasis by downregulating VEGF through dual pathways in hepatocellular carcinoma||PubMed ID 25608619|
|Down-regulation of HMGB1 expression by shRNA constructs inhibits the bioactivity of urothelial carcinoma cell lines via the NF-?B pathway||PubMed ID 26239046|
|CD11b deficiency suppresses intestinal tumor growth by reducing myeloid cell recruitment||PubMed ID 26526388|
|Simvastatin ameliorates renal lipidosis through the suppression of renal CXCL16 expression in mice with adriamycin-induced nephropathy.||PubMed ID 26884839|
|Zisheng Shenqi decoction ameliorates monosodium urate crystal-induced gouty arthritis in rats through anti-inflammatory and anti-oxidative effects||PubMed ID 27432278|
|Puerarin Improves Diabetic Aorta Injury by Inhibiting NADPH Oxidase-Derived Oxidative Stress in STZ-Induced Diabetic Rats||PubMed ID 27641696|
|Effect of stromal cell-derived factor-1 on myocardial apoptosis and cardiac function recovery in rats with acute myocardial infarction||PubMed ID 27882150|
|Unfractionated heparin protects the protein C system against lipopolysaccharide-induced damage in vivo and in vitro||PubMed ID 29285085|
|Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction||PubMed ID 29390038|
|Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats||PubMed ID 29484403|
|Src Promotes Metastasis of Human Non-Small Cell Lung Cancer Cells through Fn14-Mediated NF-?B Signaling||PubMed ID 29500337|
|N-acetylcysteine reduces oxidative stress, nuclear factor-?B activity and cardiomyocyte apoptosis in heart failure||PubMed ID 24889421|
|Nuclear factor-kappaB activation on the reactive oxygen species in acute necrotizing pancreatitic rats||PubMed ID 16015706|
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