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Product Info Summary
| SKU: | M00110-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-SP1 Antibody
SKU/Catalog Number
M00110-1
Size
80 µl
Description
Boster Bio Anti-SP1 Antibody (Catalog # M00110-1). Tested in IF, Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-SP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00110-1)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1326CT463.109.176
Isotype
IgG1,κ
Immunogen
This SP1 antibody is generated from a mouse immunized with a recombination protein from the human SP1.
Reactive Species
M00110-1 is reactive to SP1 in Human
Calculated molecular weight
80.7 kDa
Background of SP1
Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR- alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00110-1 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:1000
FC: 1:25
Validation Images & Assay Conditions
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Fluorescent image of Hela cells stained with SP1 Antibody. M00110-1 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Western blot analysis of lysates from Hela, Ramos cell line (from left to right), using SP1 Antibody (Cat. #M00110-1). M00110-1 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.
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Flow cytometric analysis of Hela cells using SP1 Antibody (green, Cat#M00110-1) compared to an isotype control of mouse IgG1 (blue). M00110-1 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Specific Publications For Anti-SP1 Antibody (M00110-1)
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