Anti-SP1 Antibody

Product Name

Anti-SP1 Antibody

View all SP1 Antibodies

SKU/Catalog Number

M00110-1

Size

80 µl

Description

Boster Bio Anti-SP1 Antibody (Catalog # M00110-1). Tested in IF, Flow Cytometry, WB application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-SP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00110-1)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

1326CT463.109.176

Isotype

IgG1,κ

Immunogen

This SP1 antibody is generated from a mouse immunized with a recombination protein from the human SP1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00110-1 is reactive to SP1 in Human

Applications

M00110-1 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

80.693kDa

Background of SP1

Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR- alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. In complex with ATF7IP, maintains telomerase activity in cancer cells by inducing TERT and TERC gene expression.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:25
WB: 1:1000
FC: 1:25

Validation Images & Assay Conditions

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Fluorescent image of Hela cells stained with SP1 Antibody. M00110-1 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).

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Western blot analysis of lysates from Hela, Ramos cell line (from left to right), using SP1 Antibody (Cat. #M00110-1). M00110-1 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

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Flow cytometric analysis of Hela cells using SP1 Antibody (green, Cat#M00110-1) compared to an isotype control of mouse IgG1 (blue). M00110-1 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.

Gene/Protein Information For SP1 (Source: Uniprot.org, NCBI)

Alternative Names

Sp1 transcription factor; specificity protein 1; transcription factor Sp1; TSFP1 SP1 Sp1 transcription factor transcription factor Sp1|specificity protein 1

For more info on SP1, check out the SP1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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