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Product Info Summary
| SKU: | A00284S529 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-NFkB-p65 (phospho-S529) RELA Antibody
SKU/Catalog Number
A00284S529
Size
100ul
Form
Liquid
Description
Boster Bio Anti-NFkB-p65 (phospho-S529) RELA Antibody catalog # A00284S529. Tested in WB,IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-NFkB-p65 (phospho-S529) RELA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00284S529)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phosphopeptide derived from human NFkB-p65 around the phosphorylation site of Serine 529.
Reactive Species
A00284S529 is reactive to RELA in Human
Calculated molecular weight
60.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00284S529 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
IHC: 1:100-1:200
Validation Images & Assay Conditions
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Western blot analysis of NF-kappaB p65 (pS529) expression in A2780 (A), A549 (B) whole cell lysates.
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Immunohistochemical analysis of NF-kappaB p65 (pS529) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunoprecipitation of Jurkat(Calyculin A 50nM, 30min) cell lysates using NFkB-p65 (phospho-S529) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4) .Lane 1 is 30% input. The western blot was probed using NFkB-p65 (phospho-S529) pAb.
Specific Publications For Anti-NFkB-p65 (phospho-S529) RELA Antibody (A00284S529)
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