Anti-Notch1 Monoclonal Antibody

NOTCH1 antibody

Boster Bio Anti-Notch1 Monoclonal Antibody catalog # M00033. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: M00033
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-Notch1 Monoclonal Antibody

View all NOTCH1 Antibodies

SKU/Catalog Number

M00033

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Notch1 Monoclonal Antibody catalog # M00033. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Notch1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00033)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

BGA-14

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Notch1 Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00033 is reactive to NOTCH1 in Human, Mouse, Rat

Applications

M00033 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

110 kDa

Calculated molecular weight

272.505kDa

Background of NOTCH1

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:3000
IHC 1:50-1:200
ICC/IF 1:100-1:500
FC 1:200-1:500

Validation Images & Assay Conditions

Gene/Protein Information For NOTCH1 (Source: Uniprot.org, NCBI)

Gene Name

NOTCH1

Full Name

Neurogenic locus notch homolog protein 1

Weight

272.505kDa

Superfamily

NOTCH family

Alternative Names

EC 2.1.2.11; EC 3.4.21.68; hN1; Notch (Drosophila) homolog 1 (translocation-associated); notch 1Notch homolog 1, translocation-associated (Drosophila); Notch homolog 1, translocation-associated; Notch1; Notch-1; TAN1; TAN1neurogenic locus notch homolog protein 1; Translocation-associated notch protein TAN-1 NOTCH1 AOS5, AOVD1, TAN1, hN1 notch receptor 1 neurogenic locus notch homolog protein 1|Notch homolog 1, translocation-associated|notch 1|translocation-associated notch protein TAN-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NOTCH1, check out the NOTCH1 Infographic

NOTCH1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NOTCH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00033 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Involvement of Notch2 in all%u2011trans retinoic acid%u2011induced inhibition of mouse embryonic palate mesenchymal cell proliferation

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16 Customer Q&As for Anti-Notch1 Monoclonal Antibody

Question

We are interested in using your anti-Notch1 Monoclonal antibody for arterial endothelial cell differentiation studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-04-15

Answer

Thank you for your inquiry. This M00033 anti-Notch1 Monoclonal antibody is tested on hela cells. It is guaranteed to work for IF, IHC, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-04-15

Question

I have attached the WB image, lot number and protocol we used for brain using anti-Notch1 Monoclonal antibody M00033. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-03-13

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-13

Question

My question regarding product M00033, anti-Notch1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-02-11

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00033 anti-Notch1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-02-11

Question

Would M00033 anti-Notch1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-10

Answer

As indicated on the product datasheet, M00033 anti-Notch1 Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-10

Question

Is this M00033 anti-Notch1 Monoclonal antibody reactive to the isotypes of NOTCH1?

Verified Customer

Verified customer

Asked: 2019-11-07

Answer

The immunogen of M00033 anti-Notch1 Monoclonal antibody is A synthesized peptide derived from human Notch1 Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-07

Question

Does anti-Notch1 Monoclonal antibody M00033 work for IHC with brain?

Verified Customer

Verified customer

Asked: 2019-09-20

Answer

According to the expression profile of brain, NOTCH1 is highly expressed in brain. So, it is likely that anti-Notch1 Monoclonal antibody M00033 will work for IHC with brain.

Boster Scientific Support

Answered: 2019-09-20

Question

We are currently using anti-Notch1 Monoclonal antibody M00033 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-30

Answer

The anti-Notch1 Monoclonal antibody (M00033) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-30

Question

Do you have a BSA free version of anti-Notch1 Monoclonal antibody M00033 available?

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Notch1 Monoclonal antibody M00033 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-07-25

Question

I see that the anti-Notch1 Monoclonal antibody M00033 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-06-11

Question

I was wanting to use your anti-Notch1 Monoclonal antibody for IHC for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?

Verified Customer

Verified customer

Asked: 2018-12-25

Answer

You can see on the product datasheet, M00033 anti-Notch1 Monoclonal antibody has been tested for IF, IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-12-25

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Notch1 Monoclonal antibody M00033. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-07-31

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-07-31

Question

Is a blocking peptide available for product anti-Notch1 Monoclonal antibody (M00033)?

Verified Customer

Verified customer

Asked: 2018-05-16

Answer

We do provide the blocking peptide for product anti-Notch1 Monoclonal antibody (M00033). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-05-16

Question

We want to test anti-Notch1 Monoclonal antibody M00033 on human brain for research purposes, then I may be interested in using anti-Notch1 Monoclonal antibody M00033 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

T. Singh

Verified customer

Asked: 2016-11-25

Answer

The products we sell, including anti-Notch1 Monoclonal antibody M00033, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2016-11-25

Question

We have been able to see staining in mouse aortic endothelium. What should we do? Is anti-Notch1 Monoclonal antibody supposed to stain aortic endothelium positively?

A. Zhao

Verified customer

Asked: 2015-10-16

Answer

From what I have seen in literature aortic endothelium does express NOTCH1. From what I have seen in Uniprot.org, NOTCH1 is expressed in visceral pleura, brain, aortic endothelium, cervix carcinoma, among other tissues. Regarding which tissues have NOTCH1 expression, here are a few articles citing expression in various tissues:
Aortic endothelium, Pubmed ID: 17573339
Brain, Pubmed ID: 15164053
Cervix carcinoma, Pubmed ID: 18669648

Boster Scientific Support

Answered: 2015-10-16

Question

We were content with the WB result of your anti-Notch1 Monoclonal antibody. However we have seen positive staining in brain notch 1 intracellular domain: nucleus using this antibody. Is that expected? Could you tell me where is NOTCH1 supposed to be expressed?

J. Yang

Verified customer

Asked: 2014-07-24

Answer

From what I have seen in literature, brain does express NOTCH1. Generally NOTCH1 expresses in cell membrane, notch 1 intracellular domain: nucleus. Regarding which tissues have NOTCH1 expression, here are a few articles citing expression in various tissues:
Aortic endothelium, Pubmed ID: 17573339
Brain, Pubmed ID: 15164053
Cervix carcinoma, Pubmed ID: 18669648

Boster Scientific Support

Answered: 2014-07-24

Question

We ordered your anti-Notch1 Monoclonal antibody for WB on cervix carcinoma in a previous experiment. I am using mouse, and We intend to use the antibody for IF next. I am interested in examining cervix carcinoma as well as brain in our next experiment. Do you have any suggestion on which antibody would work the best for IF?

C. Wu

Verified customer

Asked: 2013-04-08

Answer

I looked at the website and datasheets of our anti-Notch1 Monoclonal antibody and I see that M00033 has been validated on mouse in both WB and IF. Thus M00033 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2013-04-08

Order DetailsPrice
M00033

100μL

$370
M00033-30ul

30μL sample

$99
M00033-carrier-free

Carrier Free

$370

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