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- Table of Contents
WNT3A antibody (PA2120) performs well in WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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Anti-WNT3A antibody, PA2120, Western blotting
All lanes: Anti WNT3A (PA2120) at 0.5ug/ml
WB: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti WNT3A (PA2120) at 0.5ug/ml
WB: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 39KD
Observed bind size: 39KD
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Anti-Protein Wnt-3a Wnt3a Antibody Picoband®
WNT3A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | WB |
Tested Applications
WNT3A antibody was positively detected by WB in:
SMMC Whole Cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Protein Wnt-3a Wnt3a Antibody Picoband®
View all WNT3A Antibodies
View all WNT3A Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Wnt3a, identical to the related mouse sequence.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
39 kDa
Calculated Molecular Weight
39.4 kDa
Background of WNT3A
Wingless-type MMIV integration site family, member 3A is a protein that in humans is encoded by the WNT3A gene. This gene is a member of the WNT gene family. The gene was assigned to human chromosome 1q42.13. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. It encodes a protein showing 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product.
Cite This Product
Anti-Protein Wnt-3a Wnt3a Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2120)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Monkey
100%
High Homology
Dog
94%
High Homology
Rabbit
88%
Moderate Homology
Zebrafish
75%
Moderate Homology
Drosophila
38%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for WNT3A antibody
Publications
Application:
Species:
Jin-Tian-Ge ameliorates ovariectomy-induced bone loss in rats and modulates osteoblastogenesis and osteoclastogenesis in vitro
Published: 2022-10-05
Journal: Chinese Medicine
Application: WB
Impact Factor: 4.55
Species: Mouse
PMID: 36195960
Bradykinin/bradykinin 1 receptor promotes brain microvascular endothelial cell permeability and proinflammatory cytokine release by downregulating Wnt3a
Published: 2022-09-16
Journal: JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
Application: WB
Impact Factor: 3.57
Species: Human,Rat
PMID: 36111657
Nystose attenuates bone loss and promotes BMSCs differentiation to osteoblasts through BMP and Wnt/β-catenin pathway in ovariectomized mice
Published: 2022-09-07
Journal: Food Science and Human Wellness
Application: WB
Impact Factor: 8.02
Species: Rat
MicroRNA-708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88
Published: 2020-05-28
Journal: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Application: WB
Impact Factor: 4.49
Species: Human
PMID: 32463570
TMEM9 mediates IL-6 and IL-1β secretion and is modulated by the Wnt pathway
Published: 2018-08-15
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 3.12
Species: Human
PMID: 30119033
Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells
Published: 2018-03-20
Journal: INTERNATIONAL JOURNAL OF ONCOLOGY
Application: WB
Impact Factor: 3.33
Species: Human
PMID: 29568859
Expression of Toll‑like receptor 4 on mast cells in gingival tissues of human chronic periodontitis
Published: 2018-02-27
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 1.92
Species: Mouse
PMID: 29488617
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In stock
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PA2120
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 22, 2026