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Product Info Summary
| SKU: | PA2120 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Protein Wnt-3a Wnt3a Antibody Picoband®
SKU/Catalog Number
PA2120
BA2628-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Protein Wnt-3a Wnt3a Antibody catalog # PA2120. Tested in WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Protein Wnt-3a Wnt3a Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2120)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Wnt3a, identical to the related mouse sequence.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2120 is reactive to WNT3A in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
39.4 kDa
Background of WNT3A
Wingless-type MMIV integration site family, member 3A is a protein that in humans is encoded by the WNT3A gene. This gene is a member of the WNT gene family. The gene was assigned to human chromosome 1q42.13. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. It encodes a protein showing 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2120 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse
Positive Control
WB: SMMC Whole Cell
Validation Images & Assay Conditions
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Anti-WNT3A antibody, PA2120, Western blotting
All lanes: Anti WNT3A (PA2120) at 0.5ug/ml
WB: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 39KD
Observed bind size: 39KD
Specific Publications For Anti-Protein Wnt-3a Wnt3a Antibody Picoband® (PA2120)
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Customer Q&As
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16 Customer Q&As for Anti-Protein Wnt-3a Wnt3a Antibody Picoband®
Question
Will anti-Wnt3a antibody PA2120 work for WB with placenta?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
According to the expression profile of placenta, WNT3A is highly expressed in placenta. So, it is likely that anti-Wnt3a antibody PA2120 will work for WB with placenta.
Boster Scientific Support
Answered: 2020-04-14
Question
Do you have a BSA free version of anti-Wnt3a antibody PA2120 available?
Verified Customer
Verified customer
Asked: 2020-03-12
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Wnt3a antibody PA2120 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-12
Question
I have a question about product PA2120, anti-Wnt3a antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2120 anti-Wnt3a antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-22
Question
I would like using your anti-Wnt3a antibody for positive regulation of receptor internalization studies. Has this antibody been tested with western blotting on smmc whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
We appreciate your inquiry. This PA2120 anti-Wnt3a antibody is tested on smmc whole cell lysate. It is guaranteed to work for WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-31
Question
We are currently using anti-Wnt3a antibody PA2120 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on primate tissues as well?
D. Jones
Verified customer
Asked: 2019-12-11
Answer
The anti-Wnt3a antibody (PA2120) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-11
Question
My colleagues were satisfied with the WB result of your anti-Wnt3a antibody. However we have been able to see positive staining in placenta secreted using this antibody. Is that expected? Could you tell me where is WNT3A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
From what I have seen in literature, placenta does express WNT3A. Generally WNT3A expresses in secreted, extracellular space, extracellular. Regarding which tissues have WNT3A expression, here are a few articles citing expression in various tissues:
Mammary gland, Pubmed ID: 8168088, 16227623
Boster Scientific Support
Answered: 2019-11-14
Question
Is this PA2120 anti-Wnt3a antibody reactive to the isotypes of WNT3A?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
The immunogen of PA2120 anti-Wnt3a antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Wnt3a(252-267aa WVETLRPRYTYFKVPT), identical to the related mouse sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-10
Question
I see that the anti-Wnt3a antibody PA2120 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-10-08
Question
I am interested in to test anti-Wnt3a antibody PA2120 on mouse placenta for research purposes, then I may be interested in using anti-Wnt3a antibody PA2120 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
The products we sell, including anti-Wnt3a antibody PA2120, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-10
Question
I was wanting to use your anti-Wnt3a antibody for WB for mouse placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse placenta identification?
B. Collins
Verified customer
Asked: 2019-04-02
Answer
It shows on the product datasheet, PA2120 anti-Wnt3a antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-02
Question
Is a blocking peptide available for product anti-Wnt3a antibody (PA2120)?
L. Kulkarni
Verified customer
Asked: 2019-01-03
Answer
We do provide the blocking peptide for product anti-Wnt3a antibody (PA2120). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-01-03
Question
Would PA2120 anti-Wnt3a antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-06-13
Answer
As indicated on the product datasheet, PA2120 anti-Wnt3a antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-06-13
Question
See below the WB image, lot number and protocol we used for placenta using anti-Wnt3a antibody PA2120. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-04-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-18
Question
Will anti-Wnt3a antibody PA2120 work on primate WB with placenta?
D. Johnson
Verified customer
Asked: 2018-01-24
Answer
Our lab technicians have not tested anti-Wnt3a antibody PA2120 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Wnt3a antibody PA2120 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-24
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-Wnt3a antibody PA2120. Let me know if you need anything else.
G. Edwards
Verified customer
Asked: 2013-12-24
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-12-24
Question
We have been able to see staining in human mammary gland. Any tips? Is anti-Wnt3a antibody supposed to stain mammary gland positively?
L. Mangal
Verified customer
Asked: 2013-09-05
Answer
From literature mammary gland does express WNT3A. From Uniprot.org, WNT3A is expressed in placenta, mammary gland, among other tissues. Regarding which tissues have WNT3A expression, here are a few articles citing expression in various tissues:
Mammary gland, Pubmed ID: 8168088, 16227623
Boster Scientific Support
Answered: 2013-09-05
