Anti-NOTCH 2 Antibody

Product Name

Anti-NOTCH 2 Antibody

View all NOTCH2 Antibodies

SKU/Catalog Number

A00518

Size

200uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-NOTCH 2 Antibody (Catalog # A00518). Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-NOTCH 2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00518)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.1% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acid residues 2390-2415 of human Notch 2 (the total protein is 2471 aa).  A residue of cysteine was added to the amino terminal end to facilitate coupling.

Reactive Species

A00518 is reactive to NOTCH2 in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

265.4 kDa

Background of NOTCH2

Anti Notch 2 Antibody recognizes Notch 2 that is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane. Notch functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00518 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:30,000 - 1:90,000
IHC: 1:200-1:800
WB: 1:400 - 1:2,000
This antibody has been tested for use in ELISA and western blot.  For western blot experiments, one can expect a band of ≈110 kDa in size corresponding to active Notch 2 in the appropriate cell lysate. A 1:500 dilution was effective for staining FFPE human kidney tissue by Immunohistochemistry (IHC). Specific conditions for reactivity should be optimized by the end user.

Validation Images & Assay Conditions

See full target information NOTCH2

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