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- Table of Contents
OCLN antibody (A01246) performs well in ELISA, IF, IHC-P, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Immunofluorescence of OCLN in Human Liver tissue with OCLN antibody at 20 μg/mL.
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Anti-Occludin OCLN Antibody
OCLN antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
OCLN antibody can be used for detection of OCLN by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Occludin OCLN Antibody
View all OCLN Antibodies
View all OCLN Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
OCLN Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
OCLN antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human OCLN. The immunogen is located within the last 50 amino acids of OCLN.
Cross-Reactivity
At least three isoforms of OCLN are known to exist.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
59.1 kDa
Background of OCLN
Tight junctions act as a semi-permeable barrier to the transport of ions, solutes, and water and are considered to function as a fence that divides apical and basolateral domains of plasma membranes. Tight junctions coordinate a variety of signaling and trafficking molecules regulating cell differentiation, proliferation, and polarity and contain a number of junctional proteins including Occludin, Claudins, junctional adhesion molecules (JAMs), as well as multiple scaffold proteins. Occludin, the first identified component of tight junction strands, is thought function as a signal transmitter in multiple signaling pathways and can associate with multiple kinases and phosphatases such as PI3-kinase and protein phosphatases 1 and 2A. At least two isoforms of OCLN are known to exist.
Cite This Product
Anti-Occludin OCLN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01246)
Publications
Application:
Species:
Using gut microbiota and non-targeted metabolomics techniques to study the effect of xylitol on alleviating DSS-induced inflammatory bowel disease in mice
Published: 2025-03-10
Journal: BMC IMMUNOLOGY
Application: IHC
Impact Factor: 2.70
Species: Mouse
PMID: 40065221
Pramipexole Protects Against Traumatic Brain Injury-Induced Blood–Brain Barrier (BBB) Dysfunction
Published: 2022-05-07
Journal: NEUROTOXICITY RESEARCH
Application: WB,IF
Impact Factor: 3.98
Species: Mouse
PMID: 35524855
Review
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1 Review For Anti-Occludin OCLN Antibody
In WB using Occludin antibody (Cat# A01246), clear bands showed decreased Occludin in the model group and best recovery in the high-dose herbal treatment group.
| SKU | A01246 |
|---|---|
| Application | Western Blot |
| Sample | rat colon tissue |
| Sample Processing Description | RIPA lysis buffer containing protease inhibitor PMSF (100:1) was used to lyse samples for 10 min, followed by centrifugation at 12,000 rpm for 15 min; the supernatant was mixed with 5× loading buffer, boiled at 100 °C for 10 min, and loaded onto SDS-PAGE. |
| Other Reagents | 5% Non-fat milk |
| Primary Antibody | Occludin OCLN Antibody |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) |
| Secondary Incubation | 1:5000, 1 h in RT |
| Detection | Substrate: ECL substrate, Imaging system:ChemiDoc MP |
| Results Summary | The image shows WB results of Occludin and the loading control Actin in rat colon across different groups; Occludin expression was reduced in the model group, with the high-dose herbal treatment showing the best recovery and clear, well-defined target bands. |
Shiyu Zhang, Liaoning University of Traditional Chinese Medicine
Verified customer
Submitted 2026-02-26
Product Promise
We promise Anti-Occludin OCLN Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A01246
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 18, 2026