Anti-Occludin OCLN Antibody

Occludin antibody

Boster Bio Anti-Occludin OCLN Antibody (Catalog # A01246). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat. Cited in 5 publication(s).

Product Info Summary

SKU: A01246
Size: 0.1 mg
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, IHC-P, WB

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Product Name

Anti-Occludin OCLN Antibody

View all Occludin Antibodies

SKU/Catalog Number

A01246

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Occludin OCLN Antibody (Catalog # A01246). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

OCLN antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Occludin OCLN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01246)

Host

Rabbit

Contents

OCLN Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

SK7

Isotype

IgG

Immunogen

OCLN antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human OCLN. The immunogen is located within the last 50 amino acids of OCLN.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least three isoforms of OCLN are known to exist.

Reactive Species

A01246 is reactive to Ocln in Human, Mouse, Rat

Applications

A01246 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

59kDa

Background of Occludin

Tight junctions act as a semi-permeable barrier to the transport of ions, solutes, and water and are considered to function as a fence that divides apical and basolateral domains of plasma membranes. Tight junctions coordinate a variety of signaling and trafficking molecules regulating cell differentiation, proliferation, and polarity and contain a number of junctional proteins including Occludin, Claudins, junctional adhesion molecules (JAMs), as well as multiple scaffold proteins. Occludin, the first identified component of tight junction strands, is thought function as a signal transmitter in multiple signaling pathways and can associate with multiple kinases and phosphatases such as PI3-kinase and protein phosphatases 1 and 2A. At least two isoforms of OCLN are known to exist.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

OCLN antibody can be used for detection of OCLN by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For Ocln (Source: Uniprot.org, NCBI)

Gene Name

Ocln

Full Name

Occludin

Weight

59kDa

Superfamily

ELL/occludin family

Alternative Names

BLCPMG; Occludin; OCLN Ocln|AI503564, O, Ocl|occludin|occludin

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Ocln, check out the Ocln Infographic

Ocln infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Ocln: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A01246 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Qing-qing Zhang,Ye Yang,Rong-rong Ren,Qing-qing Chen,Jing-jing Wu,Yu-yu Zheng,Xiao-hui Hou,Yu-feng Zhang,Ming-song Xue,Deng-ke Yin,Self-assembled aggregations in Coptis chinensis decoction dynamically regulate intestinal tissue permeability through peyer‘s patch-associated immunity,Chinese Herbal Medicines,2021,,ISSN 1674-6384,https://doi.org/10.1016/j.chmed.2021.06.004.
Species: Rat
A01246 usage in article: APP:WB, SAMPLE:JEJUNAL TISSUE, DILUTION:NA

Xiaozhu Tian,Rong Li,Yiming Jiang,Fei Zhao,Zhengsheng Yu,Yiqing Wang,Zixing Dong,Pu Liu,Xiangkai Li,Bifidobacterium breve ATCC15700 pretreatment prevents alcoholic liver disease through modulating gut microbiota in mice exposed to chronic alcohol intake,J
Species: Mouse
A01246 usage in article: APP:WB, SAMPLE:LIVER TISSUE AND ILEUM, DILUTION:NA

Xin-li Liang,Miao-miao Ji,Lai Chen,Ye Liao,Xiao-qiang Kong,Xi-qiang Xu,Zheng-gen Liao,Danny W. Wilson, Traditional Chinese herbal medicine Astragalus Radix and its effects on intestinal absorption of aconite alkaloids in rats, Chinese Herbal Medicines,202
Species: Rat
A01246 usage in article: APP:WB, SAMPLE:DUODENAL TISSUE, DILUTION:NA

Yang R,Li Y,Mehmood S,Yan C,Huang Y,Cai J,Ji J,Pan W,Zhang W,Chen Y.Polysaccharides from Armillariella tabescens mycelia ameliorate renal damage in type 2 diabetic mice.Int J Biol Macromol.2020 Nov 1;162: 1682-1691.doi:10.1016/j.ijbiomac.2020.08.006.Epub
Species: Mouse
A01246 usage in article: APP:WB, SAMPLE:RENAL AND COLONIC TISSUE, DILUTION:NA

Yang R,Jia Q,Mehmood S,Ma S,Liu X.Genistein ameliorates inflammation and insulin resistance through mediation of gut microbiota composition in type 2 diabetic mice.Eur J Nutr.2020 Oct 17.doi:10.1007/s0 0394-020-02403-0.Epub ahead of print.PMID:33068158.
Species: Mouse
A01246 usage in article: APP:WB, SAMPLE:COLON TISSUE, DILUTION:NA

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Order DetailsPrice
A01246-0.1 mg

0.1 mg

$449

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