Anti-Tight junction protein ZO-2 TJP2 Antibody

Tight Junction Protein 2 antibody

Boster Bio Anti-Tight junction protein ZO-2 TJP2 Antibody catalog # PA1971. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1971
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, ICC, WB

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Product Name

Anti-Tight junction protein ZO-2 TJP2 Antibody

View all Tight Junction Protein 2 Antibodies

SKU/Catalog Number

PA1971

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Tight junction protein ZO-2 TJP2 Antibody catalog # PA1971. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Tight junction protein ZO-2 TJP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1971)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse TJP2, identical to the related rat sequence.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1971 is reactive to TJP2 in Human, Mouse, Rat

Applications

PA1971 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

150 kDa

Calculated molecular weight

133.958kDa

Background of Tight Junction Protein 2

TJP2 (Tight Junction Protein 2), also known as Zona Occludens 2 or ZO2, is a protein that in humans is encoded by the TJP2 gene. Tight junction proteins (TJPs) belong to a family of membrane-associated guanylate kinase (MAGUK) homologs that are involved in the organization of epithelial and endothelial intercellular junctions. Duclos et al. (1994) mapped the TJP2 gene telomeric to the Friedreich ataxia critical region on chromosome 9q13-q21. TJP2 lies about 70 kb centromeric to the X123 gene and is transcribed in the centromere-to-telomere direction. Using in vitro assays and immunoprecipitation studies, Itoh et al. (1999) showed that the mouse Tjp1, Tjp2, and Tjp3 PDZ1 domains interacted with the C-terminal cytoplasmic domains of Cldn1 through Cldn8. In the mouse inner ear, Walsh et al. (2010) found that Tjp2 expression decreased rapidly between E16.5 and age 1 week to a level in adult mice that was approximately 50% of the level at birth (P0).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For TJP2 (Source: Uniprot.org, NCBI)

Gene Name

TJP2

Full Name

Tight junction protein ZO-2

Weight

133.958kDa

Superfamily

MAGUK family

Alternative Names

C9DUPq21.11; DFNA51; DUP9q21.11; MGC26306; tight junction protein 2 (zona occludens 2); Tight junction protein 2; TJP2; X104; X104tight junction protein ZO-2; ZO2; ZO-2; ZO2Friedreich ataxia region gene X104 (tight junction protein ZO-2); Zona occludens protein 2; Zonula occludens protein 2 TJP2 C9DUPq21.11, DFNA51, DUP9q21.11, PFIC4, X104, ZO2 tight junction protein 2 tight junction protein ZO-2|Friedreich ataxia region gene X104 (tight junction protein ZO-2)|zona occludens 2|zonula occludens protein 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on TJP2, check out the TJP2 Infographic

TJP2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for TJP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-Tight junction protein ZO-2 TJP2 Antibody

Question

We are currently using anti-TJP2 antibody PA1971 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on pig tissues as well?

H. Kulkarni

Verified customer

Asked: 2016-10-27

Answer

The anti-TJP2 antibody (PA1971) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-10-27

Order DetailsPrice
PA1971

100μg

$370
PA1971-10ug

10μg sample (liquid)

$99
PA1971-Biotin

100 μg Biotin conjugated

$570
PA1971-Cy3

100 μg Cy3 conjugated

$570
PA1971-Dylight488

100 μg Dylight488 conjugated

$570
PA1971-Dylight550

100 μg Dylight550 conjugated

$570
PA1971-Dylight594

100 μg Dylight594 conjugated

$570
PA1971-FITC

100 μg FITC conjugated

$570
PA1971-HRP

100 μg HRP conjugated

$570
PA1971-APC

100 μg APC conjugated

$670
PA1971-PE

100 μg PE conjugated

$670
PA1971-carrier-free

Carrier Free

$370

More conjugates/formats

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PA1971
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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