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- Table of Contents
Fgf19(Fgf15) antibody (PB10062) performs well in IHC and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
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IHC analysis of Fgf19(Fgf15) using anti-Fgf19(Fgf15) antibody (PB10062).
Fgf19(Fgf15) was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Fgf19(Fgf15) Antibody (PB10062) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Fgf19(Fgf15) was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Fgf19(Fgf15) Antibody (PB10062) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-Fgf19(Fgf15) Antibody
Fgf19(Fgf15) antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | IHC |
Tested Applications
Fgf19(Fgf15) antibody was positively detected by IHC in:
mouse colon tissue, rat colon tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Fgf19(Fgf15) Antibody
View all FGF19(FGF15) Antibodies
View all FGF19(FGF15) Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat Fgf19(Fgf15) recombinant protein (Position: R26-K218). Rat Fgf19(Fgf15) shares 53.2% and 96.4% amino acid (aa) sequence identity with human and mouse FGF19, respectively.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Background of Fgf19(Fgf15)
FGF15/19 refers to two orthologous fibroblast growth factors which share 50% aminoacid identity and have similar functions. FGF15 was described in the mouse; FGF19 was found in humans and other species. They share physiological functions and so are often referred to as FGF15/19 or as FGF15/FGF19. They were first described in developing fetal brain. They are now known to be produced in the ileum, and under certain circumstances in the liver and biliary tree. It is thought their principal function is in response to bile acid absorption occurring after meals. FGF15 and FGF19 have similar roles in regulating bile acid synthesis and also glucose metabolism in the liver.
Cite This Product
Anti-Fgf19(Fgf15) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10062)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Rat
100%
High Homology
Mouse
96%
High Homology
Human
54%
Low homology
Monkey
54%
Low homology
Dog
53%
Low homology
Rabbit
52%
Low homology
Cow
49%
Low homology
Sheep
48%
Low homology
Zebrafish
35%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for Fgf19(Fgf15) antibody
Publications
Application:
Species:
Zhuyu Pill Alleviates Nonalcoholic Fatty Liver Disease by Regulating Bile Acid Metabolism through the Gut–Liver Axis
Published: 2023-08-02
Journal: ACS Omega
Application: WB
Impact Factor: 4.10
Species: Mouse
PMID: 37599938
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Product Promise
We promise Anti-Fgf19(Fgf15) Antibody performs excellently in relevant applications for IHC. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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PB10062
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026