This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
OPTC antibody (A09717-1) performs well in ELISA, WB and shows reactivity with Human, Monkey.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
Western blot analysis of OPTC using anti-OPTC antibody (A09717-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human ARPE-19 whole cell lysates,
Lane 2: monkey RF/6A whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPTC antigen affinity purified polyclonal antibody (Catalog # A09717-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPTC at approximately 37 kDa. The expected band size for OPTC is at 37,45 kDa.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human ARPE-19 whole cell lysates,
Lane 2: monkey RF/6A whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPTC antigen affinity purified polyclonal antibody (Catalog # A09717-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPTC at approximately 37 kDa. The expected band size for OPTC is at 37,45 kDa.
Bundle Save
Anti-OPTC Antibody Picoband®
OPTC antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey | Rabbit | ELISA, WB |
Tested Applications
OPTC antibody was positively detected by WB in:
human ARPE-19 whole cell, monkey RF/6A whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Monkey
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-OPTC Antibody Picoband®
View all OPTC Antibodies
View all OPTC Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human OPTC recombinant protein (Position: E32-Q250).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
37 kDa
Calculated Molecular Weight
37.3 kDa
Background of OPTC
Opticin is a protein that in humans is encoded by the OPTC gene. Opticin belongs to class III of the small leucine-rich repeat protein (SLRP) family. Members of this family are typically associated with the extracellular matrix. Opticin is present in significant quantities in the vitreous of the eye and also localizes to the cornea, iris, ciliary body, optic nerve, choroid, retina, and fetal liver. Opticin may noncovalently bind collagen fibrils and regulate fibril morphology, spacing, and organization. The opticin gene is mapped to a region of chromosome 1 that is associated with the inherited eye diseases age-related macular degeneration (AMD) and posterior column ataxia with retinosa pigmentosa (AXPC1).
Cite This Product
Anti-OPTC Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09717-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
95%
High Homology
Dog
73%
Moderate Homology
Mouse
72%
Moderate Homology
Sheep
71%
Moderate Homology
Cow
70%
Moderate Homology
Rat
68%
Low homology
Rabbit
66%
Low homology
Zebrafish
42%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for OPTC antibody
Review
Have you used Anti-OPTC Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-OPTC Antibody Picoband® performs excellently in relevant applications for ELISA, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A09717-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026