Anti-p53 TP53 Rabbit Monoclonal Antibody

Product Name

Anti-p53 TP53 Rabbit Monoclonal Antibody

View all p53 Antibodies

SKU/Catalog Number

M00001-2

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-p53 TP53 Rabbit Monoclonal Antibody catalog # M00001-2. Tested in WB, ICC/IF applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-p53 TP53 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00001-2)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

DDE-20

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human p53

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00001-2 is reactive to TP53 in Human

Applications

M00001-2 is guaranteed for IF, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

43.653kDa

Background of p53

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser-845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR (Roche et al., 1996; Banke et al., 2000). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory (Lee at al., 2003; Esteban at al., 2003).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

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Western blot analysis of p53 expression in (1) Raji cell lysate; (2) HepG2 cell lysate.

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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

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Immunofluorescent analysis of Hela cells, using p53 Antibody .

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

Gene/Protein Information For TP53 (Source: Uniprot.org, NCBI)

Alternative Names

Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53 TP53 BCC7, BMFS5, LFS1, P53, TRP53 tumor protein p53 cellular tumor antigen p53|antigen NY-CO-13|mutant tumor protein 53|p53 tumor suppressor|phosphoprotein p53|transformation-related protein 53|tumor protein 53|tumor supressor p53

For more info on TP53, check out the TP53 Infographic

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In this infographic, you will see the following information for TP53: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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