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TP53 antibody (PB9008) performs well in IP, IHC, WB and shows reactivity with Human.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 56 Publications

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pb9008 tp53 primary antibodies ihc testing 1

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IHC analysis of TP53 using anti-TP53 antibody (PB9008).
TP53 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-TP53 Antibody (PB9008) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Anti-P53/TP53 Antibody Picoband®

Name: Anti-P53/TP53 Antibody Picoband®
Brand: Boster Bio
SKU: PB9008
Price: 370 USD
Availability: InStock
Rating: 100 (1 reviews)

TP53 antibody

56 Publications|

1 Review

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Reactive Species	Host	Application
Human	Rabbit	IP, IHC, WB

Tested Applications

TP53 antibody was positively detected by WB in:

human 293T whole cell, human T47D whole cell, human PC-9 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

TP53 antibody was positively detected by IHC in:

human colon cancer tissue, human ovarian cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

TP53 antibody was positively detected by IP in:

293T cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Monkey

Predicted

Rabbit

Predicted

Product Information

Product Name

Anti-P53/TP53 Antibody Picoband®
View all TP53 Antibodies

Form

Lyophilized

Uniprot

P04637

Gene ID

7157

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human P53 recombinant protein (Position: A74-D393). Human P53 shares 83% and 85% amino acid (aa) sequences identity with mouse and rat P53, respectively.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

53 kDa

Calculated Molecular Weight

43.7 kDa

Background of TP53

The p53 tumor antigen is found in increased amounts in a wide variety of transformed cells. The protein is also detectable in many actively proliferating, nontransformed cells, but it is undetectable or present at low levels in resting cells. This protein induces cell cycle arrest or apoptosis in response to sublethal or severe DNA damage, respectively, by differential transcription of target genes and through transcription-independent apoptotic functions. The p53 protein contains 393 amino acids. Human p53 tumour antigen is Locatedto band 17p13. p53 mutations are common in pancreatic cancer and are absent in chronic pancreatitis.

Cite This Product

Anti-P53/TP53 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9008)

Protocols

Western Blot protocol for TP53 antibody

Review Protocol

Immunohistochemistry (IHC) protocol for TP53 antibody

Review Protocol

Immunoprecipitation (IP) protocol for TP53 antibody

Review Protocol

Publications

Therapeutic potential of recombinant human collagen XVII in blue light-induced skin photoaging: preserving epidermal-dermal structural integrity and functional homeostasis

Published: 2026-04-28

Journal: Frontiers in Bioengineering and Biotechnology

Application: WB

Impact Factor: 4.80

Species: Human

PMID: 42131503

SKU	PB9008
Application	Immunohistochemistry (Paraffin-embedded)
Blocking step	5% BSA as a blocking agent for 30 min at 37°C
Sample	Human Ovary
Fixative	Fixed with 4% paraformaldehyde
Primary Ab Incubation	4°C overnight
Primary Ab Incubation diluent	5% BSA in TBS
Primary Ab Concentration	1ug/ml
Secondary Antibody	SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution	The kit was ready to use, no dilution needed
Secondary Ab Incubation	4°C overnight

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Anti-P53/TP53 Antibody Picoband®

TP53 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Therapeutic potential of recombinant human collagen XVII in blue light-induced skin photoaging: preserving epidermal-dermal structural integrity and functional homeostasis

A conserved eIF1A+ luminal cell-centered hypoxic and “cold” tumor microenvironment promotes pan-subtype prostate cancer progression

Altered Magnesium Environments Restrict Colorectal HT-29 Spheroid Growth by Disturbing Cellular Mg2+ Homeostasis

Effect of Selenium-enriched Ganoderma lucidum powder in testicular tissues, oxidative stress biomarkers and antiapoptotic capacities in roosters

Dietary Supplementation with Selenium Yeast Mitigates Diquat-Induced Oxidative Damage in Oviductal Magnum of Hens

Structural characterization and effect of activating autophagy and regulating oxidative stress of polysaccharide from fibrous roots of Atractylodes chinensis

TDH-11 inhibits the proliferation and colonization of colorectal cancer by reducing the activity of HDAC

Involvement of the heat shock response (HSR) regulatory pathway in cadmium-elicited cerebral damage

Cardioprotection Attributed to Aerobic Exercise-Mediated Inhibition of ALCAT1 and Oxidative Stress-Induced Apoptosis in MI Rats

Punicalagin Attenuates Neuronal Apoptosis by Upregulating 5-Hydroxymethylcytosine in the Diabetic Mouse Brain

Identification of core genes associated with type 2 diabetes mellitus and gastric cancer by bioinformatics analysis

Ferritinophagy-Mediated ROS Production Contributed to Proliferation Inhibition, Apoptosis, and Ferroptosis Induction in Action of Mechanism of 2-Pyridylhydrazone Dithiocarbamate Acetate

Lipopolysaccharide Affects the Proliferation and Glucose Metabolism of Cervical Cancer Cells Through the FRA1/MDM2/p53 Pathway

Antitumor effects of dioscin in A431 cells via adjusting ATM/p53‑mediated cell apoptosis, DNA damage and migration

Chemopreventive Role of Apigenin against the Synergistic Carcinogenesis of Human Papillomavirus and 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone

Detection of p53 mutation and serum monitoring alert caused by Marek’s disease virus in poultry

MicroRNA-708 prevents ethanol-induced hepatic lipid accumulation and inflammatory reaction via direct targeting ZEB1

p53-Mediated PI3K/AKT/mTOR Pathway Played a Role in PtoxDpt-Induced EMT Inhibition in Liver Cancer Cell Lines

ZWINT is the next potential target for lung cancer therapy

Synergistic anticancer and antibacterial activities of cordycepin and selected natural bioactive compounds

Combined Phycocyanin and Hematoporphyrin Monomethyl Ether for Breast Cancer Treatment via Photosensitizers Modified Fe3O4 Nanoparticles Inhibiting the Proliferation and Migration of MCF-7 Cells

Cisatracurium-induced proliferation impairment and death of colorectal cancer cells, HCT116 is mediated by p53 dependent intrinsic apoptotic pathway in vitro

Antitumor Effects of Laminaria Extract Fucoxanthin on Lung Cancer

Antiproliferative and Apoptosis-inducing Effect of exo-Protoporphyrin IX based Sonodynamic Therapy on Human Oral Squamous Cell Carcinoma

Dioscin suppresses hepatocellular carcinoma tumor growth by inducing apoptosis and regulation of TP53, BAX, BCL2 and cleaved CASP3

A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo

Fra-1 is upregulated in lung cancer tissues and inhibits the apoptosis of lung cancer cells by the P53 signaling pathway

Lactotransferrin expression is downregulated and affects the mitogen‑activated protein kinase pathway in gastric cancer Corrigendum in /10.3892/ol.2021.12504

PBK/TOPK expression correlates with mutant p53 and affects patients' prognosis and cell proliferation and viability in lung adenocarcinoma

Anti-Tumor Effect of Cactus Polysaccharides on Lung Squamous Carcinoma Cells (SK-MES-1)

CD38 is highly expressed and affects the PI3K/Akt signaling pathway in cervical cancer

Isoflavones Extracted from Chickpea Cicer arietinum L. Sprouts Induce Mitochondria-Dependent Apoptosis in Human Breast Cancer Cells

Cisplatin-induced senescence in ovarian cancer cells is mediated by GRP78

Antitumor effects of artesunate on human breast carcinoma MCF-7 cells and IGF-IR expression in nude mice xenografts

Nutlin-3 downregulates p53 phosphorylation on serine392 and induces apoptosis in hepatocellular carcinoma cells

Proteomics Analysis of Normal and Senescent NG108-15 Cells: GRP78 Plays a Negative Role in Cisplatin-Induced Senescence in the NG108-15 Cell Line

Targeting of Histone Deacetylases to Reactivate Tumour Suppressor Genes and Its Therapeutic Potential in a Human Cervical Cancer Xenograft Model

Ran GTPase protein promotes human pancreatic cancer proliferation by deregulating the expression of Survivin and cell cycle proteins

c-Ski activates cancer-associated fibroblasts to regulate breast cancer cell invasion

Sulfite exposure-induced hepatocyte death is not associated with alterations in p53 protein expression

The oncogenic role of NS5A of hepatitis C virus is mediated by up-regulation of survivin gene expression in the hepatocellular cell through p53 and NF-κB pathways

The apoptotic effect and associated signalling of HSP90 inhibitor 17-DMAG in hepatocellular carcinoma cells

Decreased levels of nitric oxide production and nitric oxide synthase-2 expression are associated with the development and metastasis of hepatocellular carcinoma

Pancreatic Ductal Cells Acquire Mesenchymal Characteristics through Cell Fusion with Bone Marrow-Derived Mesenchymal Stem Cells and SIRT1 Attenuates the Apoptosis of Hybrid Cells

Regression of A549 lung cancer tumors by anti-miR-150 vector

Silencing MTA1 by RNAi reverses adhesion, migration and invasiveness of cervical cancer cells (SiHa) via altered expression of p53, and E-cadherin/β-catenin complex

SAFROLE OXIDE INDUCES APOPTOSIS IN A549 HUMAN LUNG CANCER CELLS

Downregulation of survivin and activation of caspase-3 through the PI3K/Akt pathway in ursolic acid-induced HepG2 cell apoptosis

Knockdown of Survivin and Upregulation of p53 Gene Expression by Small Interfering RNA Induces Apoptosis in Human Gastric Carcinoma Cell Line SGC-823

Apoptosis induced by baicalin involving up-regulation of P53 and bax in MCF-7 cells

P53 transcription-independent activity mediates selenite-induced acute promyelocytic leukemia NB4 cell apoptosis

Positional and expressive alteration of prohibitin during the induced differentiation of human hepatocarcinoma SMMC-7721 cells

Fluorescent Nanoparticle-Based Indirect Immunofluorescence Microscopy for Detection of Mycobacterium tuberculosis

ZNF307, a novel zinc finger gene suppresses p53 and p21 pathway

Histological type of oncogenity and expression of cell cycle genes in tumor cells from human mesenchymal stem cells

Integrin beta4 mAb inhibited apoptosis induced by deprivation of growth factors in vascular endothelial cells

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Immunohistochemistry for Anti-P53/TP53 Antibody

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