Anti-P53/TP53 Antibody Picoband™

Boster Bio Anti-P53/TP53 Antibody Picoband™ catalog # PB9008. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human. Cited in 50 publication(s). Independently reviewed in 1 review(s).

Product Info Summary

SKU: PB9008
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, IHC-P, ICC, WB

Product Name

Anti-P53/TP53 Antibody Picoband™

See all p53 products

SKU/Catalog Number

PB9008

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-P53/TP53 Antibody Picoband™ catalog # PB9008. Tested in Flow Cytometry, IF, IHC-P, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-P53/TP53 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9008)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human P53 recombinant protein (Position: A74-D393). Human P53 shares 83% and 85% amino acid (aa) sequences identity with mouse and rat P53, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9008 is reactive to TP53 in Human

Applications

PB9008 is guaranteed for Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For TP53 (Source: Uniprot.Org, NCBI)

Uniprot ID

P04637

Gene ID

7157

Gene Name

TP53

Full Name

Cellular tumor antigen p53

Weight

43.653kDa

Superfamily

p53 family

Alternative Names

Antigen NY-CO-13; BCC7; FLJ92943; LFS1; LFS1TRP53; p53 tumor suppressor; p53; P53cellular tumor antigen p53; Phosphoprotein p53; TP53; transformation-related protein 53; TRP53; tumor protein p53; Tumor suppressor p53 TP53 BCC7, BMFS5, LFS1, P53, TRP53 tumor protein p53 cellular tumor antigen p53|antigen NY-CO-13|mutant tumor protein 53|p53 tumor suppressor|phosphoprotein p53|transformation-related protein 53|tumor protein 53|tumor supressor p53

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TP53, check out the TP53 Infographic

TP53 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TP53: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PB9008 has been cited in 50 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Bacterial cellulose based composites enhanced transdermal drug targeting for breast cancer treatment
Species: Human

Jiang X,Yuan J,Dou Y,Zeng D, Xiao S.Lipopolysaccharide Affects the Proliferation and Glucose Metabolism of Cervical Cancer Cells Through the FRA1/MDM2/p53 Pathway.Int J Med Sci 2021;18(4): 1030-1038.doi:10. 7150/ijms.47360.
Species: Human
PB9008 usage in article: APP:WB, SAMPLE:CASKI CELL AND HELA CELL, DILUTION:NA

The natural compound GL22, isolated from Ganoderma mushrooms, suppresses tumor growth by altering lipid metabolism and triggering cell death

Metronomic chemotherapy remodel cancer-associated fibroblasts to decrease chemoresistance of gastric cancer in nude mice

Acetazolamide Suppresses Multi-Drug Resistance-Related Protein 1 and P-Glycoprotein Expression by Inhibiting Aquaporins Expression in a Mesial Temporal Epilepsy Rat Model

Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis

P-glycoprotein Mediates Postoperative Peritoneal Adhesion Formation by Enhancing Phosphorylation of the Chloride Channel-3

Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats.

Lactotransferrin expression is downregulated and affects the mitogen-activated protein kinase pathway in gastric cancer

Correlation ofp53gene mutation and expression of P53 protein in cholangiocarcinoma

MDM4 Overexpressed in Acute Myeloid Leukemia Patients with Complex Karyotype and Wild-TypeTP53

Suspension culture combined with chemotherapeutic agents for sorting of breast cancer stem cells

The molecular mechanism of G2M cell cycle arrest induced by AFB1 in the jejunum

A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo

TALENs-directed?knockout?of the?full-length?transcription?factor?Nrf1? that?represses?malignantbehaviour?of?human?hepatocellular?carcinoma?(HepG2)?cells

HOXB1 Is a Tumor Suppressor Gene Regulated by miR-3175 in Glioma

Dietary NiCl2 causes G2/M cell cycle arrest in the broiler's kidney

Expression of TP53, BCL-2, and VEGFA Genes in Esophagus Carcinoma and its Biological Significance

Effect of ligustilide on Ang II-induced hypertrophy in cardiomyocytes and the potential mechanisms

c?Ski activates cancer?associated fibroblasts to regulate breast cancer cell invasion

Lv Y, Zheng J, Zhou Q, Jia L, Wang C,, Liu N, Zhao H, Ji H, Li B, Cao W,,. Sci Rep. 2017 Jan 19;7:40967. doi: 10.1038/srep40967. Antiproliferative and Apoptosis-inducing Effect of exo-Protoporphyrin IX based Sonodynamic Therapy on Human Oral Squam...

Feng D, Wu J, Tian Y, Zhou H, Zhou Y, Hu W, Zhao W, Wei H, Ling B, Ma C. Plos One. 2013 Nov 19;8(11):E80657. Doi: 10.1371/Journal.Pone.0080657. Ecollection 2013. Targeting Of Histone Deacetylases To Reactivate Tumour Suppressor Genes And Its Thera...

Li W, Wu D, Wei B, Wang S, Sun H, Li X, Zhang F, Zhang C, Xin Y. Afr J Tradit Complement Altern Med. 2014 Aug 23;11(5):99-104. Ecollection 2014. Anti-Tumor Effect Of Cactus Polysaccharides On Lung Squamous Carcinoma Cells (Sk-Mes-1).

Zhang F, Kong Ds, Zhang Zl, Lei N, Zhu Xj, Zhang Xp, Chen L, Lu Y, Zheng Sz. Apoptosis. 2013 Feb;18(2):135-49. Doi: 10.1007/S10495-012-0791-5. Tetramethylpyrazine Induces G0/G1 Cell Cycle Arrest And Stimulates Mitochondrial-Mediated And Caspase-De...

Guan Yq, Li Z, Yang A, Huang Z, Zheng Z, Zhang L, Li L, Liu Jm. Biomaterials. 2012 Sep;33(26):6162-71. Doi: 10.1016/J.Biomaterials.2012.05.037. Epub 2012 Jun 8. Cell Cycle Arrest And Apoptosis Of Ovcar-3 And Mcf-7 Cells Induced By Co-Immobilized T...

Yuan H, Jiang F, Wang R, Shen M, Chen N. Mol Med Rep. 2012 Feb;5(2):420-6. Doi: 10.3892/Mmr.2011.668. Epub 2011 Nov 7. Lentivirus-Mediated Rna Interference Of E2F-1 Suppresses Tca8113 Cell Proliferation.

Qin D, He X, Wang K, Zhao Xj, Tan W, Chen J. J Biomed Biotechnol. 2007;2007(7):89364. Doi: 10.1155/2007/89364. Fluorescent Nanoparticle-Based Indirect Immunofluorescence Microscopy For Detection Of Mycobacterium Tuberculosis.

Gu T, Li Cx, Feng Y, Wang Q, Li Ch, Li Cf. World J Gastroenterol. 2007 Apr 14;13(14):2113-7. Trans-Arterial Gene Therapy For Hepatocellular Carcinoma In A Rabbit Model.

Liu Xm, Yang Ff, Yuan Yf, Zhai R, Huo Lj. Plos One. 2013 May 16;8(5):E63680. Doi: 10.1371/Journal.Pone.0063680. Print 2013. Sumoylation Of Mouse P53B By Sumo-1 Promotes Its Pro-Apoptotic Function In Ovarian Granulosa Cells.

Bai J, Lei P, Zhang J, Zhao C, Liang R. Toxicology. 2013 Oct 4;312:142-8. Doi: 10.1016/J.Tox.2013.08.004. Epub 2013 Aug 21. Sulfite Exposure-Induced Hepatocyte Death Is Not Associated With Alterations In P53 Protein Expression.

Li W, Wang W, Li Y, Wang W, Wang T, Li L, Han Z, Wang S, Ma D, Wang H. Plos One. 2014 Mar 12;9(3):E90114. Doi: 10.1371/Journal.Pone.0090114. Ecollection 2014. Proteomics Analysis Of Normal And Senescent Ng108-15 Cells: Grp78 Plays A Negative Role ...

Li W, Wang W, Dong H, Li Y, Li L, Han L, Han Z, Wang S, Ma D, Wang H. Oncol Rep. 2014 Jun;31(6):2525-34. Doi: 10.3892/Or.2014.3147. Epub 2014 Apr 23. Cisplatin-Induced Senescence In Ovarian Cancer Cells Is Mediated By Grp78.

Zhou L, Yang Y, Tian D, Wang Y. Oncol Rep. 2013 Mar;29(3):875-84. Doi: 10.3892/Or.2013.2227. Epub 2013 Jan 4. Oxidative Stress-Induced 1, N6-Ethenodeoxyadenosine Adduct Formation Contributes To Hepatocarcinogenesis.

Li Yj, Zhang Yx, Wang Py, Chi Yl, Zhang C, Ma Y, Lv Cj, Xie Sy. Oncol Rep. 2012 Jan;27(1):129-34. Doi: 10.3892/Or.2011.1466. Epub 2011 Sep 20. Regression Of A549 Lung Cancer Tumors By Anti-Mir-150 Vector.

Liang S, Peng X, Li X, Yang P, Xie L, Li Y, Du C, Zhang G. Oncotarget. 2015 Jan 20;6(2):1020-30. Silencing Of Cxcr4 Sensitizes Triple-Negative Breast Cancer Cells To Cisplatin.

Hao Y, Huang W, Liao M, Zhu Y, Liu H, Hao C, Liu G, Zhang G, Feng H, Ning X, Li H, Li Z. Fitoterapia. 2013 Apr;86:84-91. Doi: 10.1016/J.Fitote.2013.02.005. Epub 2013 Feb 18. The Inhibition Of Resveratrol To Human Skin Squamous Cell Carcinoma A431 ...

Liao S, Xiao S, Zhu G, Zheng D, He J, Pei Z, Li G, Zhou Y. Oncol Rep. 2014 Dec;32(6):2703-9. Doi: 10.3892/Or.2014.3537. Epub 2014 Oct 10. Cd38 Is Highly Expressed And Affects The Pi3K/Akt Signaling Pathway In Cervical Cancer.

Xiao S, Zhou Y, Yi W, Luo G, Jiang B, Tian Q, Li Y, Xue M. Int J Oncol. 2015 Apr;46(4):1677-84. Doi: 10.3892/Ijo.2015.2873. Epub 2015 Feb 4. Fra-1 Is Downregulated In Cervical Cancer Tissues And Promotes Cervical Cancer Cell Apoptosis By P53 Signa...

Zhang J, Chen X, Shi G, Xie X, Liu H, Zhang X, Lai Y, Zuo Y, Chen Z, Liu S, Wang H. J Ovarian Res. 2013 Feb 6;6(1):9. Doi: 10.1186/1757-2215-6-9. Establishment Of A New Representative Model Of Human Ovarian Cancer In Mice.

Jiang R, Xu W, Zhu W, Chen M, Qian H, Qiao C, Yang H, Wang X, Chen Y. Oncol Rep. 2006 Nov;16(5):1021-8. Histological Type Of Oncogenity And Expression Of Cell Cycle Genes In Tumor Cells From Human Mesenchymal Stem Cells.

Li Bh, Xu Sb, Li F, Zou Xg, Saimaiti A, Simayi D, Wang Yh, Zhang Y, Yuan J, Zhang Wj. Cell Signal. 2012 Mar;24(3):718-25. Doi: 10.1016/J.Cellsig.2011.11.005. Epub 2011 Nov 13. Stat6 Activity-Related Th2 Cytokine Profile And Tumor Growth Advantage ...

Zhang S, Zhang C, Li Y, Wang P, Yue Z, Xie S. Biomed Pharmacother. 2011 Sep;65(6):436-42. Doi: 10.1016/J.Biopha.2011.04.010. Epub 2011 Jun 12. Mir-98 Regulates Cisplatin-Induced A549 Cell Death By Inhibiting Tp53 Pathway.

Xie Sy, Li Yj, Wang Py, Jiao F, Zhang S, Zhang Wj. Oncol Rep. 2010 Jun;23(6):1693-700. Mirna-Regulated Expression Of Oncogenes And Tumor Suppressor Genes In The Cisplatin-Inhibited Growth Of K562 Cells.

Zhang D, Fu M, Song C, Wang C, Lin X, Liu Y. Neural Regen Res. 2012 Oct 25;7(30):2347-53. Doi: 10.3969/J.Issn.1673-5374.2012.30.004. Expressions Of Apoptosis-Related Proteins In Rats With Focal Cerebral Ischemia After Angong Niuhuang Sticker Point...

Yang S, Wang H, Guo Y, Chen S, Zhang My, Shen J, Yu H, Miao J, Wang Hy, Wei W. Int J Biol Sci. 2013 Jul 5;9(6):637-48. Doi: 10.7150/Ijbs.6439. Print 2013. Rmp Plays Distinct Roles In The Proliferation Of Hepatocellular Carcinoma Cells And Normal H...

Dong Hy, Wang Zf. Chin J Cancer Res. 2014 Apr;26(2):200-7. Doi: 10.3978/J.Issn.1000-9604.2014.04.07. Antitumor Effects Of Artesunate On Human Breast Carcinoma Mcf-7 Cells And Igf-Ir Expression In Nude Mice Xenografts.

Wang Q, Xu Y, Zhou W, Zhong L, Wen Z, Yu H, Chen S, Shen J, Chen H, She Q, Jiang J, Miao J, Wei W. Int J Biol Sci. 2014 Nov 18;10(10):1181-92. Doi: 10.7150/Ijbs.10275. Ecollection 2014. The Viral Oncoprotein Hbx Of Hepatitis B Virus Promotes The G...

Guo H, Nan K, Hu T, Meng J, Hui W, Zhang X, Qin H, Sui C. Hepatol Res. 2010 Nov;40(11):1107-16. Doi: 10.1111/J.1872-034X.2010.00721.X. Epub 2010 Sep 28. Prognostic Significance Of Co-Expression Of Nm23 And P57 Protein In Hepatocellular Carcinoma.

Li B, Chu X, Gao M, Li W. Acta Biochim Biophys Sin (Shanghai). 2010 Jan;42(1):80-9. Apoptotic Mechanism Of Mcf-7 Breast Cells In Vivo And In Vitro Induced By Photodynamic Therapy With C-Phycocyanin.

Jiang H, Li J, Zhou T, Wang C, Zhang H, Wang H. Int J Mol Med. 2014 May;33(5):1298-304. Doi: 10.3892/Ijmm.2014.1684. Epub 2014 Mar 6. Colistin-Induced Apoptosis In Pc12 Cells: Involvement Of The Mitochondrial Apoptotic And Death Receptor Pathways.

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1 Reviews For Anti-P53/TP53 Antibody Picoband™

0

Immunohistochemistry for Anti-P53/TP53 Antibody

Excellent

Application Immunohistochemistry (Paraffin-embedded)
Blocking step 5% BSA as a blocking agent for 30 min at 37°C
Sample Human Ovary
Fixative Fixed with 4% paraformaldehyde
Primary Ab Incubation 4°C overnight
Primary Ab Incubation diluent 5% BSA in TBS
Primary Ab Concentration 1ug/ml
Secondary Antibody SABC kit from Boster Bio, (SA1022)
Secondary Ab Dilution The kit was ready to use, no dilution needed
Secondary Ab Incubation 4°C overnight

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6 Customer Q&As for Anti-P53/TP53 Antibody Picoband™

Question

We ordered your anti-P53/TP53 antibody for WB on kidney a few years ago. I am using human, and I plan to use the antibody for IHC next. I would like examining kidney as well as epithelium of mammary gland in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2020-04-20

Answer

I looked at the website and datasheets of our anti-P53/TP53 antibody and it seems that PB9008 has been validated on human in both WB and IHC. Thus PB9008 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-04-20

Question

We are currently using anti-P53/TP53 antibody PB9008 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-01

Answer

The anti-P53/TP53 antibody (PB9008) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-01

Question

We have observed staining in human ovarian adenocarcinoma. Do you have any suggestions? Is anti-P53/TP53 antibody supposed to stain ovarian adenocarcinoma positively?

Verified Customer

Verified customer

Asked: 2018-04-05

Answer

According to literature ovarian adenocarcinoma does express TP53. According to Uniprot.org, TP53 is expressed in epithelium of mammary gland, colon, kidney, lung carcinoma, blood, ovarian adenocarcinoma, glial cell glial tumor, embryonic kidney, among other tissues. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794

Boster Scientific Support

Answered: 2018-04-05

Question

I am interested in using your anti-P53/TP53 antibody for runx3 regulates cdkn1a transcription studies. Has this antibody been tested with western blotting on lung cancer tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2018-03-23

Answer

Thank you for your inquiry. This PB9008 anti-P53/TP53 antibody is validated on lung cancer tissue, hepg2 whole cell lysate, colo320 whole cell lysate. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2018-03-23

Question

We are currently using anti-P53/TP53 antibody PB9008 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?

L. Wu

Verified customer

Asked: 2016-12-05

Answer

The anti-P53/TP53 antibody (PB9008) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-12-05

Question

We were happy with the WB result of your anti-P53/TP53 antibody. However we have observed positive staining in kidney cytoplasm using this antibody. Is that expected? Could you tell me where is TP53 supposed to be expressed?

N. Anderson

Verified customer

Asked: 2015-02-06

Answer

Based on literature, kidney does express TP53. Generally TP53 expresses in cytoplasm. Regarding which tissues have TP53 expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 16131611
Embryonic kidney, Pubmed ID: 17525332
Glial cell, and Glial tumor, Pubmed ID: 19011621
Kidney, Pubmed ID: 15489334
Lung carcinoma, Pubmed ID: 14660794

Boster Scientific Support

Answered: 2015-02-06

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