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Product Info Summary
| SKU: | PB10070 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-HLTF Antibody Picoband®
SKU/Catalog Number
PB10070
PB1124 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HLTF Antibody Picoband® catalog # PB10070. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HLTF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10070)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human HLTF recombinant protein (Position: S911-L1009). Human HLTF shares 92.9% amino acid (aa) sequence identity with mouse HLTF.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10070 is reactive to HLTF in Human, Mouse, Rat
Observed Molecular Weight
114 kDa
Calculated molecular weight
113.9 kDa
Background of HLTF
Helicase-like transcription factor is an enzyme that in humans is encoded by the HLTF gene. This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10070 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human Hela whole cell, human PANC-1 whole cell, human A549 whole cell, rat heart tissue, rat kidney tissue, mouse heart tissue, mouse kidney tissue
ICC/IF: A549 cell
FCM: CACO-2 cell
Validation Images & Assay Conditions
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Western blot analysis of HLTF using anti-HLTF antibody (PB10070).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human PANC-1 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: rat heart tissue lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLTF antigen affinity purified polyclonal antibody (Catalog # PB10070) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLTF at approximately 114 kDa. The expected band size for HLTF is at 114 kDa.
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IF analysis of HLTF using anti-HLTF antibody (PB10070) and anti-Tubulin Alpha antibody (M03989-3).
HLTF was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-HLTF Antibody (PB10070) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Flow Cytometry analysis of CACO-2 cells using anti-HLTF antibody (PB10070).
Overlay histogram showing CACO-2 cells stained with PB10070 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HLTF Antibody (PB10070, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Specific Publications For Anti-HLTF Antibody Picoband® (PB10070)
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6 Customer Q&As for Anti-HLTF Antibody Picoband®
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for female gonad using anti-HLTF antibody PB10070. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-08
Question
I am looking for to test anti-HLTF antibody PB10070 on human female gonad for research purposes, then I may be interested in using anti-HLTF antibody PB10070 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
The products we sell, including anti-HLTF antibody PB10070, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-18
Question
Will anti-HLTF antibody PB10070 work on canine WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
Our lab technicians have not validated anti-HLTF antibody PB10070 on canine. You can run a BLAST between canine and the immunogen sequence of anti-HLTF antibody PB10070 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-16
Question
Is a blocking peptide available for product anti-HLTF antibody (PB10070)?
Verified Customer
Verified customer
Asked: 2018-05-08
Answer
We do provide the blocking peptide for product anti-HLTF antibody (PB10070). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-05-08
Question
I was wanting to use your anti-HLTF antibody for WB for human female gonad on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human female gonad identification?
Verified Customer
Verified customer
Asked: 2017-10-04
Answer
It shows on the product datasheet, PB10070 anti-HLTF antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human female gonad in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-04
Question
We are currently using anti-HLTF antibody PB10070 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Z. Gonzalez
Verified customer
Asked: 2013-03-12
Answer
The anti-HLTF antibody (PB10070) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-03-12
