Click image to see more details
Product Info Summary
| SKU: | M00454 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-PAK1 Monoclonal Antibody
SKU/Catalog Number
M00454
BM5429 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PAK1 Monoclonal Antibody catalog # M00454. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PAK1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00454)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADFA-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PAK1 The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB.
Reactive Species
M00454 is reactive to PAK1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
60.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00454 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:40
FC 1:30
Positive Control
WB: human MCF-7 whole cell, human Hela whole cell, rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of PAK1 using anti-PAK1 antibody (M00454).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAK1 antigen affinity purified monoclonal antibody (M00454) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PAK1 at approximately 68 kDa. The expected band size for PAK1 is at 61 kDa.
Specific Publications For Anti-PAK1 Monoclonal Antibody (M00454)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-PAK1 Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-PAK1 Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
15 Customer Q&As for Anti-PAK1 Monoclonal Antibody
Question
Would M00454 anti-PAK1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
You can see on the product datasheet, M00454 anti-PAK1 Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-06
Question
Is this M00454 anti-PAK1 Monoclonal antibody reactive to the isotypes of PAK1?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
The immunogen of M00454 anti-PAK1 Monoclonal antibody is A synthesized peptide derived from human PAK1 The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-18
Question
I have a question about product M00454, anti-PAK1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00454 anti-PAK1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-24
Question
We purchased anti-PAK1 Monoclonal antibody for WB on liver in the past. I am using human, and I plan to use the antibody for IF next. I am looking for examining liver as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
I have checked the website and datasheets of our anti-PAK1 Monoclonal antibody and I see that M00454 has been validated on human in both WB and IF. Thus M00454 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-21
Question
Does anti-PAK1 Monoclonal antibody M00454 work for IP with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
According to the expression profile of leukemic t-cell, PAK1 is highly expressed in leukemic t-cell. So, it is likely that anti-PAK1 Monoclonal antibody M00454 will work for IP with leukemic t-cell.
Boster Scientific Support
Answered: 2019-07-23
Question
I was wanting to use your anti-PAK1 Monoclonal antibody for IP for human leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukemic t-cell identification?
T. Evans
Verified customer
Asked: 2019-06-06
Answer
As indicated on the product datasheet, M00454 anti-PAK1 Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-06
Question
My team were satisfied with the WB result of your anti-PAK1 Monoclonal antibody. However we have been able to see positive staining in liver cytoplasm using this antibody. Is that expected? Could you tell me where is PAK1 supposed to be expressed?
J. Gonzalez
Verified customer
Asked: 2019-05-17
Answer
From what I have seen in literature, liver does express PAK1. Generally PAK1 expresses in cytoplasm. Regarding which tissues have PAK1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 23503467
Cervix carcinoma, Pubmed ID: 16964243, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 8805275
Boster Scientific Support
Answered: 2019-05-17
Question
We have observed staining in mouse leukemic t-cell. Any tips? Is anti-PAK1 Monoclonal antibody supposed to stain leukemic t-cell positively?
Verified Customer
Verified customer
Asked: 2019-03-15
Answer
From what I have seen in literature leukemic t-cell does express PAK1. From what I have seen in Uniprot.org, PAK1 is expressed in middle temporal gyrus, placenta, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, brain, among other tissues. Regarding which tissues have PAK1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 23503467
Cervix carcinoma, Pubmed ID: 16964243, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 8805275
Boster Scientific Support
Answered: 2019-03-15
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-PAK1 Monoclonal antibody M00454. Let me know if you need anything else.
O. Taylor
Verified customer
Asked: 2018-07-04
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-04
Question
I see that the anti-PAK1 Monoclonal antibody M00454 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-01-16
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-01-16
Question
Is a blocking peptide available for product anti-PAK1 Monoclonal antibody (M00454)?
Verified Customer
Verified customer
Asked: 2017-11-21
Answer
We do provide the blocking peptide for product anti-PAK1 Monoclonal antibody (M00454). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-11-21
Question
Is there a BSA free version of anti-PAK1 Monoclonal antibody M00454 available?
Verified Customer
Verified customer
Asked: 2017-07-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PAK1 Monoclonal antibody M00454 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-10
Question
I was wanting to use to test anti-PAK1 Monoclonal antibody M00454 on human leukemic t-cell for research purposes, then I may be interested in using anti-PAK1 Monoclonal antibody M00454 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-05-22
Answer
The products we sell, including anti-PAK1 Monoclonal antibody M00454, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-05-22
Question
See attached the WB image, lot number and protocol we used for leukemic t-cell using anti-PAK1 Monoclonal antibody M00454. Please let me know if you require anything else.
P. Parker
Verified customer
Asked: 2017-04-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-04-10
Question
We are currently using anti-PAK1 Monoclonal antibody M00454 for rat tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
J. Jha
Verified customer
Asked: 2013-01-22
Answer
The anti-PAK1 Monoclonal antibody (M00454) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-01-22
