Anti-EEA1 Antibody Picoband™

EEA1 antibody

Boster Bio Anti-EEA1 Antibody Picoband™ catalog # A02296-3. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 1 publication(s).

Product Info Summary

SKU: A02296-3
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, ICC, WB

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Product Name

Anti-EEA1 Antibody Picoband™

View all EEA1 Antibodies

SKU/Catalog Number

A02296-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-EEA1 Antibody Picoband™ catalog # A02296-3. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EEA1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02296-3)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human EEA1, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02296-3 is reactive to EEA1 in Human, Mouse, Rat

Applications

A02296-3 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

170 kDa

Calculated molecular weight

162.466kDa

Background of EEA1

The gene EEA1 encodes for the 1400 amino acid protein, Early Endosome Antigen 1. It localizes exclusively to early endosomes and has an important role in endosomal trafficking. EEA1 binds directly to the phospholipid phosphatidylinositol 3-phosphate through its C-terminal FYVE domain and forms ahomodimer through a coiled coil. Furthermore, EEA1 acts as a tethering molecule that couples vesicle docking with SNAREs such as N-ethylmaleimide sensitive fusion protein, bringing the endosomes physically closer and ultimately resulting in the fusion and delivery of endosomal cargo.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For EEA1 (Source: Uniprot.org, NCBI)

Gene Name

EEA1

Full Name

Early endosome antigen 1

Weight

162.466kDa

Alternative Names

early endosome antigen 1; early endosome antigen 1, 162kD; early endosome-associated protein; EEA1; Endosome-associated protein p162; MST105; MSTP105; ZFYVE2; ZFYVE2MST105; Zinc finger FYVE domain-containing protein 2 EEA1 MST105, MSTP105, ZFYVE2 early endosome antigen 1 early endosome antigen 1|early endosome antigen 1, 162kD|early endosome-associated protein|endosome-associated protein p162|zinc finger FYVE domain-containing protein 2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on EEA1, check out the EEA1 Infographic

EEA1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EEA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A02296-3 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wang,Y.,Pang,J.,Wang,Q.,Yan,L.,Wang,L.,Xing,Z.,Wang,C.,Zhang,J., Dong,L.,Delivering Antisense Oligonucleotides across the Blood‐Brain Barrier by Tumor Cell‐Derived Small Apoptotic Bodies.Adv.Sci.2021,2004929.https://doi.org/10.1002/advs.202004929
Species: Mouse
A02296-3 usage in article: APP:TRANSCYTOSIS ANALYSIS, SAMPLE: B16F10 CELL, DILUTION:NA

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5 Customer Q&As for Anti-EEA1 Antibody Picoband™

Question

We are currently using anti-EEA1 antibody A02296-3 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

The anti-EEA1 antibody (A02296-3) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-09

Question

We ordered your anti-EEA1 antibody for Flow Cytometry on cervix carcinoma erythroleukemia in a previous experiment. I am using human, and We intend to use the antibody for WB next. My lab would like examining cervix carcinoma erythroleukemia as well as biceps brachii in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-10-04

Answer

I have checked the website and datasheets of our anti-EEA1 antibody and it appears that A02296-3 has been tested on human in both Flow Cytometry and WB. Thus A02296-3 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-10-04

Question

We have seen staining in rat liver. What should we do? Is anti-EEA1 antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2019-07-26

Answer

From what I have seen in literature liver does express EEA1. From what I have seen in Uniprot.org, EEA1 is expressed in biceps brachii, cervix carcinoma, fetal brain cortex, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have EEA1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 7768953, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal brain cortex, Pubmed ID: 9697774
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-07-26

Question

My boss were content with the WB result of your anti-EEA1 antibody. However we have been able to see positive staining in fetal brain cortex cytoplasm. early endosome membrane using this antibody. Is that expected? Could you tell me where is EEA1 supposed to be expressed?

A. Evans

Verified customer

Asked: 2019-07-23

Answer

From literature, fetal brain cortex does express EEA1. Generally EEA1 expresses in cytoplasm. early endosome membrane. Regarding which tissues have EEA1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 7768953, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Fetal brain cortex, Pubmed ID: 9697774
Liver, Pubmed ID: 24275569

Boster Scientific Support

Answered: 2019-07-23

Question

you antibody using your anti-EEA1 antibody for early endosome to late endosome transport studies. Has this antibody been tested with western blotting on nih3t3 cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-04-23

Answer

Thank you for your inquiry. This A02296-3 anti-EEA1 antibody is validated on rat thymus tissue, spleen tissue, tissue lysate, brain tissue, lung tissue, mouse spleen tissue, nih3t3 cell lysate, human placenta tissue, hela cell lysate, a549 cell lysate. It is guaranteed to work for Flow Cytometry, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-04-23

Order DetailsPrice
A02296-3

100μg

$370
A02296-3-10ug

10μg sample (liquid)

$99
A02296-3-Biotin

100 μg Biotin conjugated

$570
A02296-3-Cy3

100 μg Cy3 conjugated

$570
A02296-3-Dylight488

100 μg Dylight488 conjugated

$570
A02296-3-Dylight550

100 μg Dylight550 conjugated

$570
A02296-3-Dylight594

100 μg Dylight594 conjugated

$570
A02296-3-FITC

100 μg FITC conjugated

$570
A02296-3-HRP

100 μg HRP conjugated

$570
A02296-3-APC

100 μg APC conjugated

$670
A02296-3-PE

100 μg PE conjugated

$670
A02296-3-carrier-free

Carrier Free

$370

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A02296-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
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