Anti-Paxillin Antibody (Monoclonal, PXC-10)

Mouse IgG monoclonal antibody for Paxillin, paxillin (PXN) detection. Tested with WB, ICC in Human;mouse;rat;chicken. No cross reactivity with other proteins.

SKU MA1080
Size 100μg/vial
Reactivity Chicken, Human, Mouse, Rat
Clonality Monoclonal
Clone Number PXC-10
Host Mouse
Ig Isotype Mouse IgG1
Applications ICC, WB

Overview

Product Name Anti-Paxillin Antibody (Monoclonal, PXC-10)
See all Pxn primary antibodies, ELISA kits and proteins
SKU/Catalog Number MA1080
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Monoclonal clone# G2 antibody for Paxillin/PXN detection. Host: Mouse.Size: 100μg/vial. Tested applications: ICC. Reactive species: Chicken. Paxillin/PXN information: Molecular Weight: 64019 MW; Subcellular Localization: Cytoplasm, cytoskeleton . Cell junction, focal adhesion . Cytoplasm, cell cortex . Colocalizes with integrins at the cell periphery. Colocalizes with PXN to membrane ruffles and the leading edge of migrating cells (By similarity).
Cite This Product Anti-Paxillin Antibody (Monoclonal, PXC-10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1080)
Specificity Anti-Paxillin Antibody (Monoclonal, PXC-10) (MA1080) reacts with Chicken, Human, Mouse, Rat Pxn, in native form and recombinant. Superfamily members of Pxn are not reactive to MA1080.
Host Mouse
Contents/Buffer Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Form Lyophilized
Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunogen C-terminal part of recombinant chicken paxillin(amino acids 305-559).
Reactivity Chicken, Human, Mouse, Rat

Assay Details

Our Boster Quality Guarantee for Anti-Paxillin Antibody (Monoclonal, PXC-10) covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Immunocytochemistry , 1μg/ml, Human, mouse, rat, chicken, -
Western blot, 1-2μg/ml, Human, mouse, rat, chicken

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-Paxillin Antibody (Monoclonal, PXC-10) (MA1080).

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.

Images And Assay Conditions

/antibody/ma1080-1-WB-anti-paxillin-antibody-monoclonal-pxc-10.jpg

Anti-Paxillin antibody (monoclonal), MA1080, Western blotting
Lane 1: 293T Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: MM231 Cell Lysate
Lane 5: JURKAT Cell Lysate

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q66H76
Gene Name Pxn
Protein Name Paxillin
Alternative Names Paxillin;Pxn;
Subcellular Localization Cytoplasm, cytoskeleton . Cell junction, focal adhesion . Cytoplasm, cell cortex . Colocalizes with integrins at the cell periphery. Colocalizes with PXN to membrane ruffles and the leading edge of migrating cells (By similarity). .
Molecular Weight 64019 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). .
Research Areas Chicken, Human, Mouse, Rat

*You can search these to find other products in these research areas.
Background The paxillin gene can be alternatively spliced to include 1 of 2 alternative exons, generating beta and gamma isoforms. Paxillin is a 68-kDa focal adhesion protein that is phosphorylated on tyrosine residues in fibroblasts in response to transformation by v-src, treatment with platelet-derived growth factor, or cross-linking of integrins. The 68-kD protein(paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney(MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells.

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In stock
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MA1080
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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Publications

Zhong S, Luo R, Wang X, Tang L, Wu J, Wang J, Huang R, Sun H, Huang N. Colloids Surf B Biointerfaces. 2014 Apr 1;116:553-60. Doi: 10.1016/J.Colsurfb.2014.01.030. Epub 2014 Jan 30. Effects Of Polydopamine Functionalized Titanium Dioxide Nanotubes O...
Liu Y, Zhu Z, Xiong Z, Zheng J, Hu Z, Qiu J. Int J Clin Exp Pathol. 2014 Aug 15;7(9):5750-61. Ecollection 2014. Knockdown Of Protein Tyrosine Phosphatase Receptor U Inhibits Growth And Motility Of Gastric Cancer Cells.
Zhu Z, Liu Y, Li K, Liu J, Wang H, Sun B, Xiong Z, Jiang H, Zheng J, Hu Z. Carcinogenesis. 2014 Aug;35(8):1901-10. Doi: 10.1093/Carcin/Bgu123. Epub 2014 May 29. Protein Tyrosine Phosphatase Receptor U (Ptpru) Is Required For Glioma Growth And Moti...

Customer Q&As

  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
  • Q: Can you provide monoclonal antibodies carrier free?
    A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to [email protected]
  • Q: What are some alternative names that could be used to describe this product?
    A: Some common names include but are not limited to paxillin antibody, pax1 antibody
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