|Validated Species:||Chicken, Human, Mouse, Rat|
Data & Images
|Product Name||Anti-Paxillin Antibody (Monoclonal, PXC-10)|
|Description||Mouse IgG monoclonal antibody for Paxillin, paxillin (PXN) detection. Tested with WB, ICC in Human;mouse;rat;chicken. No cross reactivity with other proteins.|
|Cite This Product||Anti-Paxillin Antibody (Monoclonal, PXC-10) PXC-10 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1080)|
|Replacement Item||This antibody may replace the following items: sc-135171|sc-135170|sc-47366|sc-47364|sc-56228|sc-47368|sc-374639 from Santa Cruz Biotechnology.|
|Validated Species||Chicken, Human, Mouse, Rat|
|Predicted Species||Canine, Horse, Rabbit|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||C-terminal part of recombinant chicken paxillin(amino acids 305-559).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||64019 MW|
|Protein Function||Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). .|
|Sequence Similarities||Belongs to the paxillin family.|
|Subcellular Localization||Cytoplasm, cytoskeleton . Cell junction, focal adhesion . Cytoplasm, cell cortex . Colocalizes with integrins at the cell periphery. Colocalizes with PXN to membrane ruffles and the leading edge of migrating cells (By similarity). .|
|Research Areas|||signal transduction|cytoskeleton / ecm|extracellular matrix|structures|focal adhesions| signal transduction|adapters|cytoplasmic| cancer||
Background for Paxillin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Paxillin Antibody (Monoclonal, PXC-10) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 1μg/ml, Human, mouse, rat, chicken, -|
Western blot, 1-2μg/ml, Human, mouse, rat, chicken
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Paxillin Antibody (Monoclonal, PXC-10) Images
Click the images to enlarge.
Lane 1: 293T Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: MM231 Cell Lysate
Lane 5: JURKAT Cell Lysate