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Product Info Summary
|Reactive Species:||Chicken, Human, Mouse, Rat|
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Anti-Paxillin Pxn Antibody (Monoclonal, PXC-10)
Boster Bio Anti-Paxillin Pxn Antibody (Monoclonal, PXC-10) catalog # MA1080. Tested in ICC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Paxillin Pxn Antibody (Monoclonal, PXC-10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1080)
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
C-terminal part of recombinant chicken paxillin (amino acids 305-559).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
MA1080 is reactive to Pxn in Chicken, Human, Mouse, Rat
MA1080 is guaranteed for ICC, WB Boster Guarantee
Background of Paxillin
The paxillin gene can be alternatively spliced to include 1 of 2 alternative exons, generating beta and gamma isoforms. Paxillin is a 68-kDa focal adhesion protein that is phosphorylated on tyrosine residues in fibroblasts in response to transformation by v-src, treatment with platelet-derived growth factor, or cross-linking of integrins. The 68-kD protein (paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney (MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunocytochemistry , 1μg/ml, Human, mouse, rat, chicken, -
Western blot, 1-2μg/ml, Human, mouse, rat, chicken
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Paxillin using anti-Paxillin antibody (MA1080).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human CACO-2 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Paxillin antigen affinity purified monoclonal antibody (Catalog # MA1080) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Paxillin at approximately 65 kDa. The expected band size for Paxillin is at 65 kDa.
Gene/Protein Information For Pxn (Source: Uniprot.Org, NCBI)
FLJ16691; Paxillin; PXN Pxn|paxillin|paxillin|inverted formin-2-like|myocardial ischemic preconditioning associated protein 7*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Pxn, check out the Pxn Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Pxn: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
MA1080 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhong S, Luo R, Wang X, Tang L, Wu J, Wang J, Huang R, Sun H, Huang N. Colloids Surf B Biointerfaces. 2014 Apr 1;116:553-60. Doi: 10.1016/J.Colsurfb.2014.01.030. Epub 2014 Jan 30. Effects Of Polydopamine Functionalized Titanium Dioxide Nanotubes O...
Liu Y, Zhu Z, Xiong Z, Zheng J, Hu Z, Qiu J. Int J Clin Exp Pathol. 2014 Aug 15;7(9):5750-61. Ecollection 2014. Knockdown Of Protein Tyrosine Phosphatase Receptor U Inhibits Growth And Motility Of Gastric Cancer Cells.
Zhu Z, Liu Y, Li K, Liu J, Wang H, Sun B, Xiong Z, Jiang H, Zheng J, Hu Z. Carcinogenesis. 2014 Aug;35(8):1901-10. Doi: 10.1093/Carcin/Bgu123. Epub 2014 May 29. Protein Tyrosine Phosphatase Receptor U (Ptpru) Is Required For Glioma Growth And Moti...
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