|Validated Species:||Chicken, Human, Mouse, Rat|
Data & Images
|Product Name||Anti-Paxillin Antibody (Monoclonal, PXC-10)|
|Description||Mouse IgG monoclonal antibody for Paxillin, paxillin (PXN) detection. Tested with WB, ICC in Human;mouse;rat;chicken. No cross reactivity with other proteins.|
|Cite This Product||Anti-Paxillin Antibody (Monoclonal, PXC-10) PXC-10 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1080)|
|Replacement Item||This antibody may replace the following items: sc-135171|sc-135170|sc-47366|sc-47364|sc-56228|sc-47368|sc-374639 from Santa Cruz Biotechnology.|
|Validated Species||Chicken, Human, Mouse, Rat|
|Predicted Species||Canine, Horse, Rabbit|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||C-terminal part of recombinant chicken paxillin(amino acids 305-559).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||64019 MW|
|Protein Function||Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). .|
|Sequence Similarities||Belongs to the paxillin family.|
|Subcellular Localization||Cytoplasm, cytoskeleton . Cell junction, focal adhesion . Cytoplasm, cell cortex . Colocalizes with integrins at the cell periphery. Colocalizes with PXN to membrane ruffles and the leading edge of migrating cells (By similarity). .|
|Research Areas|||signal transduction|cytoskeleton / ecm|extracellular matrix|structures|focal adhesions| signal transduction|adapters|cytoplasmic| cancer||
Background for Paxillin
Anti-Paxillin Antibody (Monoclonal, PXC-10) Images
Click the images to enlarge.
Lane 1: 293T Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: MM231 Cell Lysate
Lane 5: JURKAT Cell Lysate