Anti-Paxillin Antibody (Monoclonal, PXC-10)
|Reactivity||Chicken, Human, Mouse, Rat|
|Ig Isotype||Mouse IgG1|
|Product Name||Anti-Paxillin Antibody (Monoclonal, PXC-10)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for Paxillin, paxillin (PXN) detection. Tested with WB, ICC in Human;mouse;rat;chicken. No cross reactivity with other proteins.|
|Cite This Product||Anti-Paxillin Antibody (Monoclonal, PXC-10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1080)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||C-terminal part of recombinant chicken paxillin(amino acids 305-559).|
|Reactivity||Chicken, Human, Mouse, Rat|
Assay Dilutions Overview
Immunocytochemistry , 1μg/ml, Human, mouse, rat, chicken, -
Western blot, 1-2μg/ml, Human, mouse, rat, chicken
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.
Images And Assay Conditions
Anti-Paxillin antibody (monoclonal), MA1080, Western blotting
Lane 1: 293T Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: MM231 Cell Lysate
Lane 5: JURKAT Cell Lysate
Protein Target Info (Source: Uniprot.org)
|Subcellular Localization||Cytoplasm, cytoskeleton . Cell junction, focal adhesion . Cytoplasm, cell cortex . Colocalizes with integrins at the cell periphery. Colocalizes with PXN to membrane ruffles and the leading edge of migrating cells (By similarity). .|
|Molecular Weight||64019 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). .|
|Research Areas||Chicken, Human, Mouse, Rat
*You can search these to find other products in these research areas.
|Background||The paxillin gene can be alternatively spliced to include 1 of 2 alternative exons, generating beta and gamma isoforms. Paxillin is a 68-kDa focal adhesion protein that is phosphorylated on tyrosine residues in fibroblasts in response to transformation by v-src, treatment with platelet-derived growth factor, or cross-linking of integrins. The 68-kD protein(paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney(MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells.|
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Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact email@example.com
Q: Can you provide monoclonal antibodies carrier free?A: For most of our monoclonal antibodies, we are unable to provide them carrier free. If you would like to check if a specific antibody can be provided carrier free, please send an email to firstname.lastname@example.org.
Q: What are some alternative names that could be used to describe this product?A: Some common names include but are not limited to paxillin antibody, pax1 antibody