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Product Info Summary
| SKU: | M01561 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Integrin alpha V ITGAV Rabbit Monoclonal Antibody
SKU/Catalog Number
M01561
BM5155 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Integrin alpha V ITGAV Rabbit Monoclonal Antibody catalog # M01561. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Integrin alpha V ITGAV Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01561)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABGC-9
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Integrin alpha V
Reactive Species
M01561 is reactive to ITGAV in Human, Mouse, Rat
Observed Molecular Weight
69 kDa
Calculated molecular weight
116.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01561 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: human A549 whole cell, human A431 whole cell, human 293T whole cell, human MCF-7 whole cell, rat spleen tissue, rat brain tissue, mouse spleen tissue, mouse brain tissue
Validation Images & Assay Conditions
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Western blot analysis of ITGAV using anti-ITGAV antibody (M01561).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat spleen tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse spleen tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGAV antigen affinity purified monoclonal antibody (Catalog # M01561) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITGAV at approximately 130, 140, 150 kDa. The expected band size for ITGAV is at 116 kDa.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis using the Antibody at 1:500 dilution.
Specific Publications For Anti-Integrin alpha V ITGAV Rabbit Monoclonal Antibody (M01561)
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Customer Q&As
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4 Customer Q&As for Anti-Integrin alpha V ITGAV Rabbit Monoclonal Antibody
Question
My question regards to test anti-Integrin alpha V Rabbit Monoclonal antibody M01561 on human testis for research purposes, then I may be interested in using anti-Integrin alpha V Rabbit Monoclonal antibody M01561 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-07
Answer
The products we sell, including anti-Integrin alpha V Rabbit Monoclonal antibody M01561, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-07
Question
Would anti-Integrin alpha V Rabbit Monoclonal antibody M01561 work on primate WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
Our lab technicians have not tested anti-Integrin alpha V Rabbit Monoclonal antibody M01561 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Integrin alpha V Rabbit Monoclonal antibody M01561 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-25
Question
I see that the anti-Integrin alpha V Rabbit Monoclonal antibody M01561 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-06-12
Question
Is this M01561 anti-Integrin alpha V Rabbit Monoclonal antibody reactive to the isotypes of ITGAV?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
The immunogen of M01561 anti-Integrin alpha V Rabbit Monoclonal antibody is A synthesized peptide derived from human Integrin alpha V. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-05
