Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2)

SKU MA1081
Size 100μg/vial
Reactivity Human, Rat
Clonality Monoclonal
Clone Number C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2
Host Mouse
Ig Isotype Mouse IgG2a/ IgG1
Applications IHC, WB

Overview

Product Name Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2)
SKU/Catalog Number MA1081
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for PCK detection. Tested with WB, IHC-P, IHC-F in Human;rat. No cross reactivity with other proteins.
Cite This Product Anti-PCK Antibody (Monoclonal, C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1081)
Host Mouse
Immunogen mixture of several monoclonal cytokeratin clones.
Reactivity Human, Rat

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Paraffin-embedded Section), 0.1-0.5μg/ml, Human, rat, By Heat
Immunohistochemistry(Frozen Section), 0.1-0.5μg/ml, Human, rat, -
Western blot, 1-2μg/ml, Human, rat

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).

Images And Assay Conditions

/antibody/ma1081 1 IHC anti pck antibody monoclonal c 11 pck 26 cy 90 ks 1a3 m20 a53 b a2.jpg

Anti-PCK antibody (monoclonal), MA1081, IHC(P)
IHC(P): Human Placenta Tissue

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P04264
Gene Name KRT1
Protein Name Keratin, type II cytoskeletal 1
Tissue Specificity The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis.
Alternative Names Keratin, type II cytoskeletal 1;67 kDa cytokeratin;Cytokeratin-1;CK-1;Hair alpha protein;Keratin-1;K1;Type-II keratin Kb1;KRT1;KRTA;
Subcellular Localization Cell membrane . Located on plasma membrane of neuroblastoma NMB7 cells.
Molecular Weight 66039 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK. .
Background Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin(mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues.

Order Product (MA1081)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280

USD $280

Ships in 5-7 business days.

Troubleshooting

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Publications

A metanephric adenoma of the kidney associated with polycythemia: A case report
Huang, D., Wu, J., Su, X., Yan, H., & Sun, Z. (2017). Effects of low dose of bisphenol A on the proliferation and mechanism of primary cultured prostate epithelial cells in rodents. Oncology Letters. doi: 10.3892/ol.2017.6469
Bai J, Liu H, Li D, Cui L, Wu X. Plos One. 2012;7(11):E49673. Doi: 10.1371/Journal.Pone.0049673. Epub 2012 Nov 20. Duodenum Clamping Trauma Induces Significant Postoperative Intraperitoneal Adhesions On A Rat Model.

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.