Anti-EGFR Antibody Picoband™

EGFR antibody

Boster Bio Anti-EGFR Antibody Picoband™ catalog # A00023-5. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. Cited in 2 publication(s).

Product Info Summary

SKU: A00023-5
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-EGFR Antibody Picoband™

View all EGFR Antibodies

SKU/Catalog Number

A00023-5

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-EGFR Antibody Picoband™ catalog # A00023-5. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-EGFR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00023-5)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human EGFR recombinant protein (Position: D1006-A1210).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00023-5 is reactive to EGFR in Human

Applications

A00023-5 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

180 kDa

Calculated molecular weight

134.277kDa

Background of EGFR

The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGFalpha). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For EGFR (Source: Uniprot.org, NCBI)

Gene Name

EGFR

Full Name

Epidermal growth factor receptor

Weight

134.277kDa

Superfamily

protein kinase superfamily

Alternative Names

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 EGFR ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA epidermal growth factor receptor epidermal growth factor receptor|avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor tyrosine kinase domain|erb-b2 receptor tyrosine kinase 1|proto-oncogene c-ErbB-1|receptor tyrosine-protein kinase erbB-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on EGFR, check out the EGFR Infographic

EGFR infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EGFR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00023-5 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Epidermal Growth Factor Receptor in Cultured Human Retinal Pigment Epithelial Cells

Inhibition of angiogenesis involves in anticancer activity of riccardin D, a macrocyclic bisbibenzyl, in human lung carcinoma

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Order DetailsPrice
A00023-5

100μg

$370
A00023-5-10ug

10μg sample (liquid)

$99
A00023-5-Biotin

100 μg Biotin conjugated

$570
A00023-5-Cy3

100 μg Cy3 conjugated

$570
A00023-5-Dylight488

100 μg Dylight488 conjugated

$570
A00023-5-Dylight550

100 μg Dylight550 conjugated

$570
A00023-5-Dylight594

100 μg Dylight594 conjugated

$570
A00023-5-FITC

100 μg FITC conjugated

$570
A00023-5-HRP

100 μg HRP conjugated

$570
A00023-5-APC

100 μg APC conjugated

$670
A00023-5-PE

100 μg PE conjugated

$670
A00023-5-carrier-free

Carrier Free

$370

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A00023-5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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