Anti-IL18 Antibody Picoband™

Boster Bio Anti-IL18 Antibody Picoband™ catalog # PB9006. Tested in IHC-P, ICC, WB applications. This antibody reacts with Human. Cited in 4 publication(s).

Product Info Summary

SKU: PB9006
Size: 100μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC-P, ICC, WB

Product Name

Anti-IL18 Antibody Picoband™

See all IL-18/IL-1F4 products

SKU/Catalog Number

PB9006

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IL18 Antibody Picoband™ catalog # PB9006. Tested in IHC-P, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IL18 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9006)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human IL-18 recombinant protein (Position: Y37-D193). Human IL-18 shares 66% and 64% amino acid (aa) sequences identity with mouse and rat IL-18, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9006 is reactive to IL18 in Human

Applications

PB9006 is guaranteed for IHC-P, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For IL18 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q14116

Gene ID

3606

Gene Name

IL18

Full Name

Interleukin-18

Weight

22.326kDa

Superfamily

IL-1 family

Alternative Names

Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18 IL18 IGIF, IL-18, IL-1g, IL1F4 interleukin 18 interleukin-18|IFN-gamma-inducing factor|IL-1 gamma|iboctadekin|interleukin 18 (interferon-gamma-inducing factor)|interleukin-1 gamma

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL18, check out the IL18 Infographic

IL18 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL18: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PB9006 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The effect of salusin-? on expression of pro- and anti-inflammatory cytokines in human umbilical vein endothelial cells (HUVECs)

Yang Yj, Shen Y, Chen Sh, Ge Xr. World J Gastroenterol. 2005 Aug 7;11(29):4524-9. Role Of Interleukin 18 In Acute Lung Inflammation Induced By Gut Ischemia Reperfusion.

Wang Y, Zhang X, Zhang Y, Xu H, Fang G. J Reprod Immunol. 2011 Dec;92(1-2):45-53. Doi: 10.1016/J.Jri.2011.07.002. Epub 2011 Sep 13. Expression And Localization Of Il-18 In The Hypothalamic-Pituitary-Ovarian Axis Of Non-Pregnant, Pregnant, And Abor...

Si Lf, Zhang Sy, Gao Cs, Chen Sl, Zhao J, Cheng Xc. Asian-Australas J Anim Sci. 2013 Oct;26(10):1399-405. Doi: 10.5713/Ajas.2013.13101. Effects Of Ifn-?? On Il-18 Expression In Pregnant Rats And Pregnancy Outcomes.

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19 Customer Q&As for Anti-IL18 Antibody Picoband™

Question

Since the mature form of IL-18 was used as immunogen for PB9006, have you ever done any internal testing about its reacitivity with the active form after processing by CASP?

Verified customer

Asked: 2020-11-09

Answer

For the Anti-IL18 Antibody Picoband | Bosterbio (PB9006), our lab hasn't validated reacitivity with the active form after processing by CASP.

Boster Scientific Support

Answered: 2020-11-10

Question

Is a blocking peptide available for product anti-IL18 antibody (PB9006)?

Verified Customer

Verified customer

Asked: 2020-03-03

Answer

We do provide the blocking peptide for product anti-IL18 antibody (PB9006). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-03-03

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for peripheral blood using anti-IL18 antibody PB9006. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-01-20

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-20

Question

What form of IL-18 does PB9006 detect?

Verified Customer

Verified customer

Asked: 2019-10-29

Answer

According to the molecular weight of the target band our lab technicians detected, 22kD, PB9006 detects the native pro-form of IL-18. <br> The molecular weight of mature form of IL-18 is 18KD.

Boster Scientific Support

Answered: 2019-10-29

Question

I was wanting to use your anti-IL18 antibody for ICC for human peripheral blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human peripheral blood identification?

Verified Customer

Verified customer

Asked: 2019-09-12

Answer

As indicated on the product datasheet, PB9006 anti-IL18 antibody has been tested for IHC-P, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human peripheral blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-12

Question

We have seen staining in human liver. Do you have any suggestions? Is anti-IL18 antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2019-07-23

Answer

From literature liver does express IL18. From Uniprot.org, IL18 is expressed in lower esophagus mucosa, liver, urinary bladder, peripheral blood, among other tissues. Regarding which tissues have IL18 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 8666798, 15326478
Peripheral blood, Pubmed ID: 10653850
Urinary bladder, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-07-23

Question

I am interested in to test anti-IL18 antibody PB9006 on human peripheral blood for research purposes, then I may be interested in using anti-IL18 antibody PB9006 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-07-08

Answer

The products we sell, including anti-IL18 antibody PB9006, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-07-08

Question

Is this PB9006 anti-IL18 antibody reactive to the isotypes of IL18?

Verified Customer

Verified customer

Asked: 2019-02-14

Answer

The immunogen of PB9006 anti-IL18 antibody is E.coli-derived human IL-18 recombinant protein (Position: Y37-D193). Human IL-18 shares 66% and 64% amino acid (aa) sequences identity with mouse and rat IL-18, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-02-14

Question

Will anti-IL18 antibody PB9006 work for ICC with peripheral blood?

Verified Customer

Verified customer

Asked: 2019-01-03

Answer

According to the expression profile of peripheral blood, IL18 is highly expressed in peripheral blood. So, it is likely that anti-IL18 antibody PB9006 will work for ICC with peripheral blood.

Boster Scientific Support

Answered: 2019-01-03

Question

Can you help my question with product PB9006, anti-IL18 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-10-30

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9006 anti-IL18 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-10-30

Question

Is there a BSA free version of anti-IL18 antibody PB9006 available?

Verified Customer

Verified customer

Asked: 2018-04-26

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-IL18 antibody PB9006 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-04-26

Question

We purchased anti-IL18 antibody for IHC-P on peripheral blood a few months ago. I am using human, and We intend to use the antibody for WB next. My question regards examining peripheral blood as well as lower esophagus mucosa in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2018-02-14

Answer

I looked at the website and datasheets of our anti-IL18 antibody and I see that PB9006 has been validated on human in both IHC-P and WB. Thus PB9006 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-02-14

Question

We were well pleased with the WB result of your anti-IL18 antibody. However we have been able to see positive staining in urinary bladder secreted. using this antibody. Is that expected? Could you tell me where is IL18 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2017-10-27

Answer

From what I have seen in literature, urinary bladder does express IL18. Generally IL18 expresses in secreted. Regarding which tissues have IL18 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 8666798, 15326478
Peripheral blood, Pubmed ID: 10653850
Urinary bladder, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-10-27

Question

I am Fei He as a FAS from Abcam. I am sorry to bother you. One of our customer wants to know the targets of Anti-IL-18 antibody (PB9006). Anti-IL18 antibody (ab191152) was raised against the mature human IL-18 (Y37-D193), did you test whether tested this Ab is able to detect the native pro-form of IL-18? By the way, do you have any data showing the specificity of this Ab on native mature IL-8? Thanks in advance for your help.

Verified Customer

Verified customer

Asked: 2017-10-12

Answer

According to the molecular weight of the target band our lab technicians detected, PB9006 detects the native pro-form of IL-18. https://www.bosterbio.com/anti-il-18-picoband-trade-antibody-pb9006-boster.html?options=cart The molecular weight of mature form of IL-18 is 18KD.

Boster Scientific Support

Answered: 2017-10-12

Question

I was wanting to use using your anti-IL18 antibody for positive regulation of macrophage derived foam cell differentiation studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2017-07-21

Answer

We appreciate your inquiry. This PB9006 anti-IL18 antibody is tested on hela whole cell lysate. It is guaranteed to work for IHC-P, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2017-07-21

Question

Would PB9006 anti-IL18 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-05-31

Answer

You can see on the product datasheet, PB9006 anti-IL18 antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-05-31

Question

I see that the anti-IL18 antibody PB9006 works with ICC, what is the protocol used to produce the result images on the product page?

V. Carter

Verified customer

Asked: 2015-04-15

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2015-04-15

Question

We are currently using anti-IL18 antibody PB9006 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?

J. Thomas

Verified customer

Asked: 2015-01-09

Answer

The anti-IL18 antibody (PB9006) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-01-09

Question

Here is the WB image, lot number and protocol we used for peripheral blood using anti-IL18 antibody PB9006. Please let me know if you require anything else.

B. Miller

Verified customer

Asked: 2014-10-31

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-10-31

Conjugation

how to order through PO

Size

Total: $280

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PB9006
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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