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Product Info Summary
| SKU: | PB9014 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-IL18 Antibody Picoband®
View all IL-18/IL-1F4 Antibodies
SKU/Catalog Number
PB9014
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL18 Antibody Picoband® catalog # PB9014. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL18 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9014)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.01 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat IL-18 recombinant protein (Position: H37-S194). Rat IL-18 shares 64% and 91% amino acid (aa) sequences identity with human and mouse IL-18, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9014 is reactive to Il18 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated molecular weight
22303 MW
Background of IL-18/IL-1F4
Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids. IL18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses. IL18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9014 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat, By Heat
Flow Cytometry(Fixed), 1-3μg/1x106 cells, Mouse
ELISA, 0.1-0.5μg/ml,
Positive Control
WB: mouse spleen tissue
IHC: mouse spleen tissue, rat spleen tissue
FCM: HEPA1-6 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IL18 using anti-IL18 antibody (PB9014).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL18 antigen affinity purified polyclonal antibody (Catalog # PB9014) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL18 at approximately 22 kDa. The expected band size for IL18 is at 22 kDa.
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Figure 2. IHC analysis of IL18 using anti-IL18 antibody (PB9014).
IL18 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL18 Antibody (PB9014) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of IL18 using anti-IL18 antibody (PB9014).
IL18 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-IL18 Antibody (PB9014) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 4. Flow Cytometry analysis of HEPA1-6 cells using anti-L18 antibody (PB9014).
Overlay histogram showing HEPA1-6 cells stained with PB9014 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-L18 Antibody (PB9014, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For Il18 (Source: Uniprot.org, NCBI)
Gene Name
Il18
Full Name
Interleukin-18
Weight
22303 MW
Superfamily
IL-1 family
Alternative Names
Interleukin-18;IL-18;Interferon gamma-inducing factor;IFN-gamma-inducing factor;Interleukin-1 gamma;IL-1 gamma;Il18;Igif; IL18 IGIF, IL-18, IL-1g, IL1F4 interleukin 18 interleukin-18|IFN-gamma-inducing factor|IL-1 gamma|iboctadekin|interleukin 18 (interferon-gamma-inducing factor)|interleukin-1 gamma
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Il18, check out the Il18 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Il18: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL18 Antibody Picoband® (PB9014)
Hello CJ!
PB9014 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yang Yj, Shen Y, Chen Sh, Ge Xr. World J Gastroenterol. 2005 Aug 7;11(29):4524-9. Role Of Interleukin 18 In Acute Lung Inflammation Induced By Gut Ischemia Reperfusion.
Wang Y, Zhang X, Zhang Y, Xu H, Fang G. J Reprod Immunol. 2011 Dec;92(1-2):45-53. Doi: 10.1016/J.Jri.2011.07.002. Epub 2011 Sep 13. Expression And Localization Of Il-18 In The Hypothalamic-Pituitary-Ovarian Axis Of Non-Pregnant, Pregnant, And Abor...
Si Lf, Zhang Sy, Gao Cs, Chen Sl, Zhao J, Cheng Xc. Asian-Australas J Anim Sci. 2013 Oct;26(10):1399-405. Doi: 10.5713/Ajas.2013.13101. Effects Of Ifn-?? On Il-18 Expression In Pregnant Rats And Pregnancy Outcomes.
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Customer Reviews
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Customer Q&As
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18 Customer Q&As for Anti-IL18 Antibody Picoband®
Question
Please help me checked 500-P51 and PB9014 if specifically recognizes pro-IL18 or cleaved IL18 or IL18 ?
Verified customer
Asked: 2021-12-15
Answer
The antibody recognizes IL-18 and it doesn't specifically recognizes pro-IL18 or cleaved IL18. Please see the WB result image on the product page. https://www.bosterbio.com/anti-il-18-picoband-trade-antibody-pb9014-boster.html
Boster Scientific Support
Answered: 2021-12-15
Question
Is this PB9014 anti-IL18 antibody reactive to the isotypes of IL18?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
The immunogen of PB9014 anti-IL18 antibody is E.coli-derived rat IL-18 recombinant protein (Position: H37-S194). Rat IL-18 shares 64% and 91% amino acid (aa) sequences identity with human and mouse IL-18, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-30
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lower esophagus mucosa using anti-IL18 antibody PB9014. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-03
Question
Will anti-IL18 antibody PB9014 work on zebrafish WB with liver?
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
Our lab technicians have not validated anti-IL18 antibody PB9014 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-IL18 antibody PB9014 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-02
Question
I was wanting to use your anti-IL18 antibody for WB for rat lower esophagus mucosa on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat lower esophagus mucosa identification?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
It shows on the product datasheet, PB9014 anti-IL18 antibody has been validated for WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lower esophagus mucosa in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-13
Question
What is the immunogen region for PB9014?
Verified customer
Asked: 2019-10-23
Answer
The immunogen region for the Anti-IL18 Antibody Picoband™ (PB9014) is E.coli-derived rat IL-18 recombinant protein (Position: H37-S194). Rat IL-18 shares 64% and 91% amino acid (aa) sequences identity with human and mouse IL-18, respectively.
Boster Scientific Support
Answered: 2019-10-23
Question
We have been able to see staining in rat urinary bladder. What should we do? Is anti-IL18 antibody supposed to stain urinary bladder positively?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
From literature urinary bladder does express IL18. From Uniprot.org, IL18 is expressed in lower esophagus mucosa, liver, urinary bladder, peripheral blood, among other tissues. Regarding which tissues have IL18 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 8666798, 15326478
Peripheral blood, Pubmed ID: 10653850
Urinary bladder, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-10-17
Question
Can you help my question with product PB9014, anti-IL18 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9014 anti-IL18 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-18
Question
My question regards to test anti-IL18 antibody PB9014 on rat lower esophagus mucosa for research purposes, then I may be interested in using anti-IL18 antibody PB9014 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
The products we sell, including anti-IL18 antibody PB9014, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-10
Question
Would anti-IL18 antibody PB9014 work for WB with lower esophagus mucosa?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
According to the expression profile of lower esophagus mucosa, IL18 is highly expressed in lower esophagus mucosa. So, it is likely that anti-IL18 antibody PB9014 will work for WB with lower esophagus mucosa.
Boster Scientific Support
Answered: 2019-05-17
Question
Would PB9014 anti-IL18 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
As indicated on the product datasheet, PB9014 anti-IL18 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-12-18
Question
My colleagues were content with the WB result of your anti-IL18 antibody. However we have seen positive staining in liver secreted. using this antibody. Is that expected? Could you tell me where is IL18 supposed to be expressed?
M. Kulkarni
Verified customer
Asked: 2018-11-14
Answer
Based on literature, liver does express IL18. Generally IL18 expresses in secreted. Regarding which tissues have IL18 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 8666798, 15326478
Peripheral blood, Pubmed ID: 10653850
Urinary bladder, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-11-14
Question
Is a blocking peptide available for product anti-IL18 antibody (PB9014)?
Verified Customer
Verified customer
Asked: 2018-04-20
Answer
We do provide the blocking peptide for product anti-IL18 antibody (PB9014). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-04-20
Question
We are currently using anti-IL18 antibody PB9014 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-06
Answer
The anti-IL18 antibody (PB9014) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-06
Question
I have attached the WB image, lot number and protocol we used for lower esophagus mucosa using anti-IL18 antibody PB9014. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-08-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-02
Question
Is there a BSA free version of anti-IL18 antibody PB9014 available?
P. Collins
Verified customer
Asked: 2016-06-09
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL18 antibody PB9014 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-06-09
Question
I see that the anti-IL18 antibody PB9014 works with WB, what is the protocol used to produce the result images on the product page?
F. Krishna
Verified customer
Asked: 2015-08-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-08-25
Question
I was wanting to use using your anti-IL18 antibody for lung development studies. Has this antibody been tested with western blotting on testis tissue? We would like to see some validation images before ordering.
T. Kulkarni
Verified customer
Asked: 2015-03-30
Answer
Thanks for your inquiry. This PB9014 anti-IL18 antibody is tested on rat lung tissue, brain tissue, spleen tissue, liver tissue, testis tissue. It is guaranteed to work for WB in rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-03-30
