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- Table of Contents
Perforin-1 Antibodies
AND PRF1 ELISA kits, Perforin-1 Proteins
Many biological assays use antibodies to detect Perforin-1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Perforin-1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Perforin-1 antibodies...
Designing a Western blot for Perforin-1? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the PRF1 Western Blot GuidePRF1 Infographic by Boster Bio
All PRF1 Products
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Specificity
Applications
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PRF1 Overview
Facts about Perforin-1.
Perforin-1 (PRF1)
Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells which are recognized as non-self by the immune system, e.
g. in transplant rejection or any forms of autoimmune disease.
Gene Information
| Human | |
|---|---|
| Gene Name: | PRF1 |
| Uniprot: | P14222 |
| Entrez: | 5551 |
Family
| Belongs to: |
|---|
| complement C6/C7/C8/C9 family |
Alternative Names
Cytolysin; FLH2; HPLH2; HPLH2lymphocyte pore forming protein; Lymphocyte pore-forming protein; MGC65093; P1; P1PFN1; perforin 1 (pore forming protein); Perforin; perforin-1; PFP; PFPcytolysin; PRF1
Molecular Weight
Mass (kDA):
61.377 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 10q22.1 |
| Sequence: | 10; NC_000010.11 (70597348..70602788, complement) |
Subcellular Localizations
Cytoplasmic granule lumen. Secreted. Cell membrane; Multi-pass membrane protein. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.
Diseases
- Lymphohistiocytosis, Hemophagocytic
- Familial Hemophagocytic Lymphocytosis
- Neoplasms
- Malignant Neoplasms
- Histiocytosis, Non-langerhans-cell
- Epstein-barr Virus Infections
- Virus Diseases
- Lymphoma
- Infective Disorder
- Immunologic Deficiency Syndromes
- Inflammation
Interacting Proteins
- GZMB
- KRT31
Pathways
- Pathogenesis
- Mating
- Methylation
- Dna Methylation
- Immune Response
- Exocytosis
- Virulence
- Cell Proliferation
- Macrophage Activation
- Conjugation
- Demethylation
- Cytokine Production
- Cell Activation
PTMs
- Methylation
- Phosphorylation
- Demethylation
- Reduction
Research Areas
- Innate Immunity
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/PRF1
bosterbio.com/bosterbio-gene-info-cards/PRF1
References
PMID: 3419519 by Lichtenheld M.G., et al. Structure and function of human perforin.
PMID: 2592021 by Shinkai Y., et al. Molecular cloning and chromosomal assignment of a human perforin (PFP) gene.