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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human IL-18 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0864)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: Y33-D197
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0864 is reactive to IL18 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0864 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0864-CAP||Anti-Human IL18 Pre-coated 96-well strip microplate||1|
|EK0864-ST||Human IL18 Standard||2 vials, 10 ng/tube|
|EK0864-DA||Human IL18 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
Click image to see more details
Human IL-18 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of IL-18/IL-1F4 in ELISA kits from four different production batches/lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For IL18 (Source: Uniprot.Org, NCBI)
Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18 IL18 IGIF, IL-18, IL-1g, IL1F4 interleukin 18 interleukin-18|IFN-gamma-inducing factor|IL-1 gamma|iboctadekin|interleukin 18 (interferon-gamma-inducing factor)|interleukin-1 gamma*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on IL18, check out the IL18 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IL18: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0864 has been cited in 17 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Shen R, Yin P, Yao H, Chen L, Chang X, Li H, Hou X. Punicalin Ameliorates Cell Pyroptosis Induced by LPS/ATP Through Suppression of ROS/NLRP3 Pathway. J Inflamm Res. 2021 Mar 5;14:711-718. doi: 10.2147/JIR.S299163. PMID: 33707964; PMCID: PMC7943540.
EK0864 usage in article: APP:ELISA, SAMPLE: J774A.1 CELL, DILUTION:NA
Jia M,Lv Y,Xu Y,Gong Z.A comparative analysis of NLRP3-related inflammatory mediators in synovial fluid in temporomandibular joint osteoarthritis and internal derangement.BMC Musculoskelet Disord.2021 Feb 26;22(1):229.doi:10.1186/s12891-021-04092-0.PMID:3
EK0864 usage in article: APP:ELISA, SAMPLE:SYNOVIAL FLUID, DILUTION:NA
The effectiveness of olmesartan on inflammation at cardiopulmonary bypass. Ibrahim Murat Ozguler,Latif Ustunel
EK0864 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Mengying Jia,Yaoguang Lv,Yingjie Xu et al.Pyroptosis Related Molecules are Significant for Cartilage Degeneration, 23 November 2020, PREPRINT (Version 1) available at Research Square [+https://doi.org/10.21203/rs.3.rs-104795/v1+]
Yang, Q., Liu, Q., Lv, H., Wang, F., Liu, R., & Zeng, N. (2020). Effect of pulegone on the NLPR3 inflammasome during inflammatory activation of THP‑1 cells. Experimental and Therapeutic Medicine, 19(2), 1304-1312.
Interleukin 18 Polymorphisms and its serum level in Patients with Multiple Sclerosis.
Do neutrophil gelatinase-associated lipocalin and interleukin-18 predict renal dysfunction in patients with familial Mediterranean fever and amyloidosis?
Accuracy of plasma interleukin-18 and adiponectin concentrations in predicting metabolic syndrome and cardiometabolic disease risk in middle-age Brazilian men
Antitumor Effects of Berberine on Gliomas via Inactivation of Caspase-1-Mediated IL-1β and IL-18 Release
Altered expression of interleukin-18 in the ectopic and eutopic endometrium of women with endometriosis
The effect of salusin-? on expression of pro- and anti-inflammatory cytokines in human umbilical vein endothelial cells (HUVECs)
Interplay between Matrix Metalloproteinase-9, Matrix Metalloproteinase-2, and Interleukins in Multiple Sclerosis Patients
Li J, Hu SB, Wang LY, Zhang X, Zhou X, Yang B, Li JH, Xiong J, Liu N, Li Y, Wu YZ, Zheng QC. Oncogene. 2017 Nov 30;36(48):6725-6737. doi: 10.1038/onc.2017.272. Epub 2017 Aug 7. Autophagy-dependent generation of Axin2+ cancer stem-like cells promot...
Zhao Y, Shen M, Feng Y, He R, Xu X, Xie Y, Shi X, Zhou M, Pan S, Wang M, Guo X, Qin R. Oncotarget. 2017 Dec 7;9(19):14803-14814. doi: 10.18632/oncotarget.22976. eCollection 2018 Mar 13. Regulatory B cells induced by pancreatic cancer cell-derived ...
Wan L, Yuan X, Liu M, Xue B. Exp Ther Med. 2018 Mar;15(3):2429-2435. doi: 10.3892/etm.2017.5667. Epub 2017 Dec 21. miRNA-223-3p regulates NLRP3 to promote apoptosis and inhibit proliferation of hep3B cells
Wang Y, Qin Y, Wang T, Chen Y, Lang X, Zheng J, Gao S, Chen S, Zhong X, Mu Y, Wu X, Zhang F, Zhao W, Zhong Z. Sci Rep. 2018 Feb 13;8(1):2887. doi: 10.1038/s41598-018-20958-1. Pyroptosis induced by enterovirus 71 and coxsackievirus B3 infection af...
Esfahani, M., Saidijam, M., Goodarzi, M.T., Movahedian, A., & Najafi, R. (2017). Salusin-α Attenuates Inflammatory Responses in Vascular Endothelial Cells. Biochemistry (Moscow), 82(11), 1314–1323. doi: 10.1134/S0006297917110098
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