Human IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine™

Human IL-18 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. Cited in 19 publication(s).

Product Info Summary

SKU: EK0864
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine™

See all IL-18/IL-1F4 products

SKU/Catalog Number

EK0864

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL-18 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0864)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Y33-D197

Sensitivity

<1 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0864 is reactive to IL18 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0864 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0864-CAP Anti-Human IL18 Pre-coated 96-well strip microplate 1
EK0864-ST Human IL18 Standard 2 vials, 10 ng/tube
EK0864-DA Human IL18 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0330.1050.1320.2160.3640.6761.2351.955

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3324752036254548
Standard deviation2.413.8321.842.6616.2531.78
CV (%)7.3%5.6%4.2%7.4%6.4%5.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-18/IL-1F4 in ELISA kits from four different production batches/lots.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 133323331320.822.5%
Sample 224721222123222813.055.7%
Sample 352053356254647831.155.4%
*number of samples for each test n=16.

Gene/Protein Information For IL18 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q14116

Gene ID

3606

Gene Name

IL18

Full Name

Interleukin-18

Weight

22.326kDa

Superfamily

IL-1 family

Alternative Names

Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18 IL18 IGIF, IL-18, IL-1g, IL1F4 interleukin 18 interleukin-18|IFN-gamma-inducing factor|IL-1 gamma|iboctadekin|interleukin 18 (interferon-gamma-inducing factor)|interleukin-1 gamma

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL18, check out the IL18 Infographic

IL18 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL18: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0864 has been cited in 19 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Beneficial Effect of Pistachio Consumption on Glucose Metabolism, Insulin Resistance, Inflammation, and Related Metabolic Risk Markers: A Randomized Clinical Trial
Species: Human

Deubiquitination of NLRP6 inflammasome by Cyld critically regulates intestinal inflammation
Species: Human

Shen R, Yin P, Yao H, Chen L, Chang X, Li H, Hou X. Punicalin Ameliorates Cell Pyroptosis Induced by LPS/ATP Through Suppression of ROS/NLRP3 Pathway. J Inflamm Res. 2021 Mar 5;14:711-718. doi: 10.2147/JIR.S299163. PMID: 33707964; PMCID: PMC7943540.
Species: Mouse
EK0864 usage in article: APP:ELISA, SAMPLE: J774A.1 CELL, DILUTION:NA

Jia M,Lv Y,Xu Y,Gong Z.A comparative analysis of NLRP3-related inflammatory mediators in synovial fluid in temporomandibular joint osteoarthritis and internal derangement.BMC Musculoskelet Disord.2021 Feb 26;22(1):229.doi:10.1186/s12891-021-04092-0.PMID:3
Species: Human
EK0864 usage in article: APP:ELISA, SAMPLE:SYNOVIAL FLUID, DILUTION:NA

The effectiveness of olmesartan on inflammation at cardiopulmonary bypass. Ibrahim Murat Ozguler,Latif Ustunel
Species: Human
EK0864 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Mengying Jia,Yaoguang Lv,Yingjie Xu et al.Pyroptosis Related Molecules are Significant for Cartilage Degeneration, 23 November 2020, PREPRINT (Version 1) available at Research Square [+https://doi.org/10.21203/rs.3.rs-104795/v1+]
Species: Human

Yang, Q., Liu, Q., Lv, H., Wang, F., Liu, R., & Zeng, N. (2020). Effect of pulegone on the NLPR3 inflammasome during inflammatory activation of THP‑1 cells. Experimental and Therapeutic Medicine, 19(2), 1304-1312.

Interleukin 18 Polymorphisms and its serum level in Patients with Multiple Sclerosis.

Do neutrophil gelatinase-associated lipocalin and interleukin-18 predict renal dysfunction in patients with familial Mediterranean fever and amyloidosis?

Accuracy of plasma interleukin-18 and adiponectin concentrations in predicting metabolic syndrome and cardiometabolic disease risk in middle-age Brazilian men

Antitumor Effects of Berberine on Gliomas via Inactivation of Caspase-1-Mediated IL-1β and IL-18 Release

Altered expression of interleukin-18 in the ectopic and eutopic endometrium of women with endometriosis

The effect of salusin-? on expression of pro- and anti-inflammatory cytokines in human umbilical vein endothelial cells (HUVECs)

Interplay between Matrix Metalloproteinase-9, Matrix Metalloproteinase-2, and Interleukins in Multiple Sclerosis Patients

Li J, Hu SB, Wang LY, Zhang X, Zhou X, Yang B, Li JH, Xiong J, Liu N, Li Y, Wu YZ, Zheng QC. Oncogene. 2017 Nov 30;36(48):6725-6737. doi: 10.1038/onc.2017.272. Epub 2017 Aug 7. Autophagy-dependent generation of Axin2+ cancer stem-like cells promot...

Zhao Y, Shen M, Feng Y, He R, Xu X, Xie Y, Shi X, Zhou M, Pan S, Wang M, Guo X, Qin R. Oncotarget. 2017 Dec 7;9(19):14803-14814. doi: 10.18632/oncotarget.22976. eCollection 2018 Mar 13. Regulatory B cells induced by pancreatic cancer cell-derived ...

Wan L, Yuan X, Liu M, Xue B. Exp Ther Med. 2018 Mar;15(3):2429-2435. doi: 10.3892/etm.2017.5667. Epub 2017 Dec 21. miRNA-223-3p regulates NLRP3 to promote apoptosis and inhibit proliferation of hep3B cells

Wang Y, Qin Y, Wang T, Chen Y, Lang X, Zheng J, Gao S, Chen S, Zhong X, Mu Y, Wu X, Zhang F, Zhao W, Zhong Z. Sci Rep. 2018 Feb 13;8(1):2887. doi: 10.1038/s41598-018-20958-1. Pyroptosis induced by enterovirus 71 and coxsackievirus B3 infection af...

Esfahani, M., Saidijam, M., Goodarzi, M.T., Movahedian, A., & Najafi, R. (2017). Salusin-α Attenuates Inflammatory Responses in Vascular Endothelial Cells. Biochemistry (Moscow), 82(11), 1314–1323. doi: 10.1134/S0006297917110098

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8 Customer Q&As for Human IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine™

Question

Q: Can IL-18 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

G. Wilson

Verified customer

Asked: 2020-04-19

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-04-19

Question

Q: if the enzyme conjugated IL-18 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-18 antigen?

J. Taylor

Verified customer

Asked: 2020-01-01

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-01-01

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-04-10

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-04-10

Question

Q: is it okay to use citrate plasma as samples in Human IL-18 Picokine® ELISA Kit (Catalog # EK0864)?

Verified Customer

Verified customer

Asked: 2018-10-31

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of IL-18 causing disruption of its protein structure. IL-18 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IL-18 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-10-31

Question

Q: how to proceed with the analysis of ELISA data? I measured IL-18 level in plasma.

Verified Customer

Verified customer

Asked: 2018-06-12

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-06-12

Question

Which isoform can be detected by this kit?

Verified Customer

Verified customer

Asked: 2018-01-26

Answer

It can detect both mature IL-18 and precursor IL-18.

Boster Scientific Support

Answered: 2018-01-26

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2016-09-28

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2016-09-28

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-09-25

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-09-25

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