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Product Info Summary
| SKU: | M03918 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-Perilipin A PLIN1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03918
BM5033 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Perilipin A PLIN1 Rabbit Monoclonal Antibody catalog # M03918. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Perilipin A PLIN1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03918)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAED-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Perilipin A
Reactive Species
M03918 is reactive to PLIN1 in Human, Mouse
Observed Molecular Weight
62 kDa
Calculated molecular weight
56.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03918 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of Perilipin A expression in human fetal liver lysate.
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Western blot analysis of PLIN1 using anti-PLIN1 antibody (M03918).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1-3: mouse Brown Adipose Tissue-f/f,
Lane 4-6: mouse Brown Adipose Tissue-mko,
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLIN1 antigen affinity purified polyclonal antibody (A04887-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Goat Anti-Rabbit IgG Antibody at a dilution of 1:5000 for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Abbkine system.The expected band size for PLIN1 is at 56 kDa.
Specific Publications For Anti-Perilipin A PLIN1 Rabbit Monoclonal Antibody (M03918)
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Customer Reviews
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1 Reviews For Anti-Perilipin A PLIN1 Rabbit Monoclonal Antibody
This antibody shows excellent consistency and reproducibility across batches, ensuring reliable and stable experimental results. Its performance remains consistent across different experiments, providing strong technical support for our research.
Excellent
| SKU | M03918 |
|---|---|
| Application | Western Blot |
| Sample | Mouse brown adipose tissue |
| Sample Processing Description | Tissue and cell proteins were extracted using RIPA buffer. After BCA quantification, 5× loading buffer was added, and the samples were boiled for 5 minutes for denaturation. |
| Primary Incubation | The membrane was incubated with the PLIN1 primary antibodies (1:1000) overnight at 4 °C. |
| Secondary Antibody | Goat Anti-Rabbit IgG Antibody (1:5000) |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Other Reagents used | Non-fat milk |
| Detection | Signal was developed using ECL substrate |
| Results Summary | This antibody shows excellent consistency and reproducibility across batches, ensuring reliable and stable experimental results. Its performance remains consistent across different experiments, providing strong technical support for our research. |
Shuai Wang, SSchool of Life Sciences, Xiamen University
Verified customer
Submitted 2025-09-25
Customer Q&As
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15 Customer Q&As for Anti-Perilipin A PLIN1 Rabbit Monoclonal Antibody
Question
Is this M03918 anti-Perilipin A Rabbit Monoclonal antibody reactive to the isotypes of PLIN1?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
The immunogen of M03918 anti-Perilipin A Rabbit Monoclonal antibody is A synthesized peptide derived from human Perilipin A. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-08
Question
My question regarding product M03918, anti-Perilipin A Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03918 anti-Perilipin A Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-19
Question
My team were satisfied with the WB result of your anti-Perilipin A Rabbit Monoclonal antibody. However we have seen positive staining in brain endoplasmic reticulum using this antibody. Is that expected? Could you tell me where is PLIN1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
Based on literature, brain does express PLIN1. Generally PLIN1 expresses in endoplasmic reticulum. Regarding which tissues have PLIN1 expression, here are a few articles citing expression in various tissues:
Adipocyte, Pubmed ID: 9521880
Brain, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-09-09
Question
I see that the anti-Perilipin A Rabbit Monoclonal antibody M03918 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-08-13
Question
Do you have a BSA free version of anti-Perilipin A Rabbit Monoclonal antibody M03918 available?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Perilipin A Rabbit Monoclonal antibody M03918 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-22
Question
Please see the WB image, lot number and protocol we used for adipocyte using anti-Perilipin A Rabbit Monoclonal antibody M03918. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-06
Question
I am looking for to test anti-Perilipin A Rabbit Monoclonal antibody M03918 on mouse adipocyte for research purposes, then I may be interested in using anti-Perilipin A Rabbit Monoclonal antibody M03918 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Miller
Verified customer
Asked: 2019-06-04
Answer
The products we sell, including anti-Perilipin A Rabbit Monoclonal antibody M03918, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-04
Question
Will anti-Perilipin A Rabbit Monoclonal antibody M03918 work for IF with adipocyte?
P. Anderson
Verified customer
Asked: 2019-03-05
Answer
According to the expression profile of adipocyte, PLIN1 is highly expressed in adipocyte. So, it is likely that anti-Perilipin A Rabbit Monoclonal antibody M03918 will work for IF with adipocyte.
Boster Scientific Support
Answered: 2019-03-05
Question
We ordered your anti-Perilipin A Rabbit Monoclonal antibody for WB on brain a few months ago. I am using mouse, and We want to use the antibody for IF next. We need examining brain as well as adipose tissue in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2018-09-18
Answer
I looked at the website and datasheets of our anti-Perilipin A Rabbit Monoclonal antibody and it seems that M03918 has been tested on mouse in both WB and IF. Thus M03918 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-09-18
Question
We are currently using anti-Perilipin A Rabbit Monoclonal antibody M03918 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-22
Answer
The anti-Perilipin A Rabbit Monoclonal antibody (M03918) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-22
Question
I was wanting to use your anti-Perilipin A Rabbit Monoclonal antibody for IF for mouse adipocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse adipocyte identification?
B. Miller
Verified customer
Asked: 2017-02-23
Answer
It shows on the product datasheet, M03918 anti-Perilipin A Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse adipocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-02-23
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adipocyte using anti-Perilipin A Rabbit Monoclonal antibody M03918. Let me know if you need anything else.
C. Johnson
Verified customer
Asked: 2016-05-05
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-05-05
Question
We have been able to see staining in mouse adipocyte. What should we do? Is anti-Perilipin A Rabbit Monoclonal antibody supposed to stain adipocyte positively?
L. Brown
Verified customer
Asked: 2016-03-17
Answer
According to literature adipocyte does express PLIN1. According to Uniprot.org, PLIN1 is expressed in adipose tissue, adipocyte, brain, liver, among other tissues. Regarding which tissues have PLIN1 expression, here are a few articles citing expression in various tissues:
Adipocyte, Pubmed ID: 9521880
Brain, Pubmed ID: 15489334
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2016-03-17
Question
Would M03918 anti-Perilipin A Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Carter
Verified customer
Asked: 2015-12-02
Answer
As indicated on the product datasheet, M03918 anti-Perilipin A Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-12-02
Question
Is a blocking peptide available for product anti-Perilipin A Rabbit Monoclonal antibody (M03918)?
K. Krishna
Verified customer
Asked: 2014-11-12
Answer
We do provide the blocking peptide for product anti-Perilipin A Rabbit Monoclonal antibody (M03918). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2014-11-12
