Anti-Peroxiredoxin 4/PRDX4 Antibody

Peroxiredoxin 4 antibody

Boster Bio Anti-Peroxiredoxin 4/PRDX4 Antibody catalog # PA1837. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1837
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-Peroxiredoxin 4/PRDX4 Antibody

View all Peroxiredoxin 4 Antibodies

SKU/Catalog Number

PA1837

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Peroxiredoxin 4/PRDX4 Antibody catalog # PA1837. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Peroxiredoxin 4/PRDX4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1837)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Peroxiredoxin 4 (253-271aa SETIIPDPAGKLKYFDKLN), identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1837 is reactive to PRDX4 in Human, Mouse, Rat

Applications

PA1837 is guaranteed for WB Boster Guarantee

Background of Peroxiredoxin 4

PRDX4 (peroxiredoxin 4) also known as AOE37-2. The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. Functional analysis showed that PRDX4 protects glutamine synthetase from inactivation. Yeast 2-hybrid, immunoprecipitation, and immunoblot analyses indicated that PRDX4 and PRDX1 are capable of homodimerization and heterodimerization with each other but not with the mitochondrial PRDX3. Gel mobility shift and immunoblot analysis found that PRDX4 depletes NFKB binding activity together with a reduction in the amounts of p50, p65, and phosphorylated IKBA, as well as a reduction in the expression of HIV-1 viral proteins. Expression of PRDX4, alone or with PRDX1, increased the resistance of yeast cells to oxidant-induced toxicity. Jin et al. (1997)suggested PRDX4 modulates IKBA phosphorylation in the cytoplasm and thus affects a peroxiredoxin-dependent redox step.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For PRDX4 (Source: Uniprot.org, NCBI)

Uniprot ID

Q13162

Gene ID

10549

Gene Name

PRDX4

Full Name

Peroxiredoxin-4

Weight

30.54kDa

Superfamily

peroxiredoxin family

Alternative Names

Antioxidant enzyme AOE372; AOE372; AOE37-2PRX-4; EC 1.11.1.15; Peroxiredoxin 4; Peroxiredoxin IV; peroxiredoxin-4; PRDX4; Prx4; prx-4; prx-IV; thioredoxin peroxidase (antioxidant enzyme); Thioredoxin peroxidase AO372; Thioredoxin-dependent peroxide reductase A0372 PRDX4 AOE37-2, AOE372, HEL-S-97n, PRX-4 peroxiredoxin 4 peroxiredoxin-4|antioxidant enzyme AOE372|epididymis secretory sperm binding protein Li 97n|peroxiredoxin IV|prx-IV|thioredoxin peroxidase (antioxidant enzyme)|thioredoxin peroxidase AO372|thioredoxin-dependent peroxide reductase A0372|thioredoxin-dependent peroxiredoxin 4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PRDX4, check out the PRDX4 Infographic

PRDX4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PRDX4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

No publications found for PA1837

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5 Customer Q&As for Anti-Peroxiredoxin 4/PRDX4 Antibody

Question

Is this PA1837 anti-Peroxiredoxin 4/PRDX4 antibody reactive to the isotypes of PRDX4?

Verified Customer

Verified customer

Asked: 2019-12-18

Answer

The immunogen of PA1837 anti-Peroxiredoxin 4/PRDX4 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Peroxiredoxin 4(253-271aa SETIIPDPAGKLKYFDKLN), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-18

Question

I have attached the WB image, lot number and protocol we used for duodenum using anti-Peroxiredoxin 4/PRDX4 antibody PA1837. Please let me know if you require anything else.

S. Edwards

Verified customer

Asked: 2018-11-07

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-11-07

Question

Would PA1837 anti-Peroxiredoxin 4/PRDX4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-10-17

Answer

You can see on the product datasheet, PA1837 anti-Peroxiredoxin 4/PRDX4 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-10-17

Question

Will anti-Peroxiredoxin 4/PRDX4 antibody PA1837 work for WB with duodenum?

K. Krishna

Verified customer

Asked: 2016-03-21

Answer

According to the expression profile of duodenum, PRDX4 is highly expressed in duodenum. So, it is likely that anti-Peroxiredoxin 4/PRDX4 antibody PA1837 will work for WB with duodenum.

Boster Scientific Support

Answered: 2016-03-21

Question

My question regards to test anti-Peroxiredoxin 4/PRDX4 antibody PA1837 on rat duodenum for research purposes, then I may be interested in using anti-Peroxiredoxin 4/PRDX4 antibody PA1837 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

V. Miller

Verified customer

Asked: 2013-12-17

Answer

The products we sell, including anti-Peroxiredoxin 4/PRDX4 antibody PA1837, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-12-17

Size

Conjugation

Total: $315

SKU:PA1837

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PA1837
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$315.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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