recombinant

AKT1 antibody (P00024-3) performs well in WB and shows reactivity with Human, Mouse, Rat.

  • Validated for inter-lot consistency, specificity, and reproducibility
  • Committed to quality since 1993
  • Over 37 Publications
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p00024 3 wbp00024 3 fig 4 1xp00024 3 fig 5 1xp00024 3 fphar 09 00674 g004p00024 3 fphar 09 00674 g003p00024 3 fphar 09 00674 g002p00024 3 fphar 10 01459 g006p00024 3 wb7p00024 3 wb8

Anti-Phospho-AKT1 (S129) Rabbit Monoclonal Antibody

AKT1 antibody

Reactive SpeciesHostApplication
Human, Mouse, RatRabbitWB

SKU: P00024-3

Size: 100 μl

Bundle to save: I do not want free secondary ab
Price:
$370/100 μl

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Quantity: 1

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Recommend Dilution

Application
Dilution
Species
WB 1:500-2000

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Product Information

Product Name
Anti-Phospho-AKT1 (S129) Rabbit Monoclonal Antibody
View all AKT1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HGD-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human AKT1
Observed Molecular Weight
60 kDa
Calculated Molecular Weight

55.7 kDa

Cite This Product
Anti-Phospho-AKT1 (S129) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00024-3)

Publications

Application:
Species:
β-Ecdysterone Attenuates Ang II–Induced Senescence in Human Aortic Smooth Muscle Cells via Autophagy Activation and ROS Suppression Through AKT/mTOR Pathway Inhibition
Published: 2026-05-09
Journal: Frontiers in Bioscience-Landmark
Application: WB
Impact Factor: 4.10
Species: Human
PMID: 42216551
YTHDF3 Mediates the Occurrence and Development of Breast Cancer by Regulating Glycolysis Through the mTOR–HIF1α–LHDA Axis
Published: 2026-04-26
Journal: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Application: WB
Impact Factor: 4.20
Species: Human
PMID: 42036761
Transcriptome analysis reveals that the injection of mesenchymal stem cells remodels extracellular matrix and complement components of the brain through PI3K/AKT/FOXO1 signaling pathway in a neuroinflammation mouse model
Published: 2025-03-21
Journal: GENOMICS
Application: WB
Impact Factor: 3.00
Species: Mouse
PMID: 40122474
Preconditioning with acteoside ameliorates myocardial ischemia‑reperfusion injury by targeting HSP90AA1 and the PI3K/Akt signaling pathway
Published: 2025-01-24
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 3.50
Species: Rat
PMID: 39886969
The Therapeutic Mechanisms of Shenyan Oral Liquid I Against Chronic Kidney Disease Based on Network Pharmacology and Experimental Validation
Published: 2024-12-01
Journal: COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING
Application: WB
Impact Factor: 1.60
Species: Rat
PMID: 37961861
Pungent agents derived from the fruits of Zanthoxylum armatum DC. are beneficial for ameliorating type 2 diabetes mellitus via regulation of AMPK/PI3K/Akt signaling
Published: 2024-04-09
Journal: Journal of Functional Foods
Application: WB
Impact Factor: 3.80
Species: Mouse
Rapamycin Regulates Lipopolysaccharide-Induced Microglial Phagocytosis In Vitro
Published: 2024-03-17
Journal: MOLECULAR BIOLOGY
Application: WB
Impact Factor: 1.50
Species: Mouse
Transcriptome, network analysis, and molecular docking approaches to elucidate the modes of action of Astragaloside IV against myocardial infarction with mitochondrial energy metabolism network-targeted regulation
Published: 2023-10-18
Journal: COMPUTERS IN BIOLOGY AND MEDICINE
Application: WB
Impact Factor: 7.70
Species: Rat
Effects and mechanisms of substance P on the proliferation and angiogenic differentiation of bone marrow mesenchymal stem cells: Bioinformatics and in vitro experiments
Published: 2023-07-07
Journal: GENOMICS
Application: WB
Impact Factor: 4.40
Species: Human
PMID: 37423397
Effects of Cooling Interventions with Different Target Temperatures on Heat Stroke Rats
Published: 2023-05-30
Journal: Journal of Inflammation Research
Application: WB
Impact Factor: 4.50
Species: Rat
PMID: 37284704
RNF113A targeted by miR-197 promotes proliferation and inhibits autophagy via CXCR4/CXCL12/AKT/ERK/Beclin1 axis in cervical cancer
Published: 2023-05-08
Journal: EXPERIMENTAL CELL RESEARCH
Application: WB
Impact Factor: 3.70
Species: Human,Mouse
PMID: 37164050
AKT/GSK-3beta/VEGF signaling is involved in P2RY2 activation-induced the proliferation and metastasis of gastric cancer
Published: 2022-12-05
Journal: CARCINOGENESIS
Application: WB
Impact Factor: 4.74
Species: Human
PMID: 36469496
Monotropein Protects against Inflammatory Bone Loss and Suppresses Osteoclast Formation and Bone Resorption by Inhibiting NFATc1 via NF-κB and Akt/GSK-3β Pathway
Published: 2022-09-24
Journal: Nutrients
Application: WB
Impact Factor: 6.71
Species: Mouse
PMID: 36235631
Integration of pharmacodynamics and metabolomics reveals the therapeutic effects of 6-acetylacteoside on ovariectomy-induced osteoporosis mice
Published: 2022-08-27
Journal: PHYTOMEDICINE
Application: WB
Impact Factor: 6.66
Species: Mouse
PMID: 36057143
Fructus Zanthoxyli extract improves glycolipid metabolism disorder of type 2 diabetes mellitus via activation of AMPK/PI3K/Akt pathway: Network pharmacology and experimental validation
Published: 2022-07-26
Journal: Journal of Integrative Medicine-JIM
Application: WB,IF
Impact Factor: 3.95
Species: Human
PMID: 35965234
Triptolide attenuates LPS-induced activation of RAW 264.7 macrophages by inducing M1-to-M2 repolarization via the mTOR/STAT3 signaling
Published: 2022-07-06
Journal: IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
Application: WB
Impact Factor: 3.71
Species: Mouse
PMID: 35793125
G protein-coupled receptor 30 mediates cell proliferation of goat mammary epithelial cells via MEK/ERK&PI3K/AKT signaling pathway
Published: 2022-06-06
Journal: CELL CYCLE
Application: WB
Impact Factor: 5.17
Species: Goat
PMID: 35659445
Duloxetine ameliorates valproic acid-induced hyperactivity, anxiety-like behavior, and social interaction deficits in zebrafish
Published: 2021-10-03
Journal: Autism Research
Application: WB
Impact Factor: 5.22
Species: Zebrafish
PMID: 34605202
Magnolol Prevents Acute Alcoholic Liver Damage by Activating PI3K/Nrf2/PPARγ and Inhibiting NLRP3 Signaling Pathway
Published: 2019-12-05
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 3.85
Species: Mouse
PMID: 31920652
Mulberry Bark Alleviates Effect of STZ Inducing Diabetic Mice through Negatively Regulating FoxO1
Published: 2019-01-21
Journal: Evidence-based Complementary and Alternative Medicine
Application: WB
Impact Factor: 1.98
Species: Mouse
PMID: 30800168
Dioscin-6′-O-acetate inhibits lung cancer cell proliferation via inducing cell cycle arrest and caspase-dependent apoptosis
Published: 2018-09-04
Journal: PHYTOMEDICINE
Application: WB
Impact Factor: 3.61
Species: Human
PMID: 30668391
Epigallocatechin-3-Gallate Promotes the Growth of Mink Hair Follicles Through Sonic Hedgehog and Protein Kinase B Signaling Pathways
Published: 2018-06-26
Journal: Frontiers in Pharmacology
Application: IF
Impact Factor: 3.83
Species: American Mink
PMID: 29997505
Essential roles of Akt/Snail pathway in microcystin-LR-induced tight junction toxicity in Sertoli cell
Published: 2018-01-04
Journal: FOOD AND CHEMICAL TOXICOLOGY
Application: WB
Impact Factor: 3.98
Species: Mouse
PMID: 29307602
Active targeting co-delivery system based on hollow mesoporous silica nanoparticles for antitumor therapy in ovarian cancer stem-like cells
Published: 2017-07-18
Journal: ONCOLOGY REPORTS
Application: WB
Impact Factor: 2.66
Species: Human
PMID: 28731164
miR-21 increases c-kit+ cardiac stem cell proliferation in vitro through PTEN/PI3K/Akt signaling
Published: 2017-01-05
Journal: PeerJ
Application: WB
Impact Factor: 2.18
Species: Rat
PMID: 28168101
miR-21 Reduces Hydrogen Peroxide-Induced Apoptosis in c-kit+ Cardiac Stem Cells In Vitro through PTEN/PI3K/Akt Signaling
Published: 2016-10-10
Journal: Oxidative Medicine and Cellular Longevity
Application: WB
Impact Factor: 4.49
Species: Rat
PMID: 27803763
CYP2J2 Overexpression Increases EETs and Protects Against HFD-Induced Atherosclerosis in ApoE−/− Mice
Published: 2016-06-01
Journal: JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
Application: WB
Impact Factor: 2.46
Species: Mouse
PMID: 26859195
Epicatechin Plus Treadmill Exercise are Neuroprotective Against Moderate-stage Amyloid Precursor Protein/Presenilin 1 Mice
Published: 2016-05-11
Journal: Pharmacognosy Magazine
Application: WB
Impact Factor: 0.83
Species: Mouse
PMID: 27279698
Afatinib inhibits proliferation and invasion and promotes apoptosis of the T24 bladder cancer cell line
Published: 2015-02-25
Journal: Experimental and Therapeutic Medicine
Application: WB
Impact Factor: 1.27
Species: Human
PMID: 26136904
Altholactone induces reactive oxygen species-mediated apoptosis in bladder cancer T24 cells through mitochondrial dysfunction, MAPK-p38 activation and Akt suppression
Published: 2014-04-03
Journal: ONCOLOGY REPORTS
Application: WB
Impact Factor: 2.19
Species: Human
PMID: 24700345
Vascular endothelial growth factor induces multidrug resistance-associated protein 1 overexpression through phosphatidylinositol-3-kinase /protein kinase B signaling pathway and transcription factor specificity protein 1 in BGC823 cell line
Published: 2013-06-13
Journal: ACTA BIOCHIMICA ET BIOPHYSICA SINICA
Application: WB
Impact Factor: 1.81
Species: Human
PMID: 23765166
Taurine attenuates oxidative stress and alleviates cardiac failure in type I diabetic rats.
Published: 2013-04-01
Journal: CROATIAN MEDICAL JOURNAL
Application: WB
Impact Factor: 1.25
Species: Rat
PMID: 23630144
A small interfering RNA targeting the KLF6 splice variant, KLF6-SV1, as gene therapy for gastric cancer
Published: 2011-05-03
Journal: Gastric Cancer
Application: WB
Impact Factor: 1.90
Species: Human
PMID: 21538018
Silencing of HIF-1α suppresses tumorigenicity of renal cell carcinoma through induction of apoptosis
Published: 2009-10-09
Journal: CANCER GENE THERAPY
Application: WB
Impact Factor: 3.01
Species: Human
PMID: 19816521
A small interfering RNA targeting osteopontin as gastric cancer therapeutics
Published: 2008-08-09
Journal: CANCER LETTERS
Application: WB
Impact Factor: 3.40
Species: Human
PMID: 18694621
Down-Regulation of Osteopontin Suppresses Growth and Metastasis of Hepatocellular Carcinoma Via Induction of Apoptosis
Published: 2008-05-07
Journal: GASTROENTEROLOGY
Application: WB
Impact Factor: 11.67
Species: Human
PMID: 18555021
Inhibition of the PI3K pathway sensitizes fludarabine-induced apoptosis in human leukemic cells through an inactivation of MAPK-dependent pathway
Published: 2005-04-05
Journal: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Application: WB
Impact Factor: 2.90
Species: Human
PMID: 15850772

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Product Promise

We promise Anti-Phospho-AKT1 (S129) Rabbit Monoclonal Antibody performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more

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P00024-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Oct 6, 2026