Anti-Phospho-ASK 1 (S966) MAP3K5 Antibody

Product Name

Anti-Phospho-ASK 1 (S966) MAP3K5 Antibody

View all ASK1 Antibodies

SKU/Catalog Number

A00929S966-2

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Phospho-ASK 1 (S966) MAP3K5 Antibody catalog # A00929S966-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-ASK 1 (S966) MAP3K5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00929S966-2)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human ASK 1 around the phosphorylation site of S966.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00929S966-2 is reactive to MAP3K5 in Human, Mouse, Rat

Applications

A00929S966-2 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

154.537kDa

Background of ASK1

Links covalently the heme group to the apoprotein of cytochrome c By similarity.

Schaefer L., Genomics 34:166-172(1996).
van den Veyver I.B., Am. J. Med. Genet. 78:179-181(1998).
The MGC Project Team; Genome Res. 14:2121-2127(2004)

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blotting validation for Anti-Phospho-ASK 1 (S966) MAP3K5 Antibody A00929S966-2

Western Blot (WB) analysis of specific cells using Phospho-ASK 1 (S966) polyclonal antibody.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For MAP3K5 (Source: Uniprot.org, NCBI)

Alternative Names

Apoptosis signal-regulating kinase 1; ASK1; ASK-1; ASK1MEKK 5; EC 2.7.11; MAP/ERK kinase kinase 5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5EC 2.7.11.25; MEK kinase 5; MEKK5; MEKK5apoptosis signal regulating kinase 1; mitogen-activated protein kinase kinase kinase 5 MAP3K5 ASK1, MAPKKK5, MEKK5 mitogen-activated protein kinase kinase kinase 5 mitogen-activated protein kinase kinase kinase 5|ASK-1|MAP/ERK kinase kinase 5|MAPK/ERK kinase kinase 5|MEK kinase 5|MEKK 5|apoptosis signal-regulating kinase 1

For more info on MAP3K5, check out the MAP3K5 Infographic

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In this infographic, you will see the following information for MAP3K5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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