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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Rat FLRT2 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat FLRT2 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Flrt2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat FLRT2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1973)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: C36-T660
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1973 is reactive to Flrt2 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK1973 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Flrt2
Fibronectin leucine-rich repeat transmembrane protein FLRT2 is a protein that in humans is encoded by the FLRT2 gene. It is mapped to 6q31. This gene encodes a member of the fibronectin leucine rich transmembrane (FLRT) family of cell adhesion molecules, which regulate early embryonic vascular and neural development. The encoded type I transmembrane protein has an extracellular region consisting of an N-terminal leucine-rich repeat domain and a type 3 fibronectin domain, followed by a transmembrane domain and a short C-terminal cytoplasmic tail domain. It functions as both a homophilic cell adhesion molecule and a heterophilic chemorepellent through its interaction with members of the uncoordinated-5 receptor family. Proteolytic removal of the extracellular region controls the migration of neurons in the developing cortex. Alternative splicing results in multiple transcript variants.
|EK1973-CAP||Anti-Rat Flrt2 Pre-coated 96-well strip microplate||1|
|EK1973-ST||Rat Flrt2 Standard||2 vials, 10 ng/tube|
|EK1973-DA||Rat Flrt2 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat FLRT2 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Flrt2, Dilution ratio of 1:1, concentration in serum is around 40pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat FLRT2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Flrt2 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Flrt2 (Source: Uniprot.Org, NCBI)
Leucine-rich repeat transmembrane protein FLRT2
Fibronectin Leucine Rich Transmembrane Protein 2; Fibronectin-Like Domain-Containing Leucine-Rich Transmembrane Protein 2; FLRT2; KIAA0405; Leucine-Rich Repeat Transmembrane Protein FLRT2 Flrt2|fibronectin leucine rich transmembrane protein 2|leucine-rich repeat transmembrane protein FLRT2|fibronectin-like domain-containing leucine-rich transmembrane protein 2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Flrt2, check out the Flrt2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Flrt2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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