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Product Info Summary
| SKU: | P00026-2 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Phospho-c-Myc (S62) Rabbit Monoclonal Antibody
SKU/Catalog Number
P00026-2
BM4376 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-c-Myc (S62) Rabbit Monoclonal Antibody catalog # P00026-2. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-c-Myc (S62) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00026-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
EAC-13
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-c-Myc (S62)
Reactive Species
P00026-2 is reactive to MYC in Human, Mouse, Rat
Observed Molecular Weight
57 kDa
Calculated molecular weight
48.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00026-2 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: human Hela whole cell, human K562 whole cell, human MCF-7 whole cell
IHC: human lung carcinoma tissue
Validation Images & Assay Conditions
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Western blot analysis of P-MYC using anti-P-MYC antibody (P00026-2).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P-MYC antigen affinity purified monoclonal antibody (P00026-2) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for P-MYC at approximately 57 kDa. The expected band size for P-MYC is at 49 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-c-Myc (S62) Antibody .
Specific Publications For Anti-Phospho-c-Myc (S62) Rabbit Monoclonal Antibody (P00026-2)
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5 Customer Q&As for Anti-Phospho-c-Myc (S62) Rabbit Monoclonal Antibody
Question
I would like using your anti-Phospho-c-Myc (S62) Rabbit Monoclonal antibody for chromosome organization studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
Thanks for your inquiry. This P00026-2 anti-Phospho-c-Myc (S62) Rabbit Monoclonal antibody is validated on hela cell lysate. It is guaranteed to work for IP, IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-14
Question
We are currently using anti-Phospho-c-Myc (S62) Rabbit Monoclonal antibody P00026-2 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
A. Collins
Verified customer
Asked: 2017-09-21
Answer
The anti-Phospho-c-Myc (S62) Rabbit Monoclonal antibody (P00026-2) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-21
Question
We ordered your anti-Phospho-c-Myc (S62) Rabbit Monoclonal antibody for IP on uterus in the past. I am using human, and We are going to use the antibody for WB next. My lab would like examining uterus as well as cervix carcinoma erythroleukemia in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2017-06-08
Answer
I looked at the website and datasheets of our anti-Phospho-c-Myc (S62) Rabbit Monoclonal antibody and I see that P00026-2 has been validated on human in both IP and WB. Thus P00026-2 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-06-08
Question
We have been able to see staining in mouse cervix carcinoma. Are there any suggestions? Is anti-Phospho-c-Myc (S62) Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
C. Miller
Verified customer
Asked: 2016-11-28
Answer
From literature cervix carcinoma does express MYC. From Uniprot.org, MYC is expressed in thoracic mammary gland, uterus, cervix, placenta testis, promyelocytic leukemia, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have MYC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Placenta, and Testis, Pubmed ID: 15489334
Promyelocytic leukemia, Pubmed ID: 3540591
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2016-11-28
Question
My boss were content with the WB result of your anti-Phospho-c-Myc (S62) Rabbit Monoclonal antibody. However we have seen positive staining in cervix nucleus using this antibody. Is that expected? Could you tell me where is MYC supposed to be expressed?
A. Baker
Verified customer
Asked: 2013-03-05
Answer
From literature, cervix does express MYC. Generally MYC expresses in nucleus, nucleoplasm. Regarding which tissues have MYC expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Cervix, Placenta, and Testis, Pubmed ID: 15489334
Promyelocytic leukemia, Pubmed ID: 3540591
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2013-03-05
