Anti-Phospho-Eps15 (Y849) Antibody

Product Name

Anti-Phospho-Eps15 (Y849) Antibody

View all EPS15 Antibodies

SKU/Catalog Number

P01681

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Phospho-Eps15 (Y849) Antibody catalog # P01681. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Phospho-Eps15 (Y849) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P01681)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

608

Isotype

IgG

Immunogen

Synthesized peptide derived from human Eps15 around the phosphorylation site of Y849.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P01681 is reactive to EPS15 in Human, Mouse, Rat

Applications

P01681 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

98.656kDa

Background of EPS15

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser-19 which lies within the regulatory domain of TPH2 (McKinney et al., 2005).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

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Western Blot (WB) analysis of JK cells using Phospho-Eps15 (Y849) Polyclonal antibody.

Gene/Protein Information For EPS15 (Source: Uniprot.org, NCBI)

Alternative Names

AF1P; AF-1P; ALL1 fused gene from chromosome 1; epidermal growth factor receptor pathway substrate 15; epidermal growth factor receptor substrate 15; Eps15; MLLT5; Protein AF-1p; Protein Eps15 EPS15 AF-1P, AF1P, MLLT5 epidermal growth factor receptor pathway substrate 15 epidermal growth factor receptor substrate 15|ALL1 fused gene from chromosome 1|protein AF-1p

For more info on EPS15, check out the EPS15 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for EPS15: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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